Apparent Initial Enzyme Concentration in Presence of Noncompetitive Inhibitor Calculator

✖The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.ⓘ Initial Enzyme Concentration [[E₀]]			+10% -10%
✖The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.ⓘ Inhibitor Concentration [I]			+10% -10%
✖The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.ⓘ Enzyme Inhibitor Dissociation Constant [K_i]			+10% -10%

✖The Apparent Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction in the presence of a non-competitive inhibitor.ⓘ Apparent Initial Enzyme Concentration in Presence of Noncompetitive Inhibitor [E0^app]

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Formula

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Apparent Initial Enzyme Concentration in Presence of Noncompetitive Inhibitor

Formula

`"E0"^{"app"} = (("[E"_{"0"}"]")/(1+("I"/"K"_{"i"})))`

Example

`"67.85714mol/L"=("100mol/L"/(1+("9mol/L"/"19mol/L")))`

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Apparent Initial Enzyme Concentration in Presence of Noncompetitive Inhibitor Solution

STEP 0: Pre-Calculation Summary

Formula Used

Apparent Initial Enzyme Concentration = (Initial Enzyme Concentration/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))
E0^app = ([E₀]/(1+(I/K_i)))
This formula uses 4 Variables

Variables Used

Apparent Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Apparent Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction in the presence of a non-competitive inhibitor.
Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Enzyme Inhibitor Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.

STEP 1: Convert Input(s) to Base Unit

Initial Enzyme Concentration: 100 Mole per Liter --> 100000 Mole per Cubic Meter (Check conversion here)
Inhibitor Concentration: 9 Mole per Liter --> 9000 Mole per Cubic Meter (Check conversion here)
Enzyme Inhibitor Dissociation Constant: 19 Mole per Liter --> 19000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

E0^app = ([E₀]/(1+(I/K_i))) --> (100000/(1+(9000/19000)))

Evaluating ... ...

E0^app = 67857.1428571429

STEP 3: Convert Result to Output's Unit

67857.1428571429 Mole per Cubic Meter -->67.8571428571429 Mole per Liter (Check conversion here)

FINAL ANSWER

67.8571428571429 ≈ 67.85714 Mole per Liter <-- Apparent Initial Enzyme Concentration

(Calculation completed in 00.004 seconds)

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< 9 Noncompetitive Inhibitor Calculators

Dissociation Constant given Enzyme Substrate Complex Concentration

Go Enzyme Inhibitor Dissociation Constant = Inhibitor Concentration/(((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1)

Apparent Initial Enzyme Concentration in Presence of Noncompetitive Inhibitor

Go Apparent Initial Enzyme Concentration = (Initial Enzyme Concentration/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))

Initial Enzyme Concentration in presence of Noncompetitive Inhibitor

Go Initial Enzyme Concentration = (Apparent Initial Enzyme Concentration*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))

Dissociation Constant given Apparent Initial Enzyme Concentration

Go Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1))

Apparent Michaelis Menten constant given Inhibitor's Dissociation Constant

Go Apparent Michaelis Constant = Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))

Apparent Maximum Rate in presence of Noncompetitive Inhibitor

Go Apparent Maximum Rate = (Maximum Rate/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))

Dissociation Constant in presence of Noncompetitive Inhibitor

Go Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Maximum Rate/Apparent Maximum Rate)-1))

Maximum Rate in presence of Noncompetitive Inhibitor

Go Maximum Rate = (Apparent Maximum Rate*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))

Inhibitor Concentration in presence of Noncompetitive Inhibitor

Go Inhibitor Concentration = ((Maximum Rate/Apparent Maximum Rate)-1)*Enzyme Inhibitor Dissociation Constant

Apparent Initial Enzyme Concentration in Presence of Noncompetitive Inhibitor Formula

Apparent Initial Enzyme Concentration = (Initial Enzyme Concentration/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))
E0^app = ([E₀]/(1+(I/K_i)))

What is Non-competitive inhibition?

Non-competitive inhibition is a type of enzyme inhibition where the inhibitor reduces the activity of the enzyme and binds equally well to the enzyme whether or not it has already bound the substrate. In the presence of a non-competitive inhibitor, the apparent enzyme affinity is equivalent to the actual affinity because the inhibitor binds to both the enzyme and the enzyme-substrate complex equally so that the equilibrium is maintained. However, since some enzyme is always inhibited from converting the substrate to product, the effective enzyme concentration is lowered.

How to Calculate Apparent Initial Enzyme Concentration in Presence of Noncompetitive Inhibitor?

Apparent Initial Enzyme Concentration in Presence of Noncompetitive Inhibitor calculator uses Apparent Initial Enzyme Concentration = (Initial Enzyme Concentration/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))) to calculate the Apparent Initial Enzyme Concentration, The Apparent initial enzyme concentration in presence of noncompetitive inhibitor formula is defined as relation to the initial enzyme concentration, inhibitor concentration, and dissociation constant. Apparent Initial Enzyme Concentration is denoted by E0^app symbol.

How to calculate Apparent Initial Enzyme Concentration in Presence of Noncompetitive Inhibitor using this online calculator? To use this online calculator for Apparent Initial Enzyme Concentration in Presence of Noncompetitive Inhibitor, enter Initial Enzyme Concentration ([E₀]), Inhibitor Concentration (I) & Enzyme Inhibitor Dissociation Constant (K_i) and hit the calculate button. Here is how the Apparent Initial Enzyme Concentration in Presence of Noncompetitive Inhibitor calculation can be explained with given input values -> 0.067857 = (100000/(1+(9000/19000))).

FAQ

What is Apparent Initial Enzyme Concentration in Presence of Noncompetitive Inhibitor?

The Apparent initial enzyme concentration in presence of noncompetitive inhibitor formula is defined as relation to the initial enzyme concentration, inhibitor concentration, and dissociation constant and is represented as E0^app = ([E₀]/(1+(I/K_i))) or Apparent Initial Enzyme Concentration = (Initial Enzyme Concentration/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))). The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction, The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system & The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.

How to calculate Apparent Initial Enzyme Concentration in Presence of Noncompetitive Inhibitor?

The Apparent initial enzyme concentration in presence of noncompetitive inhibitor formula is defined as relation to the initial enzyme concentration, inhibitor concentration, and dissociation constant is calculated using Apparent Initial Enzyme Concentration = (Initial Enzyme Concentration/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))). To calculate Apparent Initial Enzyme Concentration in Presence of Noncompetitive Inhibitor, you need Initial Enzyme Concentration ([E₀]), Inhibitor Concentration (I) & Enzyme Inhibitor Dissociation Constant (K_i). With our tool, you need to enter the respective value for Initial Enzyme Concentration, Inhibitor Concentration & Enzyme Inhibitor Dissociation Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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