Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime Solution

STEP 0: Pre-Calculation Summary
Formula Used
Efficiency of Energy Transfer = Rate of Energy Transfer/(1/Donor Lifetime with FRET)
E = KT/(1/ζDA)
This formula uses 3 Variables
Variables Used
Efficiency of Energy Transfer - The Efficiency of Energy Transfer describes the efficiency of energy transfer between two light-sensitive molecules (chromophores).
Rate of Energy Transfer - Rate of Energy Transfer is simply the rate of energy transfer from a donor to an acceptor.
Donor Lifetime with FRET - The Donor Lifetime with FRET is the donor fluorescence lifetimes in the presence of an acceptor.
STEP 1: Convert Input(s) to Base Unit
Rate of Energy Transfer: 0.01 --> No Conversion Required
Donor Lifetime with FRET: 0.004 --> No Conversion Required
STEP 2: Evaluate Formula
Substituting Input Values in Formula
E = KT/(1/ζDA) --> 0.01/(1/0.004)
Evaluating ... ...
E = 4E-05
STEP 3: Convert Result to Output's Unit
4E-05 --> No Conversion Required
FINAL ANSWER
4E-05 4E-5 <-- Efficiency of Energy Transfer
(Calculation completed in 00.004 seconds)

Credits

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Created by Abhijit gharphalia
national institute of technology meghalaya (NIT Meghalaya), Shillong
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National University of Judicial Science (NUJS), Kolkata
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11 Förster resonance energy transfer Calculators

Forster Critical Distance
​ Go Forster Critical Distance = 0.0211*((Refractive Index of Medium)^(-4)*(Fluorescence Quantum Yield without FRET)*(Orientation Factor)*(Spectral Overlap Integral))^(1/6)
Efficiency of Energy Transfer using Rate of Energy Transfer
​ Go Efficiency of Energy Transfer = Rate of Energy Transfer/(Rate of Energy Transfer+Rate of Non radiative Transitions+Rate of Radiative Transitions)
Donor Lifetime with FRET using Rate of Energy and Transitions
​ Go Donor Lifetime with FRET = 1/(Rate of Energy Transfer+Rate of Radiative Transitions+Rate of Non radiative Transitions)
Rate of Energy Transfer using Distances and Donor Lifetime
​ Go Rate of Energy Transfer = (1/Donor Lifetime)*(Forster Critical Distance/Donor to Acceptor Distance)^6
Efficiency of Energy Transfer using Photobleaching Decay Time Constant
​ Go Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET)
Efficiency of Energy Transfer using Distances
​ Go Efficiency of Energy Transfer = 1/(1+(Donor to Acceptor Distance/Forster Critical Distance)^6)
Efficiency of Energy Transfer using Fluorescence Intensity of Donor
​ Go Efficiency of Energy Transfer = 1-(Fluorescence Intensity with FRET/Fluorescence intensity)
Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime
​ Go Efficiency of Energy Transfer = Rate of Energy Transfer/(1/Donor Lifetime with FRET)
Donor Lifetime using Rates of Transitions
​ Go Donor Lifetime = 1/(Rate of Radiative Transitions+Rate of Non radiative Transitions)
Fluorescence Quantum Yield in FRET
​ Go Fluorescence Quantum Yield = Number of Photons Emitted/Number of Photons Absorbed
Efficiency of Energy Transfer using Donor Lifetime
​ Go Efficiency of Energy Transfer = 1-(Donor Lifetime with FRET/Donor Lifetime)

Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime Formula

Efficiency of Energy Transfer = Rate of Energy Transfer/(1/Donor Lifetime with FRET)
E = KT/(1/ζDA)

What is FRET (Förster resonance energy transfer)?

Förster resonance energy transfer (FRET), fluorescence resonance energy transfer, resonance energy transfer (RET) or electronic energy transfer (EET) is a mechanism describing energy transfer between two light-sensitive molecules (chromophores).

How to Calculate Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime?

Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime calculator uses Efficiency of Energy Transfer = Rate of Energy Transfer/(1/Donor Lifetime with FRET) to calculate the Efficiency of Energy Transfer, The Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime formula is defined as the quantum yield of the energy-transfer transition, i.e. the probability of energy-transfer event occurring per donor excitation event. It can be calculated as the multiplication of rate of energy transfer and the donor lifetime in presence of acceptor. Efficiency of Energy Transfer is denoted by E symbol.

How to calculate Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime using this online calculator? To use this online calculator for Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime, enter Rate of Energy Transfer (KT) & Donor Lifetime with FRET DA) and hit the calculate button. Here is how the Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime calculation can be explained with given input values -> 4E-5 = 0.01/(1/0.004).

FAQ

What is Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime?
The Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime formula is defined as the quantum yield of the energy-transfer transition, i.e. the probability of energy-transfer event occurring per donor excitation event. It can be calculated as the multiplication of rate of energy transfer and the donor lifetime in presence of acceptor and is represented as E = KT/(1/ζDA) or Efficiency of Energy Transfer = Rate of Energy Transfer/(1/Donor Lifetime with FRET). Rate of Energy Transfer is simply the rate of energy transfer from a donor to an acceptor & The Donor Lifetime with FRET is the donor fluorescence lifetimes in the presence of an acceptor.
How to calculate Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime?
The Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime formula is defined as the quantum yield of the energy-transfer transition, i.e. the probability of energy-transfer event occurring per donor excitation event. It can be calculated as the multiplication of rate of energy transfer and the donor lifetime in presence of acceptor is calculated using Efficiency of Energy Transfer = Rate of Energy Transfer/(1/Donor Lifetime with FRET). To calculate Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime, you need Rate of Energy Transfer (KT) & Donor Lifetime with FRET DA). With our tool, you need to enter the respective value for Rate of Energy Transfer & Donor Lifetime with FRET and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
How many ways are there to calculate Efficiency of Energy Transfer?
In this formula, Efficiency of Energy Transfer uses Rate of Energy Transfer & Donor Lifetime with FRET. We can use 5 other way(s) to calculate the same, which is/are as follows -
  • Efficiency of Energy Transfer = 1/(1+(Donor to Acceptor Distance/Forster Critical Distance)^6)
  • Efficiency of Energy Transfer = Rate of Energy Transfer/(Rate of Energy Transfer+Rate of Non radiative Transitions+Rate of Radiative Transitions)
  • Efficiency of Energy Transfer = 1-(Donor Lifetime with FRET/Donor Lifetime)
  • Efficiency of Energy Transfer = 1-(Fluorescence Intensity with FRET/Fluorescence intensity)
  • Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET)
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