Modifying Factor of Enzyme Substrate Complex Calculator

✖The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.ⓘ Inhibitor Concentration [I]			+10% -10%
✖The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.ⓘ Enzyme Substrate Dissociation Constant [K_i']			+10% -10%

✖The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.ⓘ Modifying Factor of Enzyme Substrate Complex [α^']

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Modifying Factor of Enzyme Substrate Complex

Formula

`"α"^{"'"} = 1+("I"/("K"_{"i"}"'"))`

Example

`"1.6"=1+("9mol/L"/"15mol/L")`

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Modifying Factor of Enzyme Substrate Complex Solution

STEP 0: Pre-Calculation Summary

Formula Used

Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant)
α^' = 1+(I/K_i')
This formula uses 3 Variables

Variables Used

Enzyme Substrate Modifying Factor - The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.
Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Enzyme Substrate Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.

STEP 1: Convert Input(s) to Base Unit

Inhibitor Concentration: 9 Mole per Liter --> 9000 Mole per Cubic Meter (Check conversion here)
Enzyme Substrate Dissociation Constant: 15 Mole per Liter --> 15000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

α^' = 1+(I/K_i') --> 1+(9000/15000)

Evaluating ... ...

α^' = 1.6

STEP 3: Convert Result to Output's Unit

1.6 --> No Conversion Required

FINAL ANSWER

1.6 <-- Enzyme Substrate Modifying Factor

(Calculation completed in 00.004 seconds)

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< 13 Enzyme Kinetics Calculators

Initial Reaction Rate given Catalytic Rate Constant and Dissociation Rate Constants

Go Initial Reaction Rate = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)

Initial Reaction Rate given Catalytic Rate Constant and Initial Enzyme Concentration

Go Initial Reaction Rate = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/(Michaelis Constant+Substrate Concentration)

Initial Reaction Rate given Dissociation Rate Constant

Go Initial Reaction Rate given DRC = (Maximum Rate*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)

Maximum Rate given Dissociation Rate Constant

Go Maximum Rate given DRC = (Initial Reaction Rate*(Dissociation Rate Constant+Substrate Concentration))/Substrate Concentration

Initial Reaction Rate at Low Substrate Concentration

Go Initial Reaction Rate = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Michaelis Constant

Initial Reaction Rate in Michaelis Menten kinetics Equation

Go Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Michaelis Constant+Substrate Concentration)

Initial Reaction Rate at Low Substrate Concentration terms of Maximum Rate

Go Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/Michaelis Constant

Maximum Rate of System at Low Substrate Concentration

Go Maximum Rate = (Initial Reaction Rate*Michaelis Constant)/Substrate Concentration

Modifying Factor of Enzyme Substrate Complex

Go Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant)

Initial Rate of System given Rate Constant and Enzyme Substrate Complex Concentration

Go Initial Reaction Rate given RC = Final Rate Constant*Enzyme Substrate Complex Concentration

Maximum Rate given Rate Constant and Initial Enzyme Concentration

Go Maximum Rate = (Final Rate Constant*Initial Enzyme Concentration)

Total Change in Concentration of Reaction

Go Total change in concentration = Average rate*Total time interval

Total Time taken during Reaction

Go Total time interval = Total change in concentration/Average rate

< 25 Important Formulas on Enzyme Kinetics Calculators

Final Rate Constant for Competitive Inhibition of Enzyme Catalysis

Go Final Rate Constant for Catalysis = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Initial Enzyme Concentration*Substrate Concentration)

Inhibitor Concentration for Competitive Inhibition of Enzyme Catalysis

Go Inhibitor Concentration given IEC = (((((Final Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant

Michaelis Constant in Competitive Inhibition given Enzyme Substrate Complex Concentration

Go Michaelis Constant = (((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))

Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis

Go Enzyme Substrate Complex Concentration = (Substrate Concentration*Initial Enzyme Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration)

Inhibitor Concentration in Competitive Inhibition given Maximum Rate of System

Go Inhibitor Concentration given Max Rate = (((((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant

Initial Rate in Competitive Inhibition given Maximum Rate of system

Go Initial Reaction Rate in CI = (Maximum Rate*Substrate Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration)

Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants

Go Catalyst Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration)

Catalytic Rate Constant from Michaelis Menten Kinetics Equation

Go Catalytic Rate Constant for MM = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/(Initial Enzyme Concentration*Substrate Concentration)

Enzyme Concentration from Michaelis Menten Kinetics equation

Go Initial Concentration of Enzyme = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/(Catalytic Rate Constant*Substrate Concentration)

