Relative Retention given Adjusted Retention Times Calculator

✖The Adjusted Retention Time of Solute 2 is the removal of time taken by the mobile phase to travel the column from the retention time of solute 2.ⓘ Adjusted Retention Time of Solute 2 [tr2^']			+10% -10%
✖The Adjusted Retention Time of Solute 1 is the removal of time taken by the mobile phase to travel the column from the retention time of solute 1.ⓘ Adjusted Retention Time of Solute 1 [tr1^']			+10% -10%

✖Actual Relative Retention is the ratio of adjusted retention times for any two components.ⓘ Relative Retention given Adjusted Retention Times [α_R]

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Formula

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Relative Retention given Adjusted Retention Times

Formula

`"α"_{"R"} = ("tr2"^{"'"}/"tr1"^{"'"})`

Example

`"2"=("10s"/"5s")`

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Relative Retention given Adjusted Retention Times Solution

STEP 0: Pre-Calculation Summary

Formula Used

Actual Relative Retention = (Adjusted Retention Time of Solute 2/Adjusted Retention Time of Solute 1)
α_R = (tr2^'/tr1^')
This formula uses 3 Variables

Variables Used

Actual Relative Retention - Actual Relative Retention is the ratio of adjusted retention times for any two components.
Adjusted Retention Time of Solute 2 - (Measured in Second) - The Adjusted Retention Time of Solute 2 is the removal of time taken by the mobile phase to travel the column from the retention time of solute 2.
Adjusted Retention Time of Solute 1 - (Measured in Second) - The Adjusted Retention Time of Solute 1 is the removal of time taken by the mobile phase to travel the column from the retention time of solute 1.

STEP 1: Convert Input(s) to Base Unit

Adjusted Retention Time of Solute 2: 10 Second --> 10 Second No Conversion Required
Adjusted Retention Time of Solute 1: 5 Second --> 5 Second No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

α_R = (tr2^'/tr1^') --> (10/5)

Evaluating ... ...

α_R = 2

STEP 3: Convert Result to Output's Unit

2 --> No Conversion Required

FINAL ANSWER

2 <-- Actual Relative Retention

(Calculation completed in 00.004 seconds)

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Created by Prashant Singh

K J Somaiya College of science (K J Somaiya), Mumbai

Prashant Singh has created this Calculator and 700+ more calculators!

Verified by Akshada Kulkarni

National Institute of Information Technology (NIIT), Neemrana

Akshada Kulkarni has verified this Calculator and 900+ more calculators!

< 7 Relative and Adjusted Retention Calculators

Relative Retention given Adjusted Retention Times

Go Actual Relative Retention = (Adjusted Retention Time of Solute 2/Adjusted Retention Time of Solute 1)

Relative Retention given Partition Coefficient of Two Components

Go Actual Relative Retention = (Partition Coefficient of Solute 2/Partition Coefficient of Solute 1)

Adjusted Retention of Second Component given Relative Retention

Go Adjusted Retention Time of Comp 2 = (Relative Retention*Adjusted Retention Time of Solute 1)

Adjusted Retention of First Component given Relative Retention

Go Adjusted Retention Time of Comp 1 = (Adjusted Retention Time of Solute 2/Relative Retention)

Partition Coefficient of Solute 1 given Relative Retention

Go Partition Coefficient of Comp 1 = (Partition Coefficient of Solute 2/Relative Retention)

Partition Coefficient of Solute 2 given Relative Retention

Go Partition Coefficient of Comp 2 = (Relative Retention*Partition Coefficient of Solute 1)

Relative Retention given Capacity Factor of Two Components

Go Actual Relative Retention = (Capacity Factor of Solute 2/Capacity Factor of Solute 1)

< 13 Relative and Adjusted Retention and Phase Calculators

Molar Concentration of Third Component in Second Phase

Go Concentration of Solute in Phase2 = (Concentration of Solute in Solvent 1/Distribution Coefficient of Solution)

Molar Concentration of Third Component in First Phase

Go Concentration of Solute in Phase1 = (Distribution Coefficient of Solution*Solute Concentration in Solvent2)

Relative Retention given Adjusted Retention Times

Go Actual Relative Retention = (Adjusted Retention Time of Solute 2/Adjusted Retention Time of Solute 1)

Relative Retention given Partition Coefficient of Two Components

Go Actual Relative Retention = (Partition Coefficient of Solute 2/Partition Coefficient of Solute 1)

Adjusted Retention of Second Component given Relative Retention

Go Adjusted Retention Time of Comp 2 = (Relative Retention*Adjusted Retention Time of Solute 1)

Adjusted Retention of First Component given Relative Retention

Go Adjusted Retention Time of Comp 1 = (Adjusted Retention Time of Solute 2/Relative Retention)

Partition Coefficient of Solute 1 given Relative Retention

Go Partition Coefficient of Comp 1 = (Partition Coefficient of Solute 2/Relative Retention)

Partition Coefficient of Solute 2 given Relative Retention

Go Partition Coefficient of Comp 2 = (Relative Retention*Partition Coefficient of Solute 1)

Mobile Phase Travel Time through Column

Go Unretained Solute Travel Time through Column = (Retention Time-Adjusted Retention Time)

Total Concentration of Solute in Aqueous Phase

Go Concentration in Aqueous Solvent = (Concentration in Organic Phase/Distribution Ratio)

Total Concentration of Solute in Organic Phase

Go Concentration in Organic Solvent = (Distribution Ratio*Concentration in Aqueous Phase)

Relative Retention given Capacity Factor of Two Components

Go Actual Relative Retention = (Capacity Factor of Solute 2/Capacity Factor of Solute 1)

Mobile Phase Travel Time given Capacity Factor

Go Unretained Solute Travel Time given CP = (Retention Time)/(Capacity Factor+1)

Relative Retention given Adjusted Retention Times Formula

Actual Relative Retention = (Adjusted Retention Time of Solute 2/Adjusted Retention Time of Solute 1)
α_R = (tr2^'/tr1^')

What is Chromatography?

A separation process based on the various partitioning coefficients of different solutes between the two phases.
Involving the interaction of solute(s) and two phases
Mobile phase: A gas or liquid that moves through the column.
Stationary phase: A solid or liquid that remains in place.

What are the types of Chromatography?

1) Adsorption chromatography
2) Ion-exchange chromatography
3) Partition chromatography
4) Molecular Size exclusion chromatography
5) Affinity chromatography

How to Calculate Relative Retention given Adjusted Retention Times?

Relative Retention given Adjusted Retention Times calculator uses Actual Relative Retention = (Adjusted Retention Time of Solute 2/Adjusted Retention Time of Solute 1) to calculate the Actual Relative Retention, The Relative retention given adjusted retention times formula is defined as the ratio of adjusted retention times for any two components. The greater the relative retention, the greater the separation. Actual Relative Retention is denoted by α_R symbol.

How to calculate Relative Retention given Adjusted Retention Times using this online calculator? To use this online calculator for Relative Retention given Adjusted Retention Times, enter Adjusted Retention Time of Solute 2 (tr2^') & Adjusted Retention Time of Solute 1 (tr1^') and hit the calculate button. Here is how the Relative Retention given Adjusted Retention Times calculation can be explained with given input values -> 2 = (10/5).

FAQ

What is Relative Retention given Adjusted Retention Times?

The Relative retention given adjusted retention times formula is defined as the ratio of adjusted retention times for any two components. The greater the relative retention, the greater the separation and is represented as α_R = (tr2^'/tr1^') or Actual Relative Retention = (Adjusted Retention Time of Solute 2/Adjusted Retention Time of Solute 1). The Adjusted Retention Time of Solute 2 is the removal of time taken by the mobile phase to travel the column from the retention time of solute 2 & The Adjusted Retention Time of Solute 1 is the removal of time taken by the mobile phase to travel the column from the retention time of solute 1.

How to calculate Relative Retention given Adjusted Retention Times?

The Relative retention given adjusted retention times formula is defined as the ratio of adjusted retention times for any two components. The greater the relative retention, the greater the separation is calculated using Actual Relative Retention = (Adjusted Retention Time of Solute 2/Adjusted Retention Time of Solute 1). To calculate Relative Retention given Adjusted Retention Times, you need Adjusted Retention Time of Solute 2 (tr2^') & Adjusted Retention Time of Solute 1 (tr1^'). With our tool, you need to enter the respective value for Adjusted Retention Time of Solute 2 & Adjusted Retention Time of Solute 1 and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Actual Relative Retention?

In this formula, Actual Relative Retention uses Adjusted Retention Time of Solute 2 & Adjusted Retention Time of Solute 1. We can use 4 other way(s) to calculate the same, which is/are as follows -

Actual Relative Retention = (Capacity Factor of Solute 2/Capacity Factor of Solute 1)
Actual Relative Retention = (Partition Coefficient of Solute 2/Partition Coefficient of Solute 1)
Actual Relative Retention = (Capacity Factor of Solute 2/Capacity Factor of Solute 1)
Actual Relative Retention = (Partition Coefficient of Solute 2/Partition Coefficient of Solute 1)

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