Volume of Stationary Phase given Capacity Factor and Partition Coefficient Calculator

✖The Capacity factor is directly proportional to the retention factor. The longer a component is retained by the column, the greater is the capacity factor.ⓘ Capacity Factor [k^']			+10% -10%
✖The Volume of Mobile Phase is the amount of the solvent that runs through the chromatography column.ⓘ Volume of Mobile Phase [V_{mobile phase}]			+10% -10%
✖The Partition Coefficient is defined as the ratio of the equilibrium concentrations of a dissolved substance in a two-phase system consisting of two largely immiscible solvents.ⓘ Partition Coefficient [K]			+10% -10%

✖The Volume of Stationary Phase is the amount of motionless part of the chromatography column.ⓘ Volume of Stationary Phase given Capacity Factor and Partition Coefficient [V_s]

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Formula

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Volume of Stationary Phase given Capacity Factor and Partition Coefficient

Formula

`"V"_{"s"} = ("k"^{"'"}*"V"_{"mobile phase"})/"K"`

Example

`"0.375L"=("3"*"5L")/"40"`

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Volume of Stationary Phase given Capacity Factor and Partition Coefficient Solution

STEP 0: Pre-Calculation Summary

Formula Used

Volume of Stationary Phase = (Capacity Factor*Volume of Mobile Phase)/Partition Coefficient
V_s = (k^'*V_{mobile phase})/K
This formula uses 4 Variables

Variables Used

Volume of Stationary Phase - (Measured in Cubic Meter) - The Volume of Stationary Phase is the amount of motionless part of the chromatography column.
Capacity Factor - The Capacity factor is directly proportional to the retention factor. The longer a component is retained by the column, the greater is the capacity factor.
Volume of Mobile Phase - (Measured in Cubic Meter) - The Volume of Mobile Phase is the amount of the solvent that runs through the chromatography column.
Partition Coefficient - The Partition Coefficient is defined as the ratio of the equilibrium concentrations of a dissolved substance in a two-phase system consisting of two largely immiscible solvents.

STEP 1: Convert Input(s) to Base Unit

Capacity Factor: 3 --> No Conversion Required
Volume of Mobile Phase: 5 Liter --> 0.005 Cubic Meter (Check conversion here)
Partition Coefficient: 40 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

V_s = (k^'*V_{mobile phase})/K --> (3*0.005)/40

Evaluating ... ...

V_s = 0.000375

STEP 3: Convert Result to Output's Unit

0.000375 Cubic Meter -->0.375 Liter (Check conversion here)

FINAL ANSWER

0.375 Liter <-- Volume of Stationary Phase

(Calculation completed in 00.004 seconds)

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Home » Chemistry » Analytical chemistry » Method of Separation Technique » Volume and Concentration of Mobile and Stationary Phase » Volume of Stationary Phase given Capacity Factor and Partition Coefficient

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< 6 Volume and Concentration of Mobile and Stationary Phase Calculators

Concentration of Mobile Phase given Capacity Factor

Go Concentration of Mobile Phase = (Volume of Stationary Phase*Concentration of Stationary Phase)/(Volume of Mobile Phase*Capacity Factor)

Volume of Mobile Phase given Capacity Factor

Go Volume of Mobile Phase = (Volume of Stationary Phase*Concentration of Stationary Phase)/(Capacity Factor*Concentration of Mobile Phase)

Concentration of Stationary Phase given Capacity Factor

Go Concentration of Stationary Phase = (Concentration of Mobile Phase*Volume of Mobile Phase*Capacity Factor)/Volume of Stationary Phase

Volume of Stationary Phase given Capacity Factor

Go Volume of Stationary Phase = (Volume of Mobile Phase*Concentration of Mobile Phase*Capacity Factor)/Concentration of Stationary Phase

Volume of Stationary Phase given Capacity Factor and Partition Coefficient

Go Volume of Stationary Phase = (Capacity Factor*Volume of Mobile Phase)/Partition Coefficient

Volume of Mobile Phase given Capacity Factor and Partition Coefficient

Go Volume of Mobile Phase = Partition Coefficient*(Volume of Stationary Phase/Capacity Factor)

Volume of Stationary Phase given Capacity Factor and Partition Coefficient Formula

Volume of Stationary Phase = (Capacity Factor*Volume of Mobile Phase)/Partition Coefficient
V_s = (k^'*V_{mobile phase})/K

What is Chromatography?

A separation process based on the various partitioning coefficients of different solutes between the two phases.
Involving the interaction of solute(s) and two phases
Mobile phase: A gas or liquid that moves through the column.
Stationary phase: A solid or liquid that remains in place.

What are the types of Chromatography?

1) Adsorption chromatography
2) Ion-exchange chromatography
3) Partition chromatography
4) Molecular Size exclusion chromatography
5) Affinity chromatography

How to Calculate Volume of Stationary Phase given Capacity Factor and Partition Coefficient?

Volume of Stationary Phase given Capacity Factor and Partition Coefficient calculator uses Volume of Stationary Phase = (Capacity Factor*Volume of Mobile Phase)/Partition Coefficient to calculate the Volume of Stationary Phase, The Volume of stationary phase given capacity factor and partition coefficient formula is defined as the ratio of the volume of mobile phase and capacity factor to the partition coefficient of the solutes. Volume of Stationary Phase is denoted by V_s symbol.

How to calculate Volume of Stationary Phase given Capacity Factor and Partition Coefficient using this online calculator? To use this online calculator for Volume of Stationary Phase given Capacity Factor and Partition Coefficient, enter Capacity Factor (k^'), Volume of Mobile Phase (V_{mobile phase}) & Partition Coefficient (K) and hit the calculate button. Here is how the Volume of Stationary Phase given Capacity Factor and Partition Coefficient calculation can be explained with given input values -> 375 = (3*0.005)/40.

FAQ

What is Volume of Stationary Phase given Capacity Factor and Partition Coefficient?

The Volume of stationary phase given capacity factor and partition coefficient formula is defined as the ratio of the volume of mobile phase and capacity factor to the partition coefficient of the solutes and is represented as V_s = (k^'*V_{mobile phase})/K or Volume of Stationary Phase = (Capacity Factor*Volume of Mobile Phase)/Partition Coefficient. The Capacity factor is directly proportional to the retention factor. The longer a component is retained by the column, the greater is the capacity factor, The Volume of Mobile Phase is the amount of the solvent that runs through the chromatography column & The Partition Coefficient is defined as the ratio of the equilibrium concentrations of a dissolved substance in a two-phase system consisting of two largely immiscible solvents.

How to calculate Volume of Stationary Phase given Capacity Factor and Partition Coefficient?

The Volume of stationary phase given capacity factor and partition coefficient formula is defined as the ratio of the volume of mobile phase and capacity factor to the partition coefficient of the solutes is calculated using Volume of Stationary Phase = (Capacity Factor*Volume of Mobile Phase)/Partition Coefficient. To calculate Volume of Stationary Phase given Capacity Factor and Partition Coefficient, you need Capacity Factor (k^'), Volume of Mobile Phase (V_{mobile phase}) & Partition Coefficient (K). With our tool, you need to enter the respective value for Capacity Factor, Volume of Mobile Phase & Partition Coefficient and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Volume of Stationary Phase?

In this formula, Volume of Stationary Phase uses Capacity Factor, Volume of Mobile Phase & Partition Coefficient. We can use 1 other way(s) to calculate the same, which is/are as follows -

Volume of Stationary Phase = (Volume of Mobile Phase*Concentration of Mobile Phase*Capacity Factor)/Concentration of Stationary Phase

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