Modifying Factor of Enzyme in Michaelis Menten Equation Solution

STEP 0: Pre-Calculation Summary
Formula Used
Enzyme Modifying Factor = (((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-(Enzyme Substrate Modifying Factor*Substrate Concentration))/Michaelis Constant
α = (((Vmax*S)/V0)-(α'*S))/KM
This formula uses 6 Variables
Variables Used
Enzyme Modifying Factor - The Enzyme Modifying Factor is defined by the inhibitor concentration and the dissociation constants of enzyme.
Maximum Rate - (Measured in Mole per Cubic Meter Second) - The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Initial Reaction Rate - (Measured in Mole per Cubic Meter Second) - The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Enzyme Substrate Modifying Factor - The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.
Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
STEP 1: Convert Input(s) to Base Unit
Maximum Rate: 40 Mole per Liter Second --> 40000 Mole per Cubic Meter Second (Check conversion here)
Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion here)
Initial Reaction Rate: 0.45 Mole per Liter Second --> 450 Mole per Cubic Meter Second (Check conversion here)
Enzyme Substrate Modifying Factor: 2 --> No Conversion Required
Michaelis Constant: 3 Mole per Liter --> 3000 Mole per Cubic Meter (Check conversion here)
STEP 2: Evaluate Formula
Substituting Input Values in Formula
α = (((Vmax*S)/V0)-(α'*S))/KM --> (((40000*1500)/450)-(2*1500))/3000
Evaluating ... ...
α = 43.4444444444444
STEP 3: Convert Result to Output's Unit
43.4444444444444 --> No Conversion Required
FINAL ANSWER
43.4444444444444 43.44444 <-- Enzyme Modifying Factor
(Calculation completed in 00.004 seconds)

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25 Michaelis Menten Kinetics Equation Calculators

Michaelis Constant given Modifying Factor in Michaelis Menten Equation
Go Michaelis Constant = (Substrate Concentration*((1/Enzyme Substrate Modifying Factor)*Maximum Rate)-Initial Reaction Rate)/((Enzyme Modifying Factor/Enzyme Substrate Modifying Factor)*Substrate Concentration)
Initial Reaction Rate of Enzyme given Modifying factor in Michaelis Menten equation
Go Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/((Enzyme Modifying Factor*Michaelis Constant)+(Enzyme Substrate Modifying Factor*Substrate Concentration))
Modifying Factor of Enzyme Substrate Complex in Michaelis Menten Equation
Go Enzyme Substrate Modifying Factor = (((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-(Enzyme Modifying Factor*Michaelis Constant))/Substrate Concentration
Maximum Rate given Modifying Factor in Michaelis Menten Equation
Go Maximum Rate = (Initial Reaction Rate*((Enzyme Modifying Factor*Michaelis Constant)+(Enzyme Substrate Modifying Factor*Substrate Concentration)))/Substrate Concentration
Modifying Factor of Enzyme in Michaelis Menten Equation
Go Enzyme Modifying Factor = (((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-(Enzyme Substrate Modifying Factor*Substrate Concentration))/Michaelis Constant
Catalytic Rate Constant from Michaelis Menten Kinetics Equation
Go Catalytic Rate Constant for MM = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/(Initial Enzyme Concentration*Substrate Concentration)
Enzyme Concentration from Michaelis Menten Kinetics equation
Go Initial Concentration of Enzyme = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/(Catalytic Rate Constant*Substrate Concentration)
Michaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration
Go Michaelis Constant = (Substrate Concentration*((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate))/Initial Reaction Rate
Michaelis Constant at Low Substrate Concentration
Go Michaelis Constant = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate
Dissociation Rate Constant from Michaelis Menten kinetics equation
Go Dissociation Rate Constant = ((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-(Substrate Concentration)
Initial Rate given Apparent value of Michaelis Menten Constant
Go Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Apparent Michaelis Constant+Substrate Concentration)
Maximum Rate given Apparent Value of Michaelis Menten Constant
Go Maximum Rate = (Initial Reaction Rate*(Apparent Michaelis Constant+Substrate Concentration))/Substrate Concentration
Maximum Rate of System from Michaelis Menten Kinetics equation
Go Maximum Rate = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/Substrate Concentration
Substrate Concentration from Michaelis Menten Kinetics Equation
Go Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)
Michaelis Constant from Michaelis Menten kinetics equation
Go Michaelis Constant = Substrate Concentration*((Maximum Rate-Initial Reaction Rate)/Initial Reaction Rate)
Inhibitor's Dissociation Constant given Michaelis Menten Constant
Go Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Apparent Michaelis Constant/Michaelis Constant)-1))
Michaelis Menten constant given Apparent Michaelis Menten Constant
Go Michaelis Constant = Apparent Michaelis Constant/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))
Inhibitor Concentration given Apparent Michaelis Menten Constant
Go Inhibitor Concentration = ((Apparent Michaelis Constant/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant
Michaelis Constant given Forward, Reverse, and Catalytic Rate Constants
Go Michaelis Constant = (Reverse Rate Constant+Catalytic Rate Constant)/Forward Rate Constant
Catalytic rate constant given Michaelis Constant
Go Catalytic Rate Constant = (Michaelis Constant*Forward Rate Constant)-Reverse Rate Constant
Forward Rate Constant given Michaelis Constant
Go Forward Rate Constant = (Reverse Rate Constant+Catalytic Rate Constant)/Michaelis Constant
Michaelis Constant given Maximum Rate at Low Substrate Concentration
Go Michaelis Constant = (Maximum Rate*Substrate Concentration)/Initial Reaction Rate
Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant
Go Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant
Catalytic Rate Constant if Substrate Concentration is higher than Michaelis Constant
Go Catalytic Rate Constant = Maximum Rate/Initial Enzyme Concentration
Maximum Rate if Substrate Concentration is Higher than Michaelis Constant
Go Maximum Rate = Catalytic Rate Constant*Initial Enzyme Concentration

