Dilution Factor of Bacteria Calculator

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✖Colony Forming Unit per ml is a measure of viable bacterial or fungal cells. In direct microscopic counts (cell counting using haemocytometer) where all cells, dead and living, are counted.ⓘ Colony Forming Unit per ml [CFU]			+10% -10%
✖Volume of Culture Plate is the the amount of a substance occupying a particular volume of culture plate.ⓘ Volume of Culture Plate [v_c]			+10% -10%
✖No. of Colonies is a group of bacteria, fungi, and other microorganisms grown on a solid agar medium.ⓘ No. of Colonies [n_c]			+10% -10%

✖Dilution Factor is the factor by which the stock solution is diluted.ⓘ Dilution Factor of Bacteria [DF]

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Formula

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Dilution Factor of Bacteria

Formula

`"DF" = ("CFU"*"v"_{"c"})/"n"_{"c"}`

Example

`"15555.56"=("14Cells/L"*"10m³")/"9"`

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Dilution Factor of Bacteria Solution

STEP 0: Pre-Calculation Summary

Formula Used

Dilution Factor = (Colony Forming Unit per ml*Volume of Culture Plate)/No. of Colonies
DF = (CFU*v_c)/n_c
This formula uses 4 Variables

Variables Used

Dilution Factor - Dilution Factor is the factor by which the stock solution is diluted.
Colony Forming Unit per ml - (Measured in Cells per Cubic meter) - Colony Forming Unit per ml is a measure of viable bacterial or fungal cells. In direct microscopic counts (cell counting using haemocytometer) where all cells, dead and living, are counted.
Volume of Culture Plate - (Measured in Cubic Meter) - Volume of Culture Plate is the the amount of a substance occupying a particular volume of culture plate.
No. of Colonies - No. of Colonies is a group of bacteria, fungi, and other microorganisms grown on a solid agar medium.

STEP 1: Convert Input(s) to Base Unit

Colony Forming Unit per ml: 14 Cells per Liter --> 14000 Cells per Cubic meter (Check conversion here)
Volume of Culture Plate: 10 Cubic Meter --> 10 Cubic Meter No Conversion Required
No. of Colonies: 9 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

DF = (CFU*v_c)/n_c --> (14000*10)/9

Evaluating ... ...

DF = 15555.5555555556

STEP 3: Convert Result to Output's Unit

15555.5555555556 --> No Conversion Required

FINAL ANSWER

15555.5555555556 ≈ 15555.56 <-- Dilution Factor

(Calculation completed in 00.004 seconds)

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Created by Soupayan banerjee

National University of Judicial Science (NUJS), Kolkata

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< 8 Bacteriology Calculators

Growth Rate Constant of Bacteria

Go Growth Rate Constant = log2(No. of Bacteria at time t/Initial No. of Bacteria)/Time for bacteria

No. of Bacteria at Time T

Go No. of Bacteria at time t = (2^(Growth Rate Constant*Time for bacteria))*Initial No. of Bacteria

Volume of Culture Plate of Bacteria

Go Volume of Culture Plate = (No. of Colonies*Dilution Factor)/Colony Forming Unit per ml

Colony Forming Unit of Bacteria

Go Colony Forming Unit per ml = (No. of Colonies*Dilution Factor)/Volume of Culture Plate

Dilution Factor of Bacteria

Go Dilution Factor = (Colony Forming Unit per ml*Volume of Culture Plate)/No. of Colonies

No. of Colonies of Bacteria

Go No. of Colonies = (Colony Forming Unit per ml*Volume of Culture Plate)/Dilution Factor

No. of Generation using Generation Time for Bacteria

Go No. of Generation = Time for bacteria/Generation time

Growth Rate of Bacteria

Go Generation time = Time for bacteria/No. of Generation

Dilution Factor of Bacteria Formula

Dilution Factor = (Colony Forming Unit per ml*Volume of Culture Plate)/No. of Colonies
DF = (CFU*v_c)/n_c

What is CFU?

A colony forming unit, or CFU, is a unit commonly used to estimate the concentration of microorganisms in a test sample. The number of visible colonies (CFU) present on an agar plate can be multiplied by the dilution factor to provide a CFU/ml result.

How to Calculate Dilution Factor of Bacteria?

Dilution Factor of Bacteria calculator uses Dilution Factor = (Colony Forming Unit per ml*Volume of Culture Plate)/No. of Colonies to calculate the Dilution Factor, The Dilution Factor of Bacteria formula is defined as the factor by which the culture of bacteria is diluted. Dilution Factor is denoted by DF symbol.

How to calculate Dilution Factor of Bacteria using this online calculator? To use this online calculator for Dilution Factor of Bacteria, enter Colony Forming Unit per ml (CFU), Volume of Culture Plate (v_c) & No. of Colonies (n_c) and hit the calculate button. Here is how the Dilution Factor of Bacteria calculation can be explained with given input values -> 15555.56 = (14000*10)/9.

FAQ

What is Dilution Factor of Bacteria?

The Dilution Factor of Bacteria formula is defined as the factor by which the culture of bacteria is diluted and is represented as DF = (CFU*v_c)/n_c or Dilution Factor = (Colony Forming Unit per ml*Volume of Culture Plate)/No. of Colonies. Colony Forming Unit per ml is a measure of viable bacterial or fungal cells. In direct microscopic counts (cell counting using haemocytometer) where all cells, dead and living, are counted, Volume of Culture Plate is the the amount of a substance occupying a particular volume of culture plate & No. of Colonies is a group of bacteria, fungi, and other microorganisms grown on a solid agar medium.

How to calculate Dilution Factor of Bacteria?

The Dilution Factor of Bacteria formula is defined as the factor by which the culture of bacteria is diluted is calculated using Dilution Factor = (Colony Forming Unit per ml*Volume of Culture Plate)/No. of Colonies. To calculate Dilution Factor of Bacteria, you need Colony Forming Unit per ml (CFU), Volume of Culture Plate (v_c) & No. of Colonies (n_c). With our tool, you need to enter the respective value for Colony Forming Unit per ml, Volume of Culture Plate & No. of Colonies and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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