Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants Calculator

✖The Reverse Rate Constant is defined as the rate constant for the backward reaction.ⓘ Reverse Rate Constant [k_r]			+10% -10%
✖The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.ⓘ Catalytic Rate Constant [k_cat]			+10% -10%
✖The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.ⓘ Enzyme Substrate Complex Concentration [ES]			+10% -10%
✖The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.ⓘ Forward Rate Constant [k_f]			+10% -10%
✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration [S]			+10% -10%

✖The Catalyst Concentration is the number of moles of catalyst present in the per liter of solution.ⓘ Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants [E]

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Formula

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Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants

Formula

`"E" = (("k"_{"r"}+"k"_{"cat"})*"ES")/("k"_{"f"}*"S")`

Example

`"19.3243mol/L"=(("20mol/L*s"+"0.65s⁻¹")*"10mol/L")/("6.9s⁻¹"*"1.5mol/L")`

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Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants Solution

STEP 0: Pre-Calculation Summary

Formula Used

Catalyst Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration)
E = ((k_r+k_cat)*ES)/(k_f*S)
This formula uses 6 Variables

Variables Used

Catalyst Concentration - (Measured in Mole per Cubic Meter) - The Catalyst Concentration is the number of moles of catalyst present in the per liter of solution.
Reverse Rate Constant - (Measured in Mole per Cubic Meter Second) - The Reverse Rate Constant is defined as the rate constant for the backward reaction.
Catalytic Rate Constant - (Measured in 1 Per Second) - The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.
Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Forward Rate Constant - (Measured in 1 Per Second) - The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.

STEP 1: Convert Input(s) to Base Unit

Reverse Rate Constant: 20 Mole per Liter Second --> 20000 Mole per Cubic Meter Second (Check conversion here)
Catalytic Rate Constant: 0.65 1 Per Second --> 0.65 1 Per Second No Conversion Required
Enzyme Substrate Complex Concentration: 10 Mole per Liter --> 10000 Mole per Cubic Meter (Check conversion here)
Forward Rate Constant: 6.9 1 Per Second --> 6.9 1 Per Second No Conversion Required
Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

E = ((k_r+k_cat)*ES)/(k_f*S) --> ((20000+0.65)*10000)/(6.9*1500)

Evaluating ... ...

E = 19324.2995169082

STEP 3: Convert Result to Output's Unit

19324.2995169082 Mole per Cubic Meter -->19.3242995169082 Mole per Liter (Check conversion here)

FINAL ANSWER

19.3242995169082 ≈ 19.3243 Mole per Liter <-- Catalyst Concentration

(Calculation completed in 00.004 seconds)

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< 21 Complex Concentration Calculators

Enzyme Substrate Complex Concentration in Instantaneous Chemical Equilibrium

Go Enzyme Substrate Complex Concentration = (Forward Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/(Reverse Rate Constant+(Forward Rate Constant*Substrate Concentration))

Initial Enzyme Concentration in Enzymatic Reaction Mechanism

Go Initial Enzyme Concentration = ((Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration))+Enzyme Substrate Complex Concentration

Substrate Concentration in Enzymatic Reaction Mechanism

Go Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))

Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants

Go Catalyst Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration)

Substrate Concentration given Forward, Reverse, and Catalytic Rate Constants

Go Substrate Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Catalyst Concentration)

Initial Enzyme Concentration given Catalytic Rate Constant and Dissociation Rate Constants

Go Initial Enzyme Concentration = (Initial Reaction Rate*(Dissociation Rate Constant+Substrate Concentration))/(Catalytic Rate Constant*Substrate Concentration)

Substrate Concentration given Catalytic Rate Constant and Dissociation Rate Constants

Go Substrate Concentration = (Dissociation Rate Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Substrate Concentration given Catalytic Rate Constant and Initial Enzyme Concentration

Go Concentration of Substrate = (Michaelis Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Substrate Concentration given Dissociation Rate Constant

Go Substrate Concentration = (Dissociation Rate Constant*Enzyme Substrate Complex Concentration)/(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)

Initial Enzyme Concentration given Dissociation Rate Constant

Go Enzyme Concentration Initially = (Enzyme Substrate Complex Concentration*(Dissociation Rate Constant+Substrate Concentration))/(Substrate Concentration)

Enzyme Substrate Complex Concentration given Dissociation Rate Constant

Go Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)

Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration

Go Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration)

Initial Enzyme Concentration at Low Substrate Concentration

Go Initial Enzyme Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Substrate Concentration)

Inhibitor Concentration given Apparent Initial Enzyme Concentration

Go Inhibitor Concentration for CI = ((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1)*Enzyme Inhibitor Dissociation Constant

Substrate Concentration given Maximum Rate and Dissociation Rate Constant

Go Substrate Concentration = (Dissociation Rate Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)

Substrate Concentration given Maximum Rate at Low Concentration

Go Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/Maximum Rate

Inhibitor Concentration given Modifying Factor of Enzyme Substrate Complex

Go Inhibitor Concentration = (Enzyme Substrate Modifying Factor-1)*Enzyme Substrate Dissociation Constant

Inhibitor Concentration given Enzyme Substrate Modifying Factor

Go Inhibitor Concentration = (Enzyme Substrate Modifying Factor-1)*Enzyme Substrate Dissociation Constant

Inhibitor Concentration given Modifying Factor of Enzyme

Go Inhibitor Concentration = (Enzyme Modifying Factor-1)*Enzyme Inhibitor Dissociation Constant

Enzyme Substrate Complex Concentration given Rate Constant and Initial Rate

Go Enzyme Inhibitor Complex Concentration = (Initial Reaction Rate/Final Rate Constant)

Initial Enzyme Concentration given Rate Constant and Maximum Rate

Go Initial Enzyme Concentration = Maximum Rate/Final Rate Constant

< 25 Important Formulas on Enzyme Kinetics Calculators

Final Rate Constant for Competitive Inhibition of Enzyme Catalysis

Go Final Rate Constant for Catalysis = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Initial Enzyme Concentration*Substrate Concentration)

Inhibitor Concentration for Competitive Inhibition of Enzyme Catalysis

Go Inhibitor Concentration given IEC = (((((Final Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant

Michaelis Constant in Competitive Inhibition given Enzyme Substrate Complex Concentration

Go Michaelis Constant = (((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))

Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis

Go Enzyme Substrate Complex Concentration = (Substrate Concentration*Initial Enzyme Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration)

Inhibitor