Fluoroscence Intensity Calculator

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Physical spectroscopy

Gaseous state

Vapour Pressure

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Emission Spectroscopy

Absorption Spectroscopy

Raman spectroscopy

Rotational Spectroscopy

Vibrational Spectroscopy

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Fluoroscence and Phosphorescence

Quantum Yield and Singlet Llifetime

✖Rate Constant of Fluoroscence is the rate at which spontaneous emission occurs.ⓘ Rate Constant of Fluoroscence [K_f]			+10% -10%
✖Absorption Intensity obtained by integrating the area under the absorption line—is proportional to the amount of the absorbing substance present.ⓘ Absorption Intensity [I_a]			+10% -10%
✖Rate Constant of Non Radiative Reaction is defined as the rate at which deactivation occurs in the form of heat energy.ⓘ Rate Constant of Non Radiative Reaction [K_NR]			+10% -10%

✖Fluorosence Intensity formula is defined as the power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy.ⓘ Fluoroscence Intensity [I_F]

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Formula

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Fluoroscence Intensity

Formula

`"I"_{"F"} = ("K"_{"f"}*"I"_{"a"})/("K"_{"f"}+"K"_{"NR"})`

Example

`"238.8535W/m²"=("750rev/s"*"250W/m²")/("750rev/s"+"35rev/s")`

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Fluoroscence Intensity Solution

STEP 0: Pre-Calculation Summary

Formula Used

Fluorosence Intensity = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)
I_F = (K_f*I_a)/(K_f+K_NR)
This formula uses 4 Variables

Variables Used

Fluorosence Intensity - (Measured in Watt per Square Meter) - Fluorosence Intensity formula is defined as the power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy.
Rate Constant of Fluoroscence - (Measured in Hertz) - Rate Constant of Fluoroscence is the rate at which spontaneous emission occurs.
Absorption Intensity - (Measured in Watt per Square Meter) - Absorption Intensity obtained by integrating the area under the absorption line—is proportional to the amount of the absorbing substance present.
Rate Constant of Non Radiative Reaction - (Measured in Hertz) - Rate Constant of Non Radiative Reaction is defined as the rate at which deactivation occurs in the form of heat energy.

STEP 1: Convert Input(s) to Base Unit

Rate Constant of Fluoroscence: 750 Revolution per Second --> 750 Hertz (Check conversion here)
Absorption Intensity: 250 Watt per Square Meter --> 250 Watt per Square Meter No Conversion Required
Rate Constant of Non Radiative Reaction: 35 Revolution per Second --> 35 Hertz (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

I_F = (K_f*I_a)/(K_f+K_NR) --> (750*250)/(750+35)

Evaluating ... ...

I_F = 238.853503184713

STEP 3: Convert Result to Output's Unit

238.853503184713 Watt per Square Meter --> No Conversion Required

FINAL ANSWER

238.853503184713 ≈ 238.8535 Watt per Square Meter <-- Fluorosence Intensity

(Calculation completed in 00.004 seconds)

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< 25 Emission Spectroscopy Calculators

Intensity of Fluorescence given Degree of Exciplex Formation

Go Fluorosence Intensity given Degree of Exciplex = Rate Constant of Fluoroscence*Equilibrium Constant for Coordinate Complexes*(1-Degree of Exciplex Formation)/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)

Degree of Exciplex Formation

Go Degree of Exciplex Formation = (Equilibrium Constant for Coordinate Complexes*Quencher Concentration given Degree of Exciplex)/(1+(Equilibrium Constant for Coordinate Complexes*Quencher Concentration given Degree of Exciplex))

Fluoroscence Quantum Yield given Phosphorescence Quantum Yield

Go Fluorosecence Quantum Yield given Ph = Phosphosecence Quantum Yield*((Rate Constant of Fluoroscence*Singlet State Concentration)/(Phosphorescence Rate Constant*Concentration of Triplet State))

