Total Volume of Mobile Phase within Column given Retention Factor Calculator

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✖The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column.ⓘ Retention Volume [V_R]			+10% -10%
✖Retention Factor is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front.ⓘ Retention Factor [R_f]			+10% -10%

✖Void Volume is the volume of mobile phase in a column.ⓘ Total Volume of Mobile Phase within Column given Retention Factor [V₀]

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Formula

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Total Volume of Mobile Phase within Column given Retention Factor

Formula

`"V"_{"0"} = "V"_{"R"}/("R"_{"f"}+1)`

Example

`"1018.182mL"="11.2L"/("10"+1)`

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Total Volume of Mobile Phase within Column given Retention Factor Solution

STEP 0: Pre-Calculation Summary

Formula Used

Void Volume = Retention Volume/(Retention Factor+1)
V₀ = V_R/(R_f+1)
This formula uses 3 Variables

Variables Used

Void Volume - (Measured in Cubic Meter) - Void Volume is the volume of mobile phase in a column.
Retention Volume - (Measured in Cubic Meter) - The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column.
Retention Factor - Retention Factor is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front.

STEP 1: Convert Input(s) to Base Unit

Retention Volume: 11.2 Liter --> 0.0112 Cubic Meter (Check conversion here)
Retention Factor: 10 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

V₀ = V_R/(R_f+1) --> 0.0112/(10+1)

Evaluating ... ...

V₀ = 0.00101818181818182

STEP 3: Convert Result to Output's Unit

0.00101818181818182 Cubic Meter -->1018.18181818182 Milliliter (Check conversion here)

FINAL ANSWER

1018.18181818182 ≈ 1018.182 Milliliter <-- Void Volume

(Calculation completed in 00.004 seconds)

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National University of Judicial Science (NUJS), Kolkata

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< 17 Osmolality Calculators

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Total Volume of Mobile Phase within Column given Retention Factor Formula

Void Volume = Retention Volume/(Retention Factor+1)
V₀ = V_R/(R_f+1)

What is Column Chromatography?

Column chromatography separates substances based on differential adsorption of compounds to the adsorbent as the compounds move through the column at different rates which allow them to get separated in fractions. This technique can be used on a small scale as well as large scale to purify materials that can be used in future experiments. This method is a type of adsorption chromatography technique.

How to Calculate Total Volume of Mobile Phase within Column given Retention Factor?

Total Volume of Mobile Phase within Column given Retention Factor calculator uses Void Volume = Retention Volume/(Retention Factor+1) to calculate the Void Volume, The Total Volume of Mobile Phase within Column given Retention Factor formula in column chromatography is defined as the volume of the mobile phase is the total bed volume of the column minus the volume occupied by the support particles. Void Volume is denoted by V₀ symbol.

How to calculate Total Volume of Mobile Phase within Column given Retention Factor using this online calculator? To use this online calculator for Total Volume of Mobile Phase within Column given Retention Factor, enter Retention Volume (V_R) & Retention Factor (R_f) and hit the calculate button. Here is how the Total Volume of Mobile Phase within Column given Retention Factor calculation can be explained with given input values -> 1E+9 = 0.0112/(10+1).

FAQ

What is Total Volume of Mobile Phase within Column given Retention Factor?

The Total Volume of Mobile Phase within Column given Retention Factor formula in column chromatography is defined as the volume of the mobile phase is the total bed volume of the column minus the volume occupied by the support particles and is represented as V₀ = V_R/(R_f+1) or Void Volume = Retention Volume/(Retention Factor+1). The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column & Retention Factor is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front.

How to calculate Total Volume of Mobile Phase within Column given Retention Factor?

The Total Volume of Mobile Phase within Column given Retention Factor formula in column chromatography is defined as the volume of the mobile phase is the total bed volume of the column minus the volume occupied by the support particles is calculated using Void Volume = Retention Volume/(Retention Factor+1). To calculate Total Volume of Mobile Phase within Column given Retention Factor, you need Retention Volume (V_R) & Retention Factor (R_f). With our tool, you need to enter the respective value for Retention Volume & Retention Factor and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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