Modifying Factor of Enzyme in Michaelis Menten Equation Formula

Enzyme Modifying Factor = (((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-(Enzyme Substrate Modifying Factor*Substrate Concentration))/Michaelis Constant
α = (((Vmax*S)/V0)-(α'*S))/KM

What is competitive Inhibition?

In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time, as shown in the figure on the right. This usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site. This type of inhibition can be overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.

How to Calculate Modifying Factor of Enzyme in Michaelis Menten Equation?

Modifying Factor of Enzyme in Michaelis Menten Equation calculator uses Enzyme Modifying Factor = (((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-(Enzyme Substrate Modifying Factor*Substrate Concentration))/Michaelis Constant to calculate the Enzyme Modifying Factor, The Modifying factor of enzyme in Michaelis Menten equation formula is defined as the enzyme activity under various substrate and inhibitor concentrations, to obtain a modified Michaelis–Menten equation. Enzyme Modifying Factor is denoted by α symbol.

How to calculate Modifying Factor of Enzyme in Michaelis Menten Equation using this online calculator? To use this online calculator for Modifying Factor of Enzyme in Michaelis Menten Equation, enter Maximum Rate (Vmax), Substrate Concentration (S), Initial Reaction Rate (V0), Enzyme Substrate Modifying Factor ') & Michaelis Constant (KM) and hit the calculate button. Here is how the Modifying Factor of Enzyme in Michaelis Menten Equation calculation can be explained with given input values -> 43.44444 = (((40000*1500)/450)-(2*1500))/3000.

FAQ

What is Modifying Factor of Enzyme in Michaelis Menten Equation?
The Modifying factor of enzyme in Michaelis Menten equation formula is defined as the enzyme activity under various substrate and inhibitor concentrations, to obtain a modified Michaelis–Menten equation and is represented as α = (((Vmax*S)/V0)-(α'*S))/KM or Enzyme Modifying Factor = (((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-(Enzyme Substrate Modifying Factor*Substrate Concentration))/Michaelis Constant. The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration, The Substrate Concentration is the number of moles of substrate per liter solution, The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place, The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex & The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
How to calculate Modifying Factor of Enzyme in Michaelis Menten Equation?
The Modifying factor of enzyme in Michaelis Menten equation formula is defined as the enzyme activity under various substrate and inhibitor concentrations, to obtain a modified Michaelis–Menten equation is calculated using Enzyme Modifying Factor = (((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-(Enzyme Substrate Modifying Factor*Substrate Concentration))/Michaelis Constant. To calculate Modifying Factor of Enzyme in Michaelis Menten Equation, you need Maximum Rate (Vmax), Substrate Concentration (S), Initial Reaction Rate (V0), Enzyme Substrate Modifying Factor ') & Michaelis Constant (KM). With our tool, you need to enter the respective value for Maximum Rate, Substrate Concentration, Initial Reaction Rate, Enzyme Substrate Modifying Factor & Michaelis Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
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