Fluorosence Intensity at Low Concentration of Solute

Go Fluorosence Intensity at Low Concentration = Fluorosecence Quantum Yield*Initial Intensity*2.303*Spectroscopical Molar Extinction Coefficient*Concentration at Time t*Length

Fluorescence Quantum Yield

Go Quantum Yield of Fluorescence = Rate of Radiative Reaction/(Rate of Radiative Reaction+Rate of Internal Conversion+Rate Constant of Intersystem Crossing+Quenching Constant)

Initial Intensity given Degree of Exciplex Formation

Go Initial Intensity given Degree of Exciplex = Rate Constant of Fluoroscence*Equilibrium Constant for Coordinate Complexes/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)

Intensity Ratio

Go Intensity Ratio = 1+(Quencher Concentration given Degree of Exciplex*(Quenching Constant/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)))

Quantum Yield of Fluorescence

Go Quantum Yield of Fluorescence = Rate Constant of Fluoroscence/(Rate Constant of Fluoroscence+Rate of Internal Conversion+Rate Constant of Intersystem Crossing)

Singlet Life Time of Radiative Process

Go Singlet Life time of Radiative Process = ((Initial Intensity/Fluorosence Intensity)-1)/(Quenching Constant*Quencher Concentration given Degree of Exciplex)

Fluoroscence Intensity without Quenching

Go Intensity Without Quenching = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Non Radiative Reaction+Rate Constant of Fluoroscence)

Final Intensity using Stern Volmer Equation

Go Final Intensity = Initial Intensity/(1+(Singlet Life time given Degree of Exciplex*Quenching Constant*Quencher Concentration given Degree of Exciplex))

Fluoroscence Intensity

Go Fluorosence Intensity = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)

Singlet Life Time

Go Singlet Life time = 1/(Rate Constant of Intersystem Crossing+Rate of Radiative Reaction+Rate of Internal Conversion+Quenching Constant)

Collisional Energy Transfer

Go Rate of Collisional Energy Transfer = Quenching Constant*Quencher Concentration given Degree of Exciplex*Singlet State Concentration

Rate of Deactivation

Go Rate of Deactivation = (Rate Constant of Non Radiative Reaction+Rate Constant of Fluoroscence)*Singlet State Concentration

Quenching Concentration given Degree of Exciplex Formation

Go Quencher Concentration given Degree of Exciplex = ((1/(1-Degree of Exciplex Formation))-1)*(1/Equilibrium Constant for Coordinate Complexes)

Quenching Concentration

Go Quencher Concentration = ((Initial Intensity/Fluorosence Intensity)-1)/Stern Volmner Constant

Singlet Life given Degree of Exciplex Formation

Go Singlet Life time given Degree of Exciplex = 1/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)

Rate of Phosphorescence

Go Phosphorescence Rate = Phosphorescence Rate Constant*Concentration of Triplet State

Fluorescence Rate Constant

Go Rate Constant of Fluoroscence = Rate of Fluoroscence/Singlet State Concentration

Rate of Activation

Go Rate of Activation = Equilibrium Constant*(1-Degree of Dissociation of Emission)

ISC Rate Constant

Go Rate Constant of ISC = Rate of Intersystem Crossing*Singlet State Concentration

Difference in Acidity between Ground and Excited State

Go Difference in pka = pKa of Excited State-pKa of Ground State

Equilibrium Constant for Exciplex Formation

Go Equilibrium Constant for Coordinate Complexes = 1/(1-Degree of Exciplex Formation)-1

Singlet Radiative Phosphorescence Lifetime

Go Singlet Radiative Phosphorescence Lifetime = 1/Rate of Phosphorescence

< 12 Fluoroscence and Phosphorescence Calculators

Intensity of Fluorescence given Degree of Exciplex Formation

Singlet State Concentration

Go Concentration of Singlet State = Absorption Intensity/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction+Rate Constant of Intersystem Crossing+Rate Constant of Internal Conversion)

Initial Intensity given Degree of Exciplex Formation

Go Initial Intensity given Degree of Exciplex = Rate Constant of Fluoroscence*Equilibrium Constant for Coordinate Complexes/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)

