Slope of Absorbance vs Concentration Plot Calculator

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✖The Molar Extinction Coefficient is a measure of how strongly a chemical species or substance absorbs light at a particular wavelength.ⓘ Molar Extinction Coefficient [ε]			+10% -10%
✖Thickness of Cell is useful in calculating the concentration of a solution on the basis of its light absorption.ⓘ Thickness of Cell [l]			+10% -10%

✖The Slope of Line is a number that measures its "steepness", usually denoted by the letter m. It is the change in y for a unit change in x along the line.ⓘ Slope of Absorbance vs Concentration Plot [m]

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Formula

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Slope of Absorbance vs Concentration Plot

Formula

`"m" = "ε"*"l"`

Example

`"9.6E^-11"="19cm²/mol"*"50.5nm"`

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Slope of Absorbance vs Concentration Plot Solution

STEP 0: Pre-Calculation Summary

Formula Used

Slope of Line = Molar Extinction Coefficient*Thickness of Cell
m = ε*l
This formula uses 3 Variables

Variables Used

Slope of Line - The Slope of Line is a number that measures its "steepness", usually denoted by the letter m. It is the change in y for a unit change in x along the line.
Molar Extinction Coefficient - (Measured in Square Meter per Mole) - The Molar Extinction Coefficient is a measure of how strongly a chemical species or substance absorbs light at a particular wavelength.
Thickness of Cell - (Measured in Meter) - Thickness of Cell is useful in calculating the concentration of a solution on the basis of its light absorption.

STEP 1: Convert Input(s) to Base Unit

Molar Extinction Coefficient: 19 Square Centimeter per Mole --> 0.0019 Square Meter per Mole (Check conversion here)
Thickness of Cell: 50.5 Nanometer --> 5.05E-08 Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

m = ε*l --> 0.0019*5.05E-08

Evaluating ... ...

m = 9.595E-11

STEP 3: Convert Result to Output's Unit

9.595E-11 --> No Conversion Required

FINAL ANSWER

9.595E-11 ≈ 9.6E-11 <-- Slope of Line

(Calculation completed in 00.004 seconds)

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Created by Akshada Kulkarni

National Institute of Information Technology (NIIT), Neemrana

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K J Somaiya College of science (K J Somaiya), Mumbai

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< 15 Beer-Lambert law Calculators

Molar Extinction Coefficient given Intensities of Radiation

Go Molar Extinction Coefficient = log10(Intensity of Incident Radiation/Intensity of Transmitted Radiation)*(1/(Thickness of Cell*Concentration of Solution))

Thickness of Cell given Intensities of Radiation

Go Thickness of Cell = log10(Intensity of Incident Radiation/Intensity of Transmitted Radiation)*(1/(Molar Extinction Coefficient*Concentration of Solution))

Concentration of Solution given Intensities of Radiation

Go Concentration of Solution = log10(Intensity of Incident Radiation/Intensity of Reflected Radiation)*(1/(Thickness of Cell*Molar Extinction Coefficient))

Intensity of Transmitted Radiation given Concentration of Solution

Go Intensity of Transmitted Radiation = Intensity of Incident Radiation/exp(Molar Extinction Coefficient*Thickness of Cell*Concentration of Solution)

Intensity of Incident Radiation given Concentration of Solution

Go Intensity of Incident Radiation = Intensity of Transmitted Radiation*exp(Molar Extinction Coefficient*Concentration of Solution*Thickness of Cell)

Molar Extinction Coefficient

Go Molar Extinction Coefficient = Absorbance/(Concentration of Solution*Thickness of Cell)

Concentration of Solution

Go Concentration of Solution = Absorbance/(Thickness of Cell*Molar Extinction Coefficient)

Thickness of Cell

Go Thickness of Cell = Absorbance/(Molar Extinction Coefficient*Concentration of Solution)

Beer-Lambert law given Intensity of Radiation

Go Absorbance = log10(Intensity of Incident Radiation/Intensity of Transmitted Radiation)

Absorbance using Beer-Lambert Law

Go Absorbance = Molar Extinction Coefficient*Concentration of Solution*Thickness of Cell

Intensity of Transmitted Radiation

Go Intensity of Transmitted Radiation = Intensity of Incident Radiation/10^(Absorbance)

Intensity of Incident Radiation

Go Intensity of Incident Radiation = Intensity of Transmitted Radiation*10^(Absorbance)

Molar Extinction Coefficient given Slope of Plot

Go Molar Extinction Coefficient = Slope of Line/Thickness of Cell

Slope of Absorbance vs Concentration Plot

Go Slope of Line = Molar Extinction Coefficient*Thickness of Cell

Thickness of Cell given Slope

Go Thickness of Cell = Slope of Line/Molar Extinction Coefficient

Slope of Absorbance vs Concentration Plot Formula

Slope of Line = Molar Extinction Coefficient*Thickness of Cell
m = ε*l

What is Beer- Lambert Law?

Beer-Lambert law is useful in calculating the concentration of a solution on the basis of its light absorption. This law relates the intensity of the transmitted monochromatic light to the concentration of the solution and the thickness of the cell in which the solution is kept.
The molar extinction coefficient of a substance can be determined using a colorimeter or a spectrophotometer as follows. The absorbances of a solution are measured at different
known concentrations using a cell of known thickness (l). The plot of absorbance, A against Concentration of solution, c gives a straight line and its slope is equal to εl.

How to Calculate Slope of Absorbance vs Concentration Plot?

Slope of Absorbance vs Concentration Plot calculator uses Slope of Line = Molar Extinction Coefficient*Thickness of Cell to calculate the Slope of Line, The Slope of absorbance vs concentration plot formula is defined as a number that measures its "steepness", usually denoted by the letter m. It is the change in y for a unit change in x along the line. Slope of Line is denoted by m symbol.

How to calculate Slope of Absorbance vs Concentration Plot using this online calculator? To use this online calculator for Slope of Absorbance vs Concentration Plot, enter Molar Extinction Coefficient (ε) & Thickness of Cell (l) and hit the calculate button. Here is how the Slope of Absorbance vs Concentration Plot calculation can be explained with given input values -> 9.6E-11 = 0.0019*5.05E-08.

FAQ

What is Slope of Absorbance vs Concentration Plot?

The Slope of absorbance vs concentration plot formula is defined as a number that measures its "steepness", usually denoted by the letter m. It is the change in y for a unit change in x along the line and is represented as m = ε*l or Slope of Line = Molar Extinction Coefficient*Thickness of Cell. The Molar Extinction Coefficient is a measure of how strongly a chemical species or substance absorbs light at a particular wavelength & Thickness of Cell is useful in calculating the concentration of a solution on the basis of its light absorption.

How to calculate Slope of Absorbance vs Concentration Plot?

The Slope of absorbance vs concentration plot formula is defined as a number that measures its "steepness", usually denoted by the letter m. It is the change in y for a unit change in x along the line is calculated using Slope of Line = Molar Extinction Coefficient*Thickness of Cell. To calculate Slope of Absorbance vs Concentration Plot, you need Molar Extinction Coefficient (ε) & Thickness of Cell (l). With our tool, you need to enter the respective value for Molar Extinction Coefficient & Thickness of Cell and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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