Column Efficiency in Chromatography Calculator

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✖The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column.ⓘ Retention Volume [V_R]			+10% -10%
✖Width of Chromatographic Peak is the distance between points where lines tangent to the peak's left and right inflection points intersect the baseline.ⓘ Width of Chromatographic Peak [W_b]			+10% -10%

✖Column Efficiency, also known as plate count, is a measure of the dispersion of a peak.ⓘ Column Efficiency in Chromatography [N]

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Formula

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Column Efficiency in Chromatography

Formula

`"N" = 16*(("V"_{"R"}/ "W"_{"b"})^2)`

Example

`"3.211264"=16*(("11.2L"/ "25mm")^2)`

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Column Efficiency in Chromatography Solution

STEP 0: Pre-Calculation Summary

Formula Used

Column Efficiency = 16*((Retention Volume/ Width of Chromatographic Peak)^2)
N = 16*((V_R/ W_b)^2)
This formula uses 3 Variables

Variables Used

Column Efficiency - Column Efficiency, also known as plate count, is a measure of the dispersion of a peak.
Retention Volume - (Measured in Cubic Meter) - The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column.
Width of Chromatographic Peak - (Measured in Meter) - Width of Chromatographic Peak is the distance between points where lines tangent to the peak's left and right inflection points intersect the baseline.

STEP 1: Convert Input(s) to Base Unit

Retention Volume: 11.2 Liter --> 0.0112 Cubic Meter (Check conversion here)
Width of Chromatographic Peak: 25 Millimeter --> 0.025 Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

N = 16*((V_R/ W_b)^2) --> 16*((0.0112/ 0.025)^2)

Evaluating ... ...

N = 3.211264

STEP 3: Convert Result to Output's Unit

3.211264 --> No Conversion Required

FINAL ANSWER

3.211264 <-- Column Efficiency

(Calculation completed in 00.004 seconds)

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Created by Soupayan banerjee

National University of Judicial Science (NUJS), Kolkata

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Amity Institute Of Applied Sciences (AIAS, Amity University), Noida, India

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Column Efficiency in Chromatography Formula

Column Efficiency = 16*((Retention Volume/ Width of Chromatographic Peak)^2)
N = 16*((V_R/ W_b)^2)

What is Column Chromatography?

Column chromatography in chemistry is a chromatography method used to isolate a single chemical compound from a mixture. Chromatography is able to separate substances based on differential adsorption of compounds to the adsorbent; compounds move through the column at different rates, allowing them to be separated into fractions. The technique is widely applicable, as many different adsorbents (normal phase, reversed phase, or otherwise) can be used with a wide range of solvents.

How to Calculate Column Efficiency in Chromatography?

Column Efficiency in Chromatography calculator uses Column Efficiency = 16*((Retention Volume/ Width of Chromatographic Peak)^2) to calculate the Column Efficiency, The Column Efficiency in Chromatography formula is defined as a measure of the dispersion of a peak. Narrow peaks take up less space in the chromatogram and thus allow more peaks to be separated. Column Efficiency is denoted by N symbol.

How to calculate Column Efficiency in Chromatography using this online calculator? To use this online calculator for Column Efficiency in Chromatography, enter Retention Volume (V_R) & Width of Chromatographic Peak (W_b) and hit the calculate button. Here is how the Column Efficiency in Chromatography calculation can be explained with given input values -> 3.211264 = 16*((0.0112/ 0.025)^2).

FAQ

What is Column Efficiency in Chromatography?

The Column Efficiency in Chromatography formula is defined as a measure of the dispersion of a peak. Narrow peaks take up less space in the chromatogram and thus allow more peaks to be separated and is represented as N = 16*((V_R/ W_b)^2) or Column Efficiency = 16*((Retention Volume/ Width of Chromatographic Peak)^2). The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column & Width of Chromatographic Peak is the distance between points where lines tangent to the peak's left and right inflection points intersect the baseline.

How to calculate Column Efficiency in Chromatography?

The Column Efficiency in Chromatography formula is defined as a measure of the dispersion of a peak. Narrow peaks take up less space in the chromatogram and thus allow more peaks to be separated is calculated using Column Efficiency = 16*((Retention Volume/ Width of Chromatographic Peak)^2). To calculate Column Efficiency in Chromatography, you need Retention Volume (V_R) & Width of Chromatographic Peak (W_b). With our tool, you need to enter the respective value for Retention Volume & Width of Chromatographic Peak and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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