Intensity Ratio

Go Intensity Ratio = 1+(Quencher Concentration given Degree of Exciplex*(Quenching Constant/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)))

Fluoroscence Intensity without Quenching

Go Intensity Without Quenching = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Non Radiative Reaction+Rate Constant of Fluoroscence)

Fluoroscence Intensity

Go Fluorosence Intensity = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)

Quenching Concentration

Go Quencher Concentration = ((Initial Intensity/Fluorosence Intensity)-1)/Stern Volmner Constant

Phosphorescence Rate Constant

Go Rate Constant of Phosphorescence = Rate of Phosphorescence/Concentration of Triplet State

Rate of Phosphorescence

Go Phosphorescence Rate = Phosphorescence Rate Constant*Concentration of Triplet State

Fluorescence Rate Constant

Go Rate Constant of Fluoroscence = Rate of Fluoroscence/Singlet State Concentration

ISC Rate Constant

Go Rate Constant of ISC = Rate of Intersystem Crossing*Singlet State Concentration

Singlet Radiative Fluorescence Lifetime

Go Singlet Radiative Fluorescence Lifetime = 1/Rate Constant of Fluoroscence

Fluoroscence Intensity Formula

Fluorosence Intensity = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)
I_F = (K_f*I_a)/(K_f+K_NR)

What is the difference between absorbance and fluorescence intensity?

Absorbance is measured as the difference in intensity between light passing through the reference and the sample, whereas fluorescence is measured directly without any reference beam. In other words, fluorescence is measured over a dark background, while absorbance is measured over a bright background.

What affects fluorescence intensity?

Three important factors influencing the intensity of fluorescence emission were theoretical analyzed, including the absorption ability of excitation photons, fluorescence quantum yield, and fluorescence saturation & fluorescence quenching.

How to Calculate Fluoroscence Intensity?

Fluoroscence Intensity calculator uses Fluorosence Intensity = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction) to calculate the Fluorosence Intensity, The Fluoroscence Intensity indicates how much light (photons) is emitted. It is the extent of emission and it depends on the concentration of the excited fluorophore. Fluorescence is created by the absorption of energy (light) by fluorescent molecules, called fluorophores. Fluorosence Intensity is denoted by I_F symbol.

How to calculate Fluoroscence Intensity using this online calculator? To use this online calculator for Fluoroscence Intensity, enter Rate Constant of Fluoroscence (K_f), Absorption Intensity (I_a) & Rate Constant of Non Radiative Reaction (K_NR) and hit the calculate button. Here is how the Fluoroscence Intensity calculation can be explained with given input values -> 238.8535 = (750*250)/(750+35).

FAQ

What is Fluoroscence Intensity?

The Fluoroscence Intensity indicates how much light (photons) is emitted. It is the extent of emission and it depends on the concentration of the excited fluorophore. Fluorescence is created by the absorption of energy (light) by fluorescent molecules, called fluorophores and is represented as I_F = (K_f*I_a)/(K_f+K_NR) or Fluorosence Intensity = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction). Rate Constant of Fluoroscence is the rate at which spontaneous emission occurs, Absorption Intensity obtained by integrating the area under the absorption line—is proportional to the amount of the absorbing substance present & Rate Constant of Non Radiative Reaction is defined as the rate at which deactivation occurs in the form of heat energy.

How to calculate Fluoroscence Intensity?

The Fluoroscence Intensity indicates how much light (photons) is emitted. It is the extent of emission and it depends on the concentration of the excited fluorophore. Fluorescence is created by the absorption of energy (light) by fluorescent molecules, called fluorophores is calculated using Fluorosence Intensity = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction). To calculate Fluoroscence Intensity, you need Rate Constant of Fluoroscence (K_f), Absorption Intensity (I_a) & Rate Constant of Non Radiative Reaction (K_NR). With our tool, you need to enter the respective value for Rate Constant of Fluoroscence, Absorption Intensity & Rate Constant of Non Radiative Reaction and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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