Dissociation Constant of Enzyme Substrate Complex given Modifying Factor of Enzyme Substrate Solution

STEP 0: Pre-Calculation Summary
Formula Used
Enzyme Substrate Dissociation Constant = Inhibitor Concentration/(Enzyme Substrate Modifying Factor-1)
Ki' = I/(α'-1)
This formula uses 3 Variables
Variables Used
Enzyme Substrate Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.
Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Enzyme Substrate Modifying Factor - The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.
STEP 1: Convert Input(s) to Base Unit
Inhibitor Concentration: 9 Mole per Liter --> 9000 Mole per Cubic Meter (Check conversion here)
Enzyme Substrate Modifying Factor: 2 --> No Conversion Required
STEP 2: Evaluate Formula
Substituting Input Values in Formula
Ki' = I/(α'-1) --> 9000/(2-1)
Evaluating ... ...
Ki' = 9000
STEP 3: Convert Result to Output's Unit
9000 Mole per Cubic Meter -->9 Mole per Liter (Check conversion here)
FINAL ANSWER
9 Mole per Liter <-- Enzyme Substrate Dissociation Constant
(Calculation completed in 00.004 seconds)

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Created by Prashant Singh
K J Somaiya College of science (K J Somaiya), Mumbai
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23 Competitive Inhibitor Calculators

Final Rate Constant for Competitive Inhibition of Enzyme Catalysis
Go Final Rate Constant for Catalysis = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Initial Enzyme Concentration*Substrate Concentration)
Inhibitor Concentration for Competitive Inhibition of Enzyme Catalysis
Go Inhibitor Concentration given IEC = (((((Final Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant
Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis
Go Initial Enzyme Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Final Rate Constant*Substrate Concentration)
Substrate Concentration of Competitive Inhibition of Enzyme Catalysis
Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Final Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)
Dissociation Constant for Competitive Inhibition of Enzyme Catalysis
Go Enzyme Inhibitor Dissociation Constant = Inhibitor Concentration/(((((Final Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/Michaelis Constant)-1)
Michaelis Constant for Competitive Inhibition of Enzyme Catalysis
Go Michaelis Constant = (((Final Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))
Initial Rate of System of Competitive Inhibition of Enzyme Catalysis
Go Initial Reaction Rate = (Final Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration)
Substrate Concentration given Modifying Factor in Michaelis Menten Equation
Go Substrate Concentration = (Initial Reaction Rate*(Enzyme Modifying Factor/Enzyme Substrate Modifying Factor)*Michaelis Constant)/(((1/Enzyme Substrate Modifying Factor)*Maximum Rate)-Initial Reaction Rate)
Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration
Go Substrate Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration)
Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration
Go Inhibitor Concentration = (((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant
Michaelis Constant in Competitive Inhibition given Enzyme Substrate Complex Concentration
Go Michaelis Constant = (((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))
Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration
Go Initial Enzyme Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Substrate Concentration)
Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis
Go Enzyme Substrate Complex Concentration = (Substrate Concentration*Initial Enzyme Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration)
Inhibitor Concentration in Competitive Inhibition given Maximum Rate of System
Go Inhibitor Concentration given Max Rate = (((((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant
Initial Rate in Competitive Inhibition given Maximum Rate of system
Go Initial Reaction Rate in CI = (Maximum Rate*Substrate Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration)
Dissociation Constant in Competitive Inhibition given Maximum Rate of System
Go Enzyme Inhibitor Dissociation Constant = Inhibitor Concentration/(((((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/Michaelis Constant)-1)
Michaelis Constant in Competitive Inhibition given Maximum Rate of System
Go Michaelis Constant = (((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))
Substrate Concentration in Competitive Inhibition given Maximum Rate of System
Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/(Maximum Rate-Initial Reaction Rate)
Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition
Go Apparent Michaelis Constant = (Substrate Concentration*(Maximum Rate-Initial Reaction Rate))/Initial Reaction Rate
Substrate Concentration given Apparent value of Michaelis Menten Constant
Go Substrate Concentration = (Apparent Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)
Dissociation Constant of Enzyme Substrate Complex given Modifying Factor of Enzyme Substrate
Go Enzyme Substrate Dissociation Constant = Inhibitor Concentration/(Enzyme Substrate Modifying Factor-1)
Dissociation Constant of Enzyme given Modifying Factor of Enzyme
Go Enzyme Inhibitor Dissociation Constant given MF = Inhibitor Concentration/(Enzyme Modifying Factor-1)
Modifying Factor of Enzyme
Go Enzyme Modifying Factor = 1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)

Dissociation Constant of Enzyme Substrate Complex given Modifying Factor of Enzyme Substrate Formula

Enzyme Substrate Dissociation Constant = Inhibitor Concentration/(Enzyme Substrate Modifying Factor-1)
Ki' = I/(α'-1)

What is competitive Inhibition?

In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time,this usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site.This type of inhibition can overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.

How to Calculate Dissociation Constant of Enzyme Substrate Complex given Modifying Factor of Enzyme Substrate?

Dissociation Constant of Enzyme Substrate Complex given Modifying Factor of Enzyme Substrate calculator uses Enzyme Substrate Dissociation Constant = Inhibitor Concentration/(Enzyme Substrate Modifying Factor-1) to calculate the Enzyme Substrate Dissociation Constant, The Dissociation constant of enzyme substrate complex given modifying factor of enzyme substrate formula is defined as the relation with inhibitor concentration and with the modifying factor of enzyme-substrate complex of the system. Enzyme Substrate Dissociation Constant is denoted by Ki' symbol.

How to calculate Dissociation Constant of Enzyme Substrate Complex given Modifying Factor of Enzyme Substrate using this online calculator? To use this online calculator for Dissociation Constant of Enzyme Substrate Complex given Modifying Factor of Enzyme Substrate, enter Inhibitor Concentration (I) & Enzyme Substrate Modifying Factor ') and hit the calculate button. Here is how the Dissociation Constant of Enzyme Substrate Complex given Modifying Factor of Enzyme Substrate calculation can be explained with given input values -> 0.009 = 9000/(2-1).

FAQ

What is Dissociation Constant of Enzyme Substrate Complex given Modifying Factor of Enzyme Substrate?
The Dissociation constant of enzyme substrate complex given modifying factor of enzyme substrate formula is defined as the relation with inhibitor concentration and with the modifying factor of enzyme-substrate complex of the system and is represented as Ki' = I/(α'-1) or Enzyme Substrate Dissociation Constant = Inhibitor Concentration/(Enzyme Substrate Modifying Factor-1). The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system & The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.
How to calculate Dissociation Constant of Enzyme Substrate Complex given Modifying Factor of Enzyme Substrate?
The Dissociation constant of enzyme substrate complex given modifying factor of enzyme substrate formula is defined as the relation with inhibitor concentration and with the modifying factor of enzyme-substrate complex of the system is calculated using Enzyme Substrate Dissociation Constant = Inhibitor Concentration/(Enzyme Substrate Modifying Factor-1). To calculate Dissociation Constant of Enzyme Substrate Complex given Modifying Factor of Enzyme Substrate, you need Inhibitor Concentration (I) & Enzyme Substrate Modifying Factor '). With our tool, you need to enter the respective value for Inhibitor Concentration & Enzyme Substrate Modifying Factor and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
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