Dissociation Constant given Apparent Initial Enzyme Concentration Calculator

✖The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.ⓘ Inhibitor Concentration [I]			+10% -10%
✖The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.ⓘ Initial Enzyme Concentration [[E₀]]			+10% -10%
✖The Apparent Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction in the presence of a non-competitive inhibitor.ⓘ Apparent Initial Enzyme Concentration [E0^app]			+10% -10%

✖The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.ⓘ Dissociation Constant given Apparent Initial Enzyme Concentration [K_i]

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Formula

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Dissociation Constant given Apparent Initial Enzyme Concentration

Formula

`"K"_{"i"} = ("I"/((("[E"_{"0"}"]")/"E0"^{"app"})-1))`

Example

`"0.005403mol/L"=("9mol/L"/(("100mol/L"/"0.06mol/L")-1))`

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Dissociation Constant given Apparent Initial Enzyme Concentration Solution

STEP 0: Pre-Calculation Summary

Formula Used

Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1))
K_i = (I/(([E₀]/E0^app)-1))
This formula uses 4 Variables

Variables Used

Enzyme Inhibitor Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.
Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
Apparent Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Apparent Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction in the presence of a non-competitive inhibitor.

STEP 1: Convert Input(s) to Base Unit

Inhibitor Concentration: 9 Mole per Liter --> 9000 Mole per Cubic Meter (Check conversion here)
Initial Enzyme Concentration: 100 Mole per Liter --> 100000 Mole per Cubic Meter (Check conversion here)
Apparent Initial Enzyme Concentration: 0.06 Mole per Liter --> 60 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

K_i = (I/(([E₀]/E0^app)-1)) --> (9000/((100000/60)-1))

Evaluating ... ...

K_i = 5.4032419451671

STEP 3: Convert Result to Output's Unit

5.4032419451671 Mole per Cubic Meter -->0.0054032419451671 Mole per Liter (Check conversion here)

FINAL ANSWER

0.0054032419451671 ≈ 0.005403 Mole per Liter <-- Enzyme Inhibitor Dissociation Constant

(Calculation completed in 00.004 seconds)

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< 9 Noncompetitive Inhibitor Calculators

Dissociation Constant given Enzyme Substrate Complex Concentration

Go Enzyme Inhibitor Dissociation Constant = Inhibitor Concentration/(((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1)

Apparent Initial Enzyme Concentration in Presence of Noncompetitive Inhibitor

Go Apparent Initial Enzyme Concentration = (Initial Enzyme Concentration/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))

Initial Enzyme Concentration in presence of Noncompetitive Inhibitor

Go Initial Enzyme Concentration = (Apparent Initial Enzyme Concentration*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))

Dissociation Constant given Apparent Initial Enzyme Concentration

Go Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1))

Apparent Michaelis Menten constant given Inhibitor's Dissociation Constant

Go Apparent Michaelis Constant = Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))

Apparent Maximum Rate in presence of Noncompetitive Inhibitor

Go Apparent Maximum Rate = (Maximum Rate/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))

Dissociation Constant in presence of Noncompetitive Inhibitor

Go Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Maximum Rate/Apparent Maximum Rate)-1))

Maximum Rate in presence of Noncompetitive Inhibitor

Go Maximum Rate = (Apparent Maximum Rate*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))

Inhibitor Concentration in presence of Noncompetitive Inhibitor

Go Inhibitor Concentration = ((Maximum Rate/Apparent Maximum Rate)-1)*Enzyme Inhibitor Dissociation Constant

Dissociation Constant given Apparent Initial Enzyme Concentration Formula

Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1))
K_i = (I/(([E₀]/E0^app)-1))

What is Non-competitive inhibition?

Non-competitive inhibition is a type of enzyme inhibition where the inhibitor reduces the activity of the enzyme and binds equally well to the enzyme whether or not it has already bound the substrate. In the presence of a non-competitive inhibitor, the apparent enzyme affinity is equivalent to the actual affinity because the inhibitor binds to both the enzyme and the enzyme-substrate complex equally so that the equilibrium is maintained. However, since some enzyme is always inhibited from converting the substrate to product, the effective enzyme concentration is lowered.

How to Calculate Dissociation Constant given Apparent Initial Enzyme Concentration?

Dissociation Constant given Apparent Initial Enzyme Concentration calculator uses Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1)) to calculate the Enzyme Inhibitor Dissociation Constant, The Dissociation constant given apparent initial enzyme concentration formula is defined as relation with the initial enzyme concentration, inhibitor concentration and with apparent initial enzyme concentration. Enzyme Inhibitor Dissociation Constant is denoted by K_i symbol.

How to calculate Dissociation Constant given Apparent Initial Enzyme Concentration using this online calculator? To use this online calculator for Dissociation Constant given Apparent Initial Enzyme Concentration, enter Inhibitor Concentration (I), Initial Enzyme Concentration ([E₀]) & Apparent Initial Enzyme Concentration (E0^app) and hit the calculate button. Here is how the Dissociation Constant given Apparent Initial Enzyme Concentration calculation can be explained with given input values -> 5.4E-6 = (9000/((100000/60)-1)).

FAQ

What is Dissociation Constant given Apparent Initial Enzyme Concentration?

The Dissociation constant given apparent initial enzyme concentration formula is defined as relation with the initial enzyme concentration, inhibitor concentration and with apparent initial enzyme concentration and is represented as K_i = (I/(([E₀]/E0^app)-1)) or Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1)). The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system, The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction & The Apparent Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction in the presence of a non-competitive inhibitor.

How to calculate Dissociation Constant given Apparent Initial Enzyme Concentration?

The Dissociation constant given apparent initial enzyme concentration formula is defined as relation with the initial enzyme concentration, inhibitor concentration and with apparent initial enzyme concentration is calculated using Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1)). To calculate Dissociation Constant given Apparent Initial Enzyme Concentration, you need Inhibitor Concentration (I), Initial Enzyme Concentration ([E₀]) & Apparent Initial Enzyme Concentration (E0^app). With our tool, you need to enter the respective value for Inhibitor Concentration, Initial Enzyme Concentration & Apparent Initial Enzyme Concentration and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Enzyme Inhibitor Dissociation Constant?

In this formula, Enzyme Inhibitor Dissociation Constant uses Inhibitor Concentration, Initial Enzyme Concentration & Apparent Initial Enzyme Concentration. We can use 2 other way(s) to calculate the same, which is/are as follows -

Enzyme Inhibitor Dissociation Constant = Inhibitor Concentration/(((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1)
Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Maximum Rate/Apparent Maximum Rate)-1))

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