Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant Calculator

✖The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.ⓘ Forward Rate Constant [k_f]			+10% -10%
✖The Catalyst Concentration is the number of moles of catalyst present in the per liter of solution.ⓘ Catalyst Concentration [E]			+10% -10%
✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration [S]			+10% -10%
✖The Reverse Rate Constant is defined as the rate constant for the backward reaction.ⓘ Reverse Rate Constant [k_r]			+10% -10%
✖The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.ⓘ Catalytic Rate Constant [k_cat]			+10% -10%

✖The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.ⓘ Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant [ES]

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Formula

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Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant

Formula

`"ES" = ("k"_{"f"}*"E"*"S")/("k"_{"r"}+"k"_{"cat"})`

Example

`"12.93708mol/L"=("6.9s⁻¹"*"25mol/L"*"1.5mol/L")/("20mol/L*s"+"0.65s⁻¹")`

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Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant Solution

STEP 0: Pre-Calculation Summary

Formula Used

Enzyme Substrate Complex Concentration = (Forward Rate Constant*Catalyst Concentration*Substrate Concentration)/(Reverse Rate Constant+Catalytic Rate Constant)
ES = (k_f*E*S)/(k_r+k_cat)
This formula uses 6 Variables

Variables Used

Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Forward Rate Constant - (Measured in 1 Per Second) - The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.
Catalyst Concentration - (Measured in Mole per Cubic Meter) - The Catalyst Concentration is the number of moles of catalyst present in the per liter of solution.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Reverse Rate Constant - (Measured in Mole per Cubic Meter Second) - The Reverse Rate Constant is defined as the rate constant for the backward reaction.
Catalytic Rate Constant - (Measured in 1 Per Second) - The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.

STEP 1: Convert Input(s) to Base Unit

Forward Rate Constant: 6.9 1 Per Second --> 6.9 1 Per Second No Conversion Required
Catalyst Concentration: 25 Mole per Liter --> 25000 Mole per Cubic Meter (Check conversion here)
Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion here)
Reverse Rate Constant: 20 Mole per Liter Second --> 20000 Mole per Cubic Meter Second (Check conversion here)
Catalytic Rate Constant: 0.65 1 Per Second --> 0.65 1 Per Second No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

ES = (k_f*E*S)/(k_r+k_cat) --> (6.9*25000*1500)/(20000+0.65)

Evaluating ... ...

ES = 12937.0795449148

STEP 3: Convert Result to Output's Unit

12937.0795449148 Mole per Cubic Meter -->12.9370795449148 Mole per Liter (Check conversion here)

FINAL ANSWER

12.9370795449148 ≈ 12.93708 Mole per Liter <-- Enzyme Substrate Complex Concentration

(Calculation completed in 00.004 seconds)

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< 12 Uncompetitive Inhibitor Calculators

Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor

Go Enzyme Substrate Modifying Factor = ((Maximum Rate*Substrate Concentration)-(Initial Reaction Rate*Michaelis Constant))/(Initial Reaction Rate*Substrate Concentration)

Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant

Go Enzyme Substrate Complex Concentration = (Forward Rate Constant*Catalyst Concentration*Substrate Concentration)/(Reverse Rate Constant+Catalytic Rate Constant)

Initial Reaction Rate in presence of Uncompetitive Inhibitor

Go Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration))

Maximum Reaction Rate in Presence of Uncompetitive Inhibitor

Go Maximum Rate = (Initial Reaction Rate*(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration)))/Substrate Concentration

Substrate Concentration in presence of Uncompetitive Inhibitor

Go Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor))

Michaelis Constant in Presence of Uncompetitive Inhibitor

Go Michaelis Constant = (Substrate Concentration*(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor)))/Initial Reaction Rate

Enzyme Substrate Inhibitor Concentration in presence of Uncompetitive Inhibitor

Go Enzyme Substrate Inhibitor Complex Concentration = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Dissociation Constant

Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor

Go Enzyme Substrate Dissociation Constant = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Inhibitor Complex Concentration

Enzyme Substrate complex Concentration in Presence of Uncompetitive Inhibitor

Go Enzyme Substrate Complex Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Inhibitor Concentration

Inhibitor Concentration in Presence of Uncompetitive Inhibitor

Go Inhibitor Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Enzyme Substrate Complex Concentration

Enzyme Substrate Modifying Factor given Enzyme Substrate Dissociation Constant

Go Enzyme Substrate Modifying Factor = (1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant))

Enzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor

Go Enzyme Substrate Dissociation Constant = Inhibitor Concentration/(Enzyme Substrate Modifying Factor-1)

Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant Formula

Enzyme Substrate Complex Concentration = (Forward Rate Constant*Catalyst Concentration*Substrate Concentration)/(Reverse Rate Constant+Catalytic Rate Constant)
ES = (k_f*E*S)/(k_r+k_cat)

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant?

Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant calculator uses Enzyme Substrate Complex Concentration = (Forward Rate Constant*Catalyst Concentration*Substrate Concentration)/(Reverse Rate Constant+Catalytic Rate Constant) to calculate the Enzyme Substrate Complex Concentration, The Enzyme substate complex concentration given forward, reverse, and catalytic rate constants formula is defined as the relation with enzymatic catalyst and with substrate concentration in an enzymatic reaction mechanism. Enzyme Substrate Complex Concentration is denoted by ES symbol.

How to calculate Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant using this online calculator? To use this online calculator for Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant, enter Forward Rate Constant (k_f), Catalyst Concentration (E), Substrate Concentration (S), Reverse Rate Constant (k_r) & Catalytic Rate Constant (k_cat) and hit the calculate button. Here is how the Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant calculation can be explained with given input values -> 0.012937 = (6.9*25000*1500)/(20000+0.65).

FAQ

What is Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant?

The Enzyme substate complex concentration given forward, reverse, and catalytic rate constants formula is defined as the relation with enzymatic catalyst and with substrate concentration in an enzymatic reaction mechanism and is represented as ES = (k_f*E*S)/(k_r+k_cat) or Enzyme Substrate Complex Concentration = (Forward Rate Constant*Catalyst Concentration*Substrate Concentration)/(Reverse Rate Constant+Catalytic Rate Constant). The Forward Rate Constant is defined as the rate constant for the forward occurring reaction, The Catalyst Concentration is the number of moles of catalyst present in the per liter of solution, The Substrate Concentration is the number of moles of substrate per liter solution, The Reverse Rate Constant is defined as the rate constant for the backward reaction & The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.

How to calculate Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant?

The Enzyme substate complex concentration given forward, reverse, and catalytic rate constants formula is defined as the relation with enzymatic catalyst and with substrate concentration in an enzymatic reaction mechanism is calculated using Enzyme Substrate Complex Concentration = (Forward Rate Constant*Catalyst Concentration*Substrate Concentration)/(Reverse Rate Constant+Catalytic Rate Constant). To calculate Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant, you need Forward Rate Constant (k_f), Catalyst Concentration (E), Substrate Concentration (S), Reverse Rate Constant (k_r) & Catalytic Rate Constant (k_cat). With our tool, you need to enter the respective value for Forward Rate Constant, Catalyst Concentration, Substrate Concentration, Reverse Rate Constant & Catalytic Rate Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Enzyme Substrate Complex Concentration?

In this formula, Enzyme Substrate Complex Concentration uses Forward Rate Constant, Catalyst Concentration, Substrate Concentration, Reverse Rate Constant & Catalytic Rate Constant. We can use 1 other way(s) to calculate the same, which is/are as follows -

Enzyme Substrate Complex Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Inhibitor Concentration

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