Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration Calculator

✖The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.ⓘ Initial Enzyme Concentration [[E₀]]			+10% -10%
✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration [S]			+10% -10%
✖The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.ⓘ Enzyme Substrate Complex Concentration [ES]			+10% -10%
✖The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.ⓘ Michaelis Constant [K_M]			+10% -10%
✖The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.ⓘ Enzyme Inhibitor Dissociation Constant [K_i]			+10% -10%

✖The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.ⓘ Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration [I]

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Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration

Formula

`"I" = (((((("[E"_{"0"}"]")*"S")/"ES")-"S")/"K"_{"M"})-1)*"K"_{"i"}`

Example

`"66.5mol/L"=((((("100mol/L"*"1.5mol/L")/"10mol/L")-"1.5mol/L")/"3mol/L")-1)*"19mol/L"`

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Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration Solution

STEP 0: Pre-Calculation Summary

Formula Used

Inhibitor Concentration = (((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant
I = ((((([E₀]*S)/ES)-S)/K_M)-1)*K_i
This formula uses 6 Variables

Variables Used

Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Enzyme Inhibitor Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.

STEP 1: Convert Input(s) to Base Unit

Initial Enzyme Concentration: 100 Mole per Liter --> 100000 Mole per Cubic Meter (Check conversion here)
Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion here)
Enzyme Substrate Complex Concentration: 10 Mole per Liter --> 10000 Mole per Cubic Meter (Check conversion here)
Michaelis Constant: 3 Mole per Liter --> 3000 Mole per Cubic Meter (Check conversion here)
Enzyme Inhibitor Dissociation Constant: 19 Mole per Liter --> 19000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

I = ((((([E₀]*S)/ES)-S)/K_M)-1)*K_i --> (((((100000*1500)/10000)-1500)/3000)-1)*19000

Evaluating ... ...

I = 66500

STEP 3: Convert Result to Output's Unit

66500 Mole per Cubic Meter -->66.5 Mole per Liter (Check conversion here)

FINAL ANSWER

66.5 Mole per Liter <-- Inhibitor Concentration

(Calculation completed in 00.020 seconds)

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< 23 Competitive Inhibitor Calculators

Final Rate Constant for Competitive Inhibition of Enzyme Catalysis

Go Final Rate Constant for Catalysis = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Initial Enzyme Concentration*Substrate Concentration)

Inhibitor Concentration for Competitive Inhibition of Enzyme Catalysis

Go Inhibitor Concentration given IEC = (((((Final Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant

Initial Enzyme Concentration of Competitive Inhibition of Enzyme Catalysis

Go Initial Enzyme Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Final Rate Constant*Substrate Concentration)

Substrate Concentration of Competitive Inhibition of Enzyme Catalysis

Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Final Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Dissociation Constant for Competitive Inhibition of Enzyme Catalysis

Go Enzyme Inhibitor Dissociation Constant = Inhibitor Concentration/(((((Final Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/Michaelis Constant)-1)

Michaelis Constant for Competitive Inhibition of Enzyme Catalysis

Go Michaelis Constant = (((Final Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))

Initial Rate of System of Competitive Inhibition of Enzyme Catalysis

Go Initial Reaction Rate = (Final Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration)

Substrate Concentration given Modifying Factor in Michaelis Menten Equation

Go Substrate Concentration = (Initial Reaction Rate*(Enzyme Modifying Factor/Enzyme Substrate Modifying Factor)*Michaelis Constant)/(((1/Enzyme Substrate Modifying Factor)*Maximum Rate)-Initial Reaction Rate)

Substrate Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration

Go Substrate Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/((Initial Enzyme Concentration)-Enzyme Substrate Complex Concentration)

Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration

Go Inhibitor Concentration = (((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant

Michaelis Constant in Competitive Inhibition given Enzyme Substrate Complex Concentration

Go Michaelis Constant = (((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))

Initial Enzyme in Competitive Inhibition given Enzyme Substrate Complex Concentration

Go Initial Enzyme Concentration = (Enzyme Substrate Complex Concentration*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Substrate Concentration)

Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis

Go Enzyme Substrate Complex Concentration = (Substrate Concentration*Initial Enzyme Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration)

Inhibitor Concentration in Competitive Inhibition given Maximum Rate of System

Go Inhibitor Concentration given Max Rate = (((((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant

Initial Rate in Competitive Inhibition given Maximum Rate of system

Go Initial Reaction Rate in CI = (Maximum Rate*Substrate Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration)

Dissociation Constant in Competitive Inhibition given Maximum Rate of System

Go Enzyme Inhibitor Dissociation Constant = Inhibitor Concentration/(((((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/Michaelis Constant)-1)

Michaelis Constant in Competitive Inhibition given Maximum Rate of System

Go Michaelis Constant = (((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))

Substrate Concentration in Competitive Inhibition given Maximum Rate of System

Go Substrate Concentration = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))))/(Maximum Rate-Initial Reaction Rate)

Apparent Value of Michaelis Menten Constant in Presence of Competitive Inhibition

Go Apparent Michaelis Constant = (Substrate Concentration*(Maximum Rate-Initial Reaction Rate))/Initial Reaction Rate

Substrate Concentration given Apparent value of Michaelis Menten Constant

Go Substrate Concentration = (Apparent Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)

Dissociation Constant of Enzyme Substrate Complex given Modifying Factor of Enzyme Substrate

Go Enzyme Substrate Dissociation Constant = Inhibitor Concentration/(Enzyme Substrate Modifying Factor-1)

Dissociation Constant of Enzyme given Modifying Factor of Enzyme

Go Enzyme Inhibitor Dissociation Constant given MF = Inhibitor Concentration/(Enzyme Modifying Factor-1)

Modifying Factor of Enzyme

Go Enzyme Modifying Factor = 1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)

Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration Formula

What is competitive Inhibition?

In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time,this usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site.This type of inhibition can overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.

How to Calculate Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration?

Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration calculator uses Inhibitor Concentration = (((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant to calculate the Inhibitor Concentration, The Inhibitor concentration in competitive inhibition given enzyme substrate complex concentration formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km. Inhibitor Concentration is denoted by I symbol.

How to calculate Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration using this online calculator? To use this online calculator for Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration, enter Initial Enzyme Concentration ([E₀]), Substrate Concentration (S), Enzyme Substrate Complex Concentration (ES), Michaelis Constant (K_M) & Enzyme Inhibitor Dissociation Constant (K_i) and hit the calculate button. Here is how the Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration calculation can be explained with given input values -> 0.0665 = (((((100000*1500)/10000)-1500)/3000)-1)*19000.

FAQ

What is Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration?

The Inhibitor concentration in competitive inhibition given enzyme substrate complex concentration formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km and is represented as I = ((((([E₀]*S)/ES)-S)/K_M)-1)*K_i or Inhibitor Concentration = (((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant. The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction, The Substrate Concentration is the number of moles of substrate per liter solution, The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate, The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system & The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.

How to calculate Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration?

The Inhibitor concentration in competitive inhibition given enzyme substrate complex concentration formula is defined as a plot of the reaction velocity (V0) associated with the concentration [S] of the substrate can then be used to determine values such as Vmax, initial velocity, and Km is calculated using Inhibitor Concentration = (((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant. To calculate Inhibitor Concentration in Competitive Inhibition given Enzyme Substrate Complex Concentration, you need Initial Enzyme Concentration ([E₀]), Substrate Concentration (S), Enzyme Substrate Complex Concentration (ES), Michaelis Constant (K_M) & Enzyme Inhibitor Dissociation Constant (K_i). With our tool, you need to enter the respective value for Initial Enzyme Concentration, Substrate Concentration, Enzyme Substrate Complex Concentration, Michaelis Constant & Enzyme Inhibitor Dissociation Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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