How to Calculate Forster Critical Distance?
Forster Critical Distance calculator uses Forster Critical Distance = 0.0211*((Refractive Index of Medium)^(-4)*(Fluorescence Quantum Yield without FRET)*(Orientation Factor)*(Spectral Overlap Integral))^(1/6) to calculate the Forster Critical Distance, The Forster Critical Distance formula is defined as the Förster distance of this pair of donor and acceptor, i.e. the distance at which the energy transfer efficiency is 50%. Forster Critical Distance is denoted by R_{0} symbol.
How to calculate Forster Critical Distance using this online calculator? To use this online calculator for Forster Critical Distance, enter Refractive Index of Medium (η), Fluorescence Quantum Yield without FRET (Φ_{d}), Orientation Factor (κ^{2}) & Spectral Overlap Integral (J) and hit the calculate button. Here is how the Forster Critical Distance calculation can be explained with given input values -> 3.8E+17 = 0.0211*((1.3)^(-4)*(0.13)*(1.5)*(0.0005))^(1/6).