Substrate Concentration given Catalytic Rate Constant and Initial Enzyme Concentration

Go Concentration of Substrate = (Michaelis Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Initial Enzyme Concentration given Dissociation Rate Constant

Go Enzyme Concentration Initially = (Enzyme Substrate Complex Concentration*(Dissociation Rate Constant+Substrate Concentration))/(Substrate Concentration)

Initial Reaction Rate given Dissociation Rate Constant

Go Initial Reaction Rate given DRC = (Maximum Rate*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)

Maximum Rate given Dissociation Rate Constant

Go Maximum Rate given DRC = (Initial Reaction Rate*(Dissociation Rate Constant+Substrate Concentration))/Substrate Concentration

Inhibitor Concentration given Apparent Initial Enzyme Concentration

Go Inhibitor Concentration for CI = ((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1)*Enzyme Inhibitor Dissociation Constant

Initial Concentration of Enzyme in presence of Inhibitor by Enzyme Conservation Law

Go Enzyme Concentration Initially = (Catalyst Concentration+Enzyme Substrate Complex Concentration+Enzyme Inhibitor Complex Concentration)

Michaelis Constant given Forward, Reverse, and Catalytic Rate Constants

Go Michaelis Constant = (Reverse Rate Constant+Catalytic Rate Constant)/Forward Rate Constant

Inhibitor Concentration given Enzyme Substrate Modifying Factor

Go Inhibitor Concentration = (Enzyme Substrate Modifying Factor-1)*Enzyme Substrate Dissociation Constant

Modifying Factor of Enzyme Substrate Complex

Go Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant)

Dissociation Constant of Enzyme given Modifying Factor of Enzyme

Go Enzyme Inhibitor Dissociation Constant given MF = Inhibitor Concentration/(Enzyme Modifying Factor-1)

Initial Rate of System given Rate Constant and Enzyme Substrate Complex Concentration

Go Initial Reaction Rate given RC = Final Rate Constant*Enzyme Substrate Complex Concentration

Forward Rate Constant given Dissociation Rate Constant

Go Forward Rate Constant = (Reverse Rate Constant/Dissociation Rate Constant)

Dissociation Rate Constant in Enzymatic Reaction Mechanism

Go Dissociation Rate Constant = Reverse Rate Constant/Forward Rate Constant

Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant

Go Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant

Catalytic Rate Constant if Substrate Concentration is higher than Michaelis Constant

Go Catalytic Rate Constant = Maximum Rate/Initial Enzyme Concentration

Maximum Rate if Substrate Concentration is Higher than Michaelis Constant

Go Maximum Rate = Catalytic Rate Constant*Initial Enzyme Concentration

Modifying Factor of Enzyme Substrate Complex Formula

Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant)
α^' = 1+(I/K_i')

What are the modifying factor of enzyme?

Factors such as pH, temperature, effectors, and inhibitors modify the enzyme conformation, altering its catalytic activity.

What is competitive Inhibition?

In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time, as shown in the figure on the right. This usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site. This type of inhibition can be overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.

How to Calculate Modifying Factor of Enzyme Substrate Complex?

Modifying Factor of Enzyme Substrate Complex calculator uses Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant) to calculate the Enzyme Substrate Modifying Factor, The Modifying factor of enzyme substrate complex formula is defined as the relation with inhibitor concentration and with the enzyme-substrate dissociation constant. Enzyme Substrate Modifying Factor is denoted by α^' symbol.

How to calculate Modifying Factor of Enzyme Substrate Complex using this online calculator? To use this online calculator for Modifying Factor of Enzyme Substrate Complex, enter Inhibitor Concentration (I) & Enzyme Substrate Dissociation Constant (K_i') and hit the calculate button. Here is how the Modifying Factor of Enzyme Substrate Complex calculation can be explained with given input values -> 1.6 = 1+(9000/15000).

FAQ

What is Modifying Factor of Enzyme Substrate Complex?

The Modifying factor of enzyme substrate complex formula is defined as the relation with inhibitor concentration and with the enzyme-substrate dissociation constant and is represented as α^' = 1+(I/K_i') or Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant). The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system & The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.

How to calculate Modifying Factor of Enzyme Substrate Complex?

The Modifying factor of enzyme substrate complex formula is defined as the relation with inhibitor concentration and with the enzyme-substrate dissociation constant is calculated using Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant). To calculate Modifying Factor of Enzyme Substrate Complex, you need Inhibitor Concentration (I) & Enzyme Substrate Dissociation Constant (K_i'). With our tool, you need to enter the respective value for Inhibitor Concentration & Enzyme Substrate Dissociation Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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