How to Calculate Initial Reaction Rate at Low Substrate Concentration?
Initial Reaction Rate at Low Substrate Concentration calculator uses Initial Reaction Rate = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Michaelis Constant to calculate the Initial Reaction Rate, The Initial reaction rate at low substrate concentration formula is defined as the relation with initial enzyme concentration and the concentration of a substrate. Here substrate concentration is very less i.e negligible in comparison to Michaelis constant. Initial Reaction Rate is denoted by V_{0} symbol.
How to calculate Initial Reaction Rate at Low Substrate Concentration using this online calculator? To use this online calculator for Initial Reaction Rate at Low Substrate Concentration, enter Catalytic Rate Constant (k_{cat}), Initial Enzyme Concentration ([E_{0}]), Substrate Concentration (S) & Michaelis Constant (K_{M}) and hit the calculate button. Here is how the Initial Reaction Rate at Low Substrate Concentration calculation can be explained with given input values -> 0.0325 = (0.65*100000*1500)/3000.