Retention Volume using Retention Factor Solution

STEP 0: Pre-Calculation Summary
Formula Used
Retention Volume = Void Volume*(Retention Factor+1)
VR = V0*(Rf+1)
This formula uses 3 Variables
Variables Used
Retention Volume - (Measured in Cubic Meter) - The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column.
Void Volume - (Measured in Cubic Meter) - Void Volume is the volume of mobile phase in a column.
Retention Factor - Retention Factor is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front.
STEP 1: Convert Input(s) to Base Unit
Void Volume: 5.6 Milliliter --> 5.6E-06 Cubic Meter (Check conversion here)
Retention Factor: 10 --> No Conversion Required
STEP 2: Evaluate Formula
Substituting Input Values in Formula
VR = V0*(Rf+1) --> 5.6E-06*(10+1)
Evaluating ... ...
VR = 6.16E-05
STEP 3: Convert Result to Output's Unit
6.16E-05 Cubic Meter -->0.0616 Liter (Check conversion here)
FINAL ANSWER
0.0616 Liter <-- Retention Volume
(Calculation completed in 00.004 seconds)

Credits

Created by Soupayan banerjee
National University of Judicial Science (NUJS), Kolkata
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Amity Institute Of Applied Sciences (AIAS, Amity University), Noida, India
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17 Osmolality Calculators

Peak Resolution in Chromatography
Go Peak Resolution = (Retention Volume of Molecule 2-Retention Volume of Molecule 1)/((Width of Chromatographic Peak of Molecule 1+Widths of Chromatographic Peak of Molecule 2)/2)
Blood Urea Nitrogen using Calculated Serum Osmolality
Go Blood Urea Nitrogen = 2.8*((Calculated Serum Osmolality)-(2*Serum Sodium)-(Serum Glucose/18))
Serum Glucose using Calculated Serum Osmolality
Go Serum Glucose = 18*((Calculated Serum Osmolality)-(2*Serum Sodium)-(Blood Urea Nitrogen/2.8))
Serum Sodium using Calculated Serum Osmolality
Go Serum Sodium = ((Calculated Serum Osmolality)-(Serum Glucose/18)-(Blood Urea Nitrogen/2.8))/2
Calculated Serum Osmolality
Go Calculated Serum Osmolality = (2*Serum Sodium)+(Serum Glucose/18)+(Blood Urea Nitrogen/2.8)
Width of Chromatographic Peak using Column Efficiency
Go Width of Chromatographic Peak = Retention Volume/(sqrt(Column Efficiency/16))
Retention Volume using Column Efficiency
Go Retention Volume = Width of Chromatographic Peak*(sqrt(Column Efficiency/16))
Column Efficiency in Chromatography
Go Column Efficiency = 16*((Retention Volume/ Width of Chromatographic Peak)^2)
Increase of Splicing Potential by Wild-type Sequence
Go Splicing Potential = log10(Weight Factor for Increase of Splicing Potential)
Calculated Serum Osmolality using Osmolar Gap
Go Calculated Serum Osmolality = Measured Osmolality-Osmolar Gap
Measured Osmolality using Osmolar Gap
Go Measured Osmolality = Osmolar Gap+Calculated Serum Osmolality
Osmolar Gap
Go Osmolar Gap = Measured Osmolality-Calculated Serum Osmolality
Total Volume of Mobile Phase within Column given Retention Factor
Go Void Volume = Retention Volume/(Retention Factor+1)
Retention Volume using Retention Factor
Go Retention Volume = Void Volume*(Retention Factor+1)
Decrease of Splicing Potential by Mutant Sequence
Go Splicing Potential = -log10(Weight Factor)
Plasma Osmolality
Go Plasma Osmolality = (2*Sodium Concentration in Plasma)
Plasma Concentration using Plasma Osmolality
Go Sodium Concentration in Plasma = Plasma Osmolality/2

Retention Volume using Retention Factor Formula

Retention Volume = Void Volume*(Retention Factor+1)
VR = V0*(Rf+1)

What is Column Chromatography?

Column chromatography separates substances based on differential adsorption of compounds to the adsorbent as the compounds move through the column at different rates which allow them to get separated in fractions. This technique can be used on a small scale as well as large scale to purify materials that can be used in future experiments. This method is a type of adsorption chromatography technique.

How to Calculate Retention Volume using Retention Factor?

Retention Volume using Retention Factor calculator uses Retention Volume = Void Volume*(Retention Factor+1) to calculate the Retention Volume, The Retention Volume using Retention Factor formula is defined as the volume that has passed through the column since the target molecule was introduced onto the column. Retention Volume is denoted by VR symbol.

How to calculate Retention Volume using Retention Factor using this online calculator? To use this online calculator for Retention Volume using Retention Factor, enter Void Volume (V0) & Retention Factor (Rf) and hit the calculate button. Here is how the Retention Volume using Retention Factor calculation can be explained with given input values -> 61.6 = 5.6E-06*(10+1).

FAQ

What is Retention Volume using Retention Factor?
The Retention Volume using Retention Factor formula is defined as the volume that has passed through the column since the target molecule was introduced onto the column and is represented as VR = V0*(Rf+1) or Retention Volume = Void Volume*(Retention Factor+1). Void Volume is the volume of mobile phase in a column & Retention Factor is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front.
How to calculate Retention Volume using Retention Factor?
The Retention Volume using Retention Factor formula is defined as the volume that has passed through the column since the target molecule was introduced onto the column is calculated using Retention Volume = Void Volume*(Retention Factor+1). To calculate Retention Volume using Retention Factor, you need Void Volume (V0) & Retention Factor (Rf). With our tool, you need to enter the respective value for Void Volume & Retention Factor and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
How many ways are there to calculate Retention Volume?
In this formula, Retention Volume uses Void Volume & Retention Factor. We can use 1 other way(s) to calculate the same, which is/are as follows -
  • Retention Volume = Width of Chromatographic Peak*(sqrt(Column Efficiency/16))
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