Initial Reaction Rate in presence of Uncompetitive Inhibitor Solution

STEP 0: Pre-Calculation Summary
Formula Used
Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration))
V0 = (Vmax*S)/(KM+(α'*S))
This formula uses 5 Variables
Variables Used
Initial Reaction Rate - (Measured in Mole per Cubic Meter Second) - The Initial Reaction Rate is defined as the initial speed at which a chemical reaction takes place.
Maximum Rate - (Measured in Mole per Cubic Meter Second) - The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Michaelis Constant - (Measured in Mole per Cubic Meter) - The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system.
Enzyme Substrate Modifying Factor - The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.
STEP 1: Convert Input(s) to Base Unit
Maximum Rate: 40 Mole per Liter Second --> 40000 Mole per Cubic Meter Second (Check conversion here)
Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion here)
Michaelis Constant: 3 Mole per Liter --> 3000 Mole per Cubic Meter (Check conversion here)
Enzyme Substrate Modifying Factor: 2 --> No Conversion Required
STEP 2: Evaluate Formula
Substituting Input Values in Formula
V0 = (Vmax*S)/(KM+(α'*S)) --> (40000*1500)/(3000+(2*1500))
Evaluating ... ...
V0 = 10000
STEP 3: Convert Result to Output's Unit
10000 Mole per Cubic Meter Second -->10 Mole per Liter Second (Check conversion here)
FINAL ANSWER
10 Mole per Liter Second <-- Initial Reaction Rate
(Calculation completed in 00.004 seconds)

Credits

Created by Prashant Singh
K J Somaiya College of science (K J Somaiya), Mumbai
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12 Uncompetitive Inhibitor Calculators

Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor
Go Enzyme Substrate Modifying Factor = ((Maximum Rate*Substrate Concentration)-(Initial Reaction Rate*Michaelis Constant))/(Initial Reaction Rate*Substrate Concentration)
Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant
Go Enzyme Substrate Complex Concentration = (Forward Rate Constant*Catalyst Concentration*Substrate Concentration)/(Reverse Rate Constant+Catalytic Rate Constant)
Initial Reaction Rate in presence of Uncompetitive Inhibitor
Go Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration))
Maximum Reaction Rate in Presence of Uncompetitive Inhibitor
Go Maximum Rate = (Initial Reaction Rate*(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration)))/Substrate Concentration
Substrate Concentration in presence of Uncompetitive Inhibitor
Go Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor))
Michaelis Constant in Presence of Uncompetitive Inhibitor
Go Michaelis Constant = (Substrate Concentration*(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor)))/Initial Reaction Rate
Enzyme Substrate Inhibitor Concentration in presence of Uncompetitive Inhibitor
Go Enzyme Substrate Inhibitor Complex Concentration = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Dissociation Constant
Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor
Go Enzyme Substrate Dissociation Constant = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Inhibitor Complex Concentration
Enzyme Substrate complex Concentration in Presence of Uncompetitive Inhibitor
Go Enzyme Substrate Complex Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Inhibitor Concentration
Inhibitor Concentration in Presence of Uncompetitive Inhibitor
Go Inhibitor Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Enzyme Substrate Complex Concentration
Enzyme Substrate Modifying Factor given Enzyme Substrate Dissociation Constant
Go Enzyme Substrate Modifying Factor = (1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant))
Enzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor
Go Enzyme Substrate Dissociation Constant = Inhibitor Concentration/(Enzyme Substrate Modifying Factor-1)

Initial Reaction Rate in presence of Uncompetitive Inhibitor Formula

Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration))
V0 = (Vmax*S)/(KM+(α'*S))

What is Uncompetitive inhibitor?

Uncompetitive inhibition, also known as anti-competitive inhibition, takes place when an enzyme inhibitor binds only to the complex formed between the enzyme and the substrate (the E-S complex). Uncompetitive inhibition typically occurs in reactions with two or more substrates or products. Uncompetitive inhibition is distinguished from competitive inhibition by two observations: first uncompetitive inhibition cannot be reversed by increasing [S] and second, as shown, the Lineweaver–Burk plot yields parallel rather than intersecting lines.

How to Calculate Initial Reaction Rate in presence of Uncompetitive Inhibitor?

Initial Reaction Rate in presence of Uncompetitive Inhibitor calculator uses Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration)) to calculate the Initial Reaction Rate, The Initial reaction rate in presence of uncompetitive inhibitor formula is defined by the altered Michaelis-Menten equation. Initial Reaction Rate is denoted by V0 symbol.

How to calculate Initial Reaction Rate in presence of Uncompetitive Inhibitor using this online calculator? To use this online calculator for Initial Reaction Rate in presence of Uncompetitive Inhibitor, enter Maximum Rate (Vmax), Substrate Concentration (S), Michaelis Constant (KM) & Enzyme Substrate Modifying Factor ') and hit the calculate button. Here is how the Initial Reaction Rate in presence of Uncompetitive Inhibitor calculation can be explained with given input values -> 0.01 = (40000*1500)/(3000+(2*1500)).

FAQ

What is Initial Reaction Rate in presence of Uncompetitive Inhibitor?
The Initial reaction rate in presence of uncompetitive inhibitor formula is defined by the altered Michaelis-Menten equation and is represented as V0 = (Vmax*S)/(KM+(α'*S)) or Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration)). The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration, The Substrate Concentration is the number of moles of substrate per liter solution, The Michaelis Constant is numerically equal to the substrate concentration at which the reaction rate is half of the maximum rate of the system & The Enzyme Substrate Modifying Factor is defined by the inhibitor concentration and the dissociation constant of the enzyme-substrate complex.
How to calculate Initial Reaction Rate in presence of Uncompetitive Inhibitor?
The Initial reaction rate in presence of uncompetitive inhibitor formula is defined by the altered Michaelis-Menten equation is calculated using Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration)). To calculate Initial Reaction Rate in presence of Uncompetitive Inhibitor, you need Maximum Rate (Vmax), Substrate Concentration (S), Michaelis Constant (KM) & Enzyme Substrate Modifying Factor '). With our tool, you need to enter the respective value for Maximum Rate, Substrate Concentration, Michaelis Constant & Enzyme Substrate Modifying Factor and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
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