Mobile Phase Travel Time through Column Calculator

✖The Retention Time of solute is defined as the time taken by the solute to move over the stationary phase and to come out of the column.ⓘ Retention Time [t_r]			+10% -10%
✖The Adjusted Retention Time is the removal of time taken by the mobile phase to travel the column from the retention time.ⓘ Adjusted Retention Time [tr^']			+10% -10%

✖Unretained Solute Travel Time through Column s the time taken by the mobile phase to move over the length of the column.ⓘ Mobile Phase Travel Time through Column [t_C]

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Formula

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Mobile Phase Travel Time through Column

Formula

`"t"_{"C"} = ("t"_{"r"}-"tr"^{"'"})`

Example

`"11s"=("13s"-"2s")`

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Mobile Phase Travel Time through Column Solution

STEP 0: Pre-Calculation Summary

Formula Used

Unretained Solute Travel Time through Column = (Retention Time-Adjusted Retention Time)
t_C = (t_r-tr^')
This formula uses 3 Variables

Variables Used

Unretained Solute Travel Time through Column - (Measured in Second) - Unretained Solute Travel Time through Column s the time taken by the mobile phase to move over the length of the column.
Retention Time - (Measured in Second) - The Retention Time of solute is defined as the time taken by the solute to move over the stationary phase and to come out of the column.
Adjusted Retention Time - (Measured in Second) - The Adjusted Retention Time is the removal of time taken by the mobile phase to travel the column from the retention time.

STEP 1: Convert Input(s) to Base Unit

Retention Time: 13 Second --> 13 Second No Conversion Required
Adjusted Retention Time: 2 Second --> 2 Second No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

t_C = (t_r-tr^') --> (13-2)

Evaluating ... ...

t_C = 11

STEP 3: Convert Result to Output's Unit

11 Second --> No Conversion Required

FINAL ANSWER

11 Second <-- Unretained Solute Travel Time through Column

(Calculation completed in 00.004 seconds)

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Credits

Created by Prashant Singh

K J Somaiya College of science (K J Somaiya), Mumbai

Prashant Singh has created this Calculator and 700+ more calculators!

Verified by Prerana Bakli

University of Hawaiʻi at Mānoa (UH Manoa), Hawaii, USA

Prerana Bakli has verified this Calculator and 1600+ more calculators!

< 6 Phase Calculators

Molar Concentration of Third Component in Second Phase

Go Concentration of Solute in Phase2 = (Concentration of Solute in Solvent 1/Distribution Coefficient of Solution)

Molar Concentration of Third Component in First Phase

Go Concentration of Solute in Phase1 = (Distribution Coefficient of Solution*Solute Concentration in Solvent2)

Mobile Phase Travel Time through Column

Go Unretained Solute Travel Time through Column = (Retention Time-Adjusted Retention Time)

Total Concentration of Solute in Organic Phase

Go Concentration in Organic Solvent = (Distribution Ratio*Concentration in Aqueous Phase)

Total Concentration of Solute in Aqueous Phase

Go Concentration in Aqueous Solvent = (Concentration in Organic Phase/Distribution Ratio)

Mobile Phase Travel Time given Capacity Factor

Go Unretained Solute Travel Time given CP = (Retention Time)/(Capacity Factor+1)

< 13 Relative and Adjusted Retention and Phase Calculators

Molar Concentration of Third Component in Second Phase

Go Concentration of Solute in Phase2 = (Concentration of Solute in Solvent 1/Distribution Coefficient of Solution)

Molar Concentration of Third Component in First Phase

Go Concentration of Solute in Phase1 = (Distribution Coefficient of Solution*Solute Concentration in Solvent2)

Relative Retention given Adjusted Retention Times

Go Actual Relative Retention = (Adjusted Retention Time of Solute 2/Adjusted Retention Time of Solute 1)

Relative Retention given Partition Coefficient of Two Components

Go Actual Relative Retention = (Partition Coefficient of Solute 2/Partition Coefficient of Solute 1)

Adjusted Retention of Second Component given Relative Retention

Go Adjusted Retention Time of Comp 2 = (Relative Retention*Adjusted Retention Time of Solute 1)

Adjusted Retention of First Component given Relative Retention

Go Adjusted Retention Time of Comp 1 = (Adjusted Retention Time of Solute 2/Relative Retention)

Partition Coefficient of Solute 1 given Relative Retention

Go Partition Coefficient of Comp 1 = (Partition Coefficient of Solute 2/Relative Retention)

Partition Coefficient of Solute 2 given Relative Retention

Go Partition Coefficient of Comp 2 = (Relative Retention*Partition Coefficient of Solute 1)

Mobile Phase Travel Time through Column

Go Unretained Solute Travel Time through Column = (Retention Time-Adjusted Retention Time)

Total Concentration of Solute in Aqueous Phase

Go Concentration in Aqueous Solvent = (Concentration in Organic Phase/Distribution Ratio)

Total Concentration of Solute in Organic Phase

Go Concentration in Organic Solvent = (Distribution Ratio*Concentration in Aqueous Phase)

Relative Retention given Capacity Factor of Two Components

Go Actual Relative Retention = (Capacity Factor of Solute 2/Capacity Factor of Solute 1)

Mobile Phase Travel Time given Capacity Factor

Go Unretained Solute Travel Time given CP = (Retention Time)/(Capacity Factor+1)

Mobile Phase Travel Time through Column Formula

Unretained Solute Travel Time through Column = (Retention Time-Adjusted Retention Time)
t_C = (t_r-tr^')

What is Chromatography?

A separation process based on the various partitioning coefficients of different solutes between the two phases.
Involving the interaction of solute(s) and two phases
Mobile phase: A gas or liquid that moves through the column.
Stationary phase: A solid or liquid that remains in place.

What are the types of Chromatography?

1) Adsorption chromatography
2) Ion-exchange chromatography
3) Partition chromatography
4) Molecular Size exclusion chromatography
5) Affinity chromatography

How to Calculate Mobile Phase Travel Time through Column?

Mobile Phase Travel Time through Column calculator uses Unretained Solute Travel Time through Column = (Retention Time-Adjusted Retention Time) to calculate the Unretained Solute Travel Time through Column, The Mobile phase travel time through column formula is defined as subtraction of adjusted retention time from the retention time of the solute. Unretained Solute Travel Time through Column is denoted by t_C symbol.

How to calculate Mobile Phase Travel Time through Column using this online calculator? To use this online calculator for Mobile Phase Travel Time through Column, enter Retention Time (t_r) & Adjusted Retention Time (tr^') and hit the calculate button. Here is how the Mobile Phase Travel Time through Column calculation can be explained with given input values -> 11 = (13-2).

FAQ

What is Mobile Phase Travel Time through Column?

The Mobile phase travel time through column formula is defined as subtraction of adjusted retention time from the retention time of the solute and is represented as t_C = (t_r-tr^') or Unretained Solute Travel Time through Column = (Retention Time-Adjusted Retention Time). The Retention Time of solute is defined as the time taken by the solute to move over the stationary phase and to come out of the column & The Adjusted Retention Time is the removal of time taken by the mobile phase to travel the column from the retention time.

How to calculate Mobile Phase Travel Time through Column?

The Mobile phase travel time through column formula is defined as subtraction of adjusted retention time from the retention time of the solute is calculated using Unretained Solute Travel Time through Column = (Retention Time-Adjusted Retention Time). To calculate Mobile Phase Travel Time through Column, you need Retention Time (t_r) & Adjusted Retention Time (tr^'). With our tool, you need to enter the respective value for Retention Time & Adjusted Retention Time and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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