Substrate Concentration in presence of Uncompetitive Inhibitor Calculator

✖The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.ⓘ Michaelis Constant [K_M]			+10% -10%
✖The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.ⓘ Initial Reaction Rate [V₀]			+10% -10%
✖The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.ⓘ Maximum Rate [V_max]			+10% -10%
✖The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.ⓘ Enzyme Substrate Modifying Factor [α^']			+10% -10%

✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration in presence of Uncompetitive Inhibitor [S]

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Formula

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Substrate Concentration in presence of Uncompetitive Inhibitor

Formula

`"S" = ("K"_{"M"}*"V"_{"0"})/("V"_{"max"}-("V"_{"0"}*"α"^{"'"}))`

Example

`"0.034527mol/L"=("3mol/L"*"0.45mol/L*s")/("40mol/L*s"-("0.45mol/L*s"*"2"))`

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Substrate Concentration in presence of Uncompetitive Inhibitor Solution

STEP 0: Pre-Calculation Summary

Formula Used

Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor))
S = (K_M*V₀)/(V_max-(V₀*α^'))
This formula uses 5 Variables

Variables Used

Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Initial Reaction Rate - (Measured in Mole per Cubic Meter Second) - The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Maximum Rate - (Measured in Mole per Cubic Meter Second) - The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.
Enzyme Substrate Modifying Factor - The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.

STEP 1: Convert Input(s) to Base Unit

Michaelis Constant: 3 Mole per Liter --> 3000 Mole per Cubic Meter (Check conversion here)
Initial Reaction Rate: 0.45 Mole per Liter Second --> 450 Mole per Cubic Meter Second (Check conversion here)
Maximum Rate: 40 Mole per Liter Second --> 40000 Mole per Cubic Meter Second (Check conversion here)
Enzyme Substrate Modifying Factor: 2 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

S = (K_M*V₀)/(V_max-(V₀*α^')) --> (3000*450)/(40000-(450*2))

Evaluating ... ...

S = 34.5268542199489

STEP 3: Convert Result to Output's Unit

34.5268542199489 Mole per Cubic Meter -->0.0345268542199489 Mole per Liter (Check conversion here)

FINAL ANSWER

0.0345268542199489 ≈ 0.034527 Mole per Liter <-- Substrate Concentration

(Calculation completed in 00.020 seconds)

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< 12 Uncompetitive Inhibitor Calculators

Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor

Go Enzyme Substrate Modifying Factor = ((Maximum Rate*Substrate Concentration)-(Initial Reaction Rate*Michaelis Constant))/(Initial Reaction Rate*Substrate Concentration)

Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant

Go Enzyme Substrate Complex Concentration = (Forward Rate Constant*Catalyst Concentration*Substrate Concentration)/(Reverse Rate Constant+Catalytic Rate Constant)

Initial Reaction Rate in presence of Uncompetitive Inhibitor

Go Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration))

Maximum Reaction Rate in Presence of Uncompetitive Inhibitor

Go Maximum Rate = (Initial Reaction Rate*(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration)))/Substrate Concentration

Substrate Concentration in presence of Uncompetitive Inhibitor

Go Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor))

Michaelis Constant in Presence of Uncompetitive Inhibitor

Go Michaelis Constant = (Substrate Concentration*(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor)))/Initial Reaction Rate

Enzyme Substrate Inhibitor Concentration in presence of Uncompetitive Inhibitor

Go Enzyme Substrate Inhibitor Complex Concentration = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Dissociation Constant

Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor

Go Enzyme Substrate Dissociation Constant = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Inhibitor Complex Concentration

Enzyme Substrate complex Concentration in Presence of Uncompetitive Inhibitor

Go Enzyme Substrate Complex Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Inhibitor Concentration

Inhibitor Concentration in Presence of Uncompetitive Inhibitor

Go Inhibitor Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Enzyme Substrate Complex Concentration

Enzyme Substrate Modifying Factor given Enzyme Substrate Dissociation Constant

Go Enzyme Substrate Modifying Factor = (1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant))

Enzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor

Go Enzyme Substrate Dissociation Constant = Inhibitor Concentration/(Enzyme Substrate Modifying Factor-1)

Substrate Concentration in presence of Uncompetitive Inhibitor Formula

Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor))
S = (K_M*V₀)/(V_max-(V₀*α^'))

What is Uncompetitive inhibitor?

Uncompetitive inhibition, also known as anti-competitive inhibition, takes place when an enzyme inhibitor binds only to the complex formed between the enzyme and the substrate (the E-S complex). Uncompetitive inhibition typically occurs in reactions with two or more substrates or products. Uncompetitive inhibition is distinguished from competitive inhibition by two observations: first uncompetitive inhibition cannot be reversed by increasing [S] and second, as shown, the Lineweaver–Burk plot yields parallel rather than intersecting lines.

How to Calculate Substrate Concentration in presence of Uncompetitive Inhibitor?

Substrate Concentration in presence of Uncompetitive Inhibitor calculator uses Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor)) to calculate the Substrate Concentration, The Substrate concentration in presence of uncompetitive inhibitor formula is defined by the altered Michaelis-Menten equation. Substrate Concentration is denoted by S symbol.

How to calculate Substrate Concentration in presence of Uncompetitive Inhibitor using this online calculator? To use this online calculator for Substrate Concentration in presence of Uncompetitive Inhibitor, enter Michaelis Constant (K_M), Initial Reaction Rate (V₀), Maximum Rate (V_max) & Enzyme Substrate Modifying Factor (α^') and hit the calculate button. Here is how the Substrate Concentration in presence of Uncompetitive Inhibitor calculation can be explained with given input values -> 3.5E-5 = (3000*450)/(40000-(450*2)).

FAQ

What is Substrate Concentration in presence of Uncompetitive Inhibitor?

The Substrate concentration in presence of uncompetitive inhibitor formula is defined by the altered Michaelis-Menten equation and is represented as S = (K_M*V₀)/(V_max-(V₀*α^')) or Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor)). The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system, The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place, The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration & The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.

How to calculate Substrate Concentration in presence of Uncompetitive Inhibitor?

The Substrate concentration in presence of uncompetitive inhibitor formula is defined by the altered Michaelis-Menten equation is calculated using Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor)). To calculate Substrate Concentration in presence of Uncompetitive Inhibitor, you need Michaelis Constant (K_M), Initial Reaction Rate (V₀), Maximum Rate (V_max) & Enzyme Substrate Modifying Factor (α^'). With our tool, you need to enter the respective value for Michaelis Constant, Initial Reaction Rate, Maximum Rate & Enzyme Substrate Modifying Factor and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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