Forward Rate Constant in Enzymatic Reaction mechanism Solution

STEP 0: Pre-Calculation Summary
Formula Used
Forward Rate Constant = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))
kf = (kr*ES)/(S*([E0]-ES))
This formula uses 5 Variables
Variables Used
Forward Rate Constant - (Measured in 1 Per Second) - The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.
Reverse Rate Constant - (Measured in Mole per Cubic Meter Second) - The Reverse Rate Constant is defined as the rate constant for the backward reaction.
Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
STEP 1: Convert Input(s) to Base Unit
Reverse Rate Constant: 20 Mole per Liter Second --> 20000 Mole per Cubic Meter Second (Check conversion here)
Enzyme Substrate Complex Concentration: 10 Mole per Liter --> 10000 Mole per Cubic Meter (Check conversion here)
Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion here)
Initial Enzyme Concentration: 100 Mole per Liter --> 100000 Mole per Cubic Meter (Check conversion here)
STEP 2: Evaluate Formula
Substituting Input Values in Formula
kf = (kr*ES)/(S*([E0]-ES)) --> (20000*10000)/(1500*(100000-10000))
Evaluating ... ...
kf = 1.48148148148148
STEP 3: Convert Result to Output's Unit
1.48148148148148 1 Per Second --> No Conversion Required
FINAL ANSWER
1.48148148148148 1.481481 1 Per Second <-- Forward Rate Constant
(Calculation completed in 00.004 seconds)

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16 Rate Constants of Enzymatic Reaction Calculators

Forward Rate Constant in Enzymatic Reaction mechanism
Go Forward Rate Constant = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))
Reverse Rate Constant in Enzymatic Reaction Mechanism
Go Reverse Rate Constant = (Forward Rate Constant*Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))/Enzyme Substrate Complex Concentration
Forward Rate Constant given Reverse and Catalytic Rate Constant
Go Forward Rate Constant = (Reverse Rate Constant+Catalytic Rate Constant)*(Enzyme Substrate Complex Concentration/(Catalyst Concentration*Substrate Concentration))
Reverse Rate Constant given Forward and Catalytic Rate Constants
Go Reverse Rate Constant = ((Forward Rate Constant*Catalyst Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Catalytic Rate Constant
Catalytic Rate Constant given Reverse and Forward Rate Constant
Go Catalytic Rate Constant = ((Forward Rate Constant*Catalyst Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Reverse Rate Constant
Catalytic Rate Constant given Dissociation Rate Constant
Go Catalytic Rate Constant = (Initial Reaction Rate*(Dissociation Rate Constant+Substrate Concentration))/(Initial Enzyme Concentration*Substrate Concentration)
Dissociation Rate Constant given Catalytic Rate Constant
Go Dissociation Rate Constant = ((Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration
Catalytic Rate Constant at Low Substrate Concentration
Go Catalytic Rate Constant = (Initial Reaction Rate*Michaelis Constant)/(Initial Enzyme Concentration*Substrate Concentration)
Dissociation Rate Constant given Concentration of Enzyme and Substrate
Go Dissociation Rate Constant = ((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration
Reverse Rate Constant given Michaelis Constant
Go Reverse Rate Constant = (Michaelis Constant*Forward Rate Constant)-Catalytic Rate Constant
Rate Constant given Initial Rate and Enzyme Substrate Complex Concentration
Go Final Rate Constant = Initial Reaction Rate/Enzyme Substrate Complex Concentration
Forward Rate Constant given Dissociation Rate Constant
Go Forward Rate Constant = (Reverse Rate Constant/Dissociation Rate Constant)
Reverse Rate Constant given Dissociation Rate Constant
Go Reverse Rate Constant = (Dissociation Rate Constant*Forward Rate Constant)
Dissociation Rate Constant in Enzymatic Reaction Mechanism
Go Dissociation Rate Constant = Reverse Rate Constant/Forward Rate Constant
Rate of Chemical Reaction
Go Rate of chemical reaction = Change in concentration/Total time interval
Rate Constant given Maximum Rate and Initial Enzyme Concentration
Go Final Rate Constant = Maximum Rate/Initial Enzyme Concentration

Forward Rate Constant in Enzymatic Reaction mechanism Formula

Forward Rate Constant = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))
kf = (kr*ES)/(S*([E0]-ES))

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Forward Rate Constant in Enzymatic Reaction mechanism?

Forward Rate Constant in Enzymatic Reaction mechanism calculator uses Forward Rate Constant = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)) to calculate the Forward Rate Constant, The Forward rate constant in enzymatic reaction mechanism formula is defined as the relation with the rate constant of backward reaction and with the concentration of enzyme and substrate. Forward Rate Constant is denoted by kf symbol.

How to calculate Forward Rate Constant in Enzymatic Reaction mechanism using this online calculator? To use this online calculator for Forward Rate Constant in Enzymatic Reaction mechanism, enter Reverse Rate Constant (kr), Enzyme Substrate Complex Concentration (ES), Substrate Concentration (S) & Initial Enzyme Concentration ([E0]) and hit the calculate button. Here is how the Forward Rate Constant in Enzymatic Reaction mechanism calculation can be explained with given input values -> 1.481481 = (20000*10000)/(1500*(100000-10000)).

FAQ

What is Forward Rate Constant in Enzymatic Reaction mechanism?
The Forward rate constant in enzymatic reaction mechanism formula is defined as the relation with the rate constant of backward reaction and with the concentration of enzyme and substrate and is represented as kf = (kr*ES)/(S*([E0]-ES)) or Forward Rate Constant = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)). The Reverse Rate Constant is defined as the rate constant for the backward reaction, The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate, The Substrate Concentration is the number of moles of substrate per liter solution & The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
How to calculate Forward Rate Constant in Enzymatic Reaction mechanism?
The Forward rate constant in enzymatic reaction mechanism formula is defined as the relation with the rate constant of backward reaction and with the concentration of enzyme and substrate is calculated using Forward Rate Constant = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Substrate Concentration*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)). To calculate Forward Rate Constant in Enzymatic Reaction mechanism, you need Reverse Rate Constant (kr), Enzyme Substrate Complex Concentration (ES), Substrate Concentration (S) & Initial Enzyme Concentration ([E0]). With our tool, you need to enter the respective value for Reverse Rate Constant, Enzyme Substrate Complex Concentration, Substrate Concentration & Initial Enzyme Concentration and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
How many ways are there to calculate Forward Rate Constant?
In this formula, Forward Rate Constant uses Reverse Rate Constant, Enzyme Substrate Complex Concentration, Substrate Concentration & Initial Enzyme Concentration. We can use 2 other way(s) to calculate the same, which is/are as follows -
  • Forward Rate Constant = (Reverse Rate Constant/Dissociation Rate Constant)
  • Forward Rate Constant = (Reverse Rate Constant+Catalytic Rate Constant)*(Enzyme Substrate Complex Concentration/(Catalyst Concentration*Substrate Concentration))
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