Substrate Concentration given Dissociation Rate Constant Calculator

✖The Dissociation Rate Constant is the ratio of reverse and forward rate constant.ⓘ Dissociation Rate Constant [K_D]			+10% -10%
✖The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.ⓘ Enzyme Substrate Complex Concentration [ES]			+10% -10%
✖The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.ⓘ Initial Enzyme Concentration [[E₀]]			+10% -10%

✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration given Dissociation Rate Constant [S]

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Formula

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Substrate Concentration given Dissociation Rate Constant

Formula

`"S" = ("K"_{"D"}*"ES")/(("[E"_{"0"}"]")-"ES")`

Example

`"0.633333mol/L"=("5.7mol/L"*"10mol/L")/("100mol/L"-"10mol/L")`

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Substrate Concentration given Dissociation Rate Constant Solution

STEP 0: Pre-Calculation Summary

Formula Used

Substrate Concentration = (Dissociation Rate Constant*Enzyme Substrate Complex Concentration)/(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)
S = (K_D*ES)/([E₀]-ES)
This formula uses 4 Variables

Variables Used

Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Dissociation Rate Constant - (Measured in Mole per Cubic Meter) - The Dissociation Rate Constant is the ratio of reverse and forward rate constant.
Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.

STEP 1: Convert Input(s) to Base Unit

Dissociation Rate Constant: 5.7 Mole per Liter --> 5700 Mole per Cubic Meter (Check conversion here)
Enzyme Substrate Complex Concentration: 10 Mole per Liter --> 10000 Mole per Cubic Meter (Check conversion here)
Initial Enzyme Concentration: 100 Mole per Liter --> 100000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

S = (K_D*ES)/([E₀]-ES) --> (5700*10000)/(100000-10000)

Evaluating ... ...

S = 633.333333333333

STEP 3: Convert Result to Output's Unit

633.333333333333 Mole per Cubic Meter -->0.633333333333333 Mole per Liter (Check conversion here)

FINAL ANSWER

0.633333333333333 ≈ 0.633333 Mole per Liter <-- Substrate Concentration

(Calculation completed in 00.020 seconds)

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< 21 Complex Concentration Calculators

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Go Initial Enzyme Concentration = ((Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration))+Enzyme Substrate Complex Concentration

Substrate Concentration in Enzymatic Reaction Mechanism

Go Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))

Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants

Go Catalyst Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration)

Substrate Concentration given Forward, Reverse, and Catalytic Rate Constants

Go Substrate Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Catalyst Concentration)

Initial Enzyme Concentration given Catalytic Rate Constant and Dissociation Rate Constants

Go Initial Enzyme Concentration = (Initial Reaction Rate*(Dissociation Rate Constant+Substrate Concentration))/(Catalytic Rate Constant*Substrate Concentration)

Substrate Concentration given Catalytic Rate Constant and Dissociation Rate Constants

Go Substrate Concentration = (Dissociation Rate Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Substrate Concentration given Catalytic Rate Constant and Initial Enzyme Concentration

Go Concentration of Substrate = (Michaelis Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Substrate Concentration given Dissociation Rate Constant

Go Substrate Concentration = (Dissociation Rate Constant*Enzyme Substrate Complex Concentration)/(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)

Initial Enzyme Concentration given Dissociation Rate Constant

Go Enzyme Concentration Initially = (Enzyme Substrate Complex Concentration*(Dissociation Rate Constant+Substrate Concentration))/(Substrate Concentration)

Enzyme Substrate Complex Concentration given Dissociation Rate Constant

Go Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)

Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration

Go Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration)

Initial Enzyme Concentration at Low Substrate Concentration

Go Initial Enzyme Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Substrate Concentration)

Inhibitor Concentration given Apparent Initial Enzyme Concentration

Go Inhibitor Concentration for CI = ((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1)*Enzyme Inhibitor Dissociation Constant

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Go Inhibitor Concentration = (Enzyme Substrate Modifying Factor-1)*Enzyme Substrate Dissociation Constant

Inhibitor Concentration given Enzyme Substrate Modifying Factor

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Inhibitor Concentration given Modifying Factor of Enzyme

Go Inhibitor Concentration = (Enzyme Modifying Factor-1)*Enzyme Inhibitor Dissociation Constant

Enzyme Substrate Complex Concentration given Rate Constant and Initial Rate

Go Enzyme Inhibitor Complex Concentration = (Initial Reaction Rate/Final Rate Constant)

Initial Enzyme Concentration given Rate Constant and Maximum Rate

Go Initial Enzyme Concentration = Maximum Rate/Final Rate Constant

Substrate Concentration given Dissociation Rate Constant Formula

Substrate Concentration = (Dissociation Rate Constant*Enzyme Substrate Complex Concentration)/(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)
S = (K_D*ES)/([E₀]-ES)

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Substrate Concentration given Dissociation Rate Constant?

Substrate Concentration given Dissociation Rate Constant calculator uses Substrate Concentration = (Dissociation Rate Constant*Enzyme Substrate Complex Concentration)/(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration) to calculate the Substrate Concentration, The Substrate concentration given dissociation rate constant formula is defined as the relation with the dissociation rate constant and the concentration of enzyme and substrate. Substrate Concentration is denoted by S symbol.

How to calculate Substrate Concentration given Dissociation Rate Constant using this online calculator? To use this online calculator for Substrate Concentration given Dissociation Rate Constant, enter Dissociation Rate Constant (K_D), Enzyme Substrate Complex Concentration (ES) & Initial Enzyme Concentration ([E₀]) and hit the calculate button. Here is how the Substrate Concentration given Dissociation Rate Constant calculation can be explained with given input values -> 0.000633 = (5700*10000)/(100000-10000).

FAQ

What is Substrate Concentration given Dissociation Rate Constant?

The Substrate concentration given dissociation rate constant formula is defined as the relation with the dissociation rate constant and the concentration of enzyme and substrate and is represented as S = (K_D*ES)/([E₀]-ES) or Substrate Concentration = (Dissociation Rate Constant*Enzyme Substrate Complex Concentration)/(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration). The Dissociation Rate Constant is the ratio of reverse and forward rate constant, The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate & The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.

How to calculate Substrate Concentration given Dissociation Rate Constant?

The Substrate concentration given dissociation rate constant formula is defined as the relation with the dissociation rate constant and the concentration of enzyme and substrate is calculated using Substrate Concentration = (Dissociation Rate Constant*Enzyme Substrate Complex Concentration)/(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration). To calculate Substrate Concentration given Dissociation Rate Constant, you need Dissociation Rate Constant (K_D), Enzyme Substrate Complex Concentration (ES) & Initial Enzyme Concentration ([E₀]). With our tool, you need to enter the respective value for Dissociation Rate Constant, Enzyme Substrate Complex Concentration & Initial Enzyme Concentration and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Substrate Concentration?

In this formula, Substrate Concentration uses Dissociation Rate Constant, Enzyme Substrate Complex Concentration & Initial Enzyme Concentration. We can use 6 other way(s) to calculate the same, which is/are as follows -

Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration)
Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/Maximum Rate
Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))
Substrate Concentration = (Dissociation Rate Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)
Substrate Concentration = (Dissociation Rate Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)
Substrate Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Catalyst Concentration)

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