What is competitive Inhibition?
In competitive inhibition, the substrate and inhibitor cannot bind to the enzyme at the same time, as shown in the figure on the right. This usually results from the inhibitor having an affinity for the active site of an enzyme where the substrate also binds; the substrate and inhibitor compete for access to the enzyme's active site. This type of inhibition can be overcome by sufficiently high concentrations of substrate (Vmax remains constant), i.e., by out-competing the inhibitor. However, the apparent Km will increase as it takes a higher concentration of the substrate to reach the Km point, or half the Vmax. Competitive inhibitors are often similar in structure to the real substrate.
How to Calculate Concentration of Enzyme Catalyst in Presence of Inhibitor by Enzyme Conservation Law?
Concentration of Enzyme Catalyst in Presence of Inhibitor by Enzyme Conservation Law calculator uses Catalyst Concentration = (Initial Enzyme Concentration-Enzyme Substrate Complex Concentration-Enzyme Inhibitor Complex Concentration) to calculate the Catalyst Concentration, The Concentration of enzyme catalyst in presence of inhibitor by enzyme conservation law formula is defined as the subtraction of enzyme-substrate and enzyme-inhibitor complex concentration from the initial enzyme concentration of the system. Catalyst Concentration is denoted by E symbol.
How to calculate Concentration of Enzyme Catalyst in Presence of Inhibitor by Enzyme Conservation Law using this online calculator? To use this online calculator for Concentration of Enzyme Catalyst in Presence of Inhibitor by Enzyme Conservation Law, enter Initial Enzyme Concentration ([E0]), Enzyme Substrate Complex Concentration (ES) & Enzyme Inhibitor Complex Concentration (EI) and hit the calculate button. Here is how the Concentration of Enzyme Catalyst in Presence of Inhibitor by Enzyme Conservation Law calculation can be explained with given input values -> 0.061 = (100000-10000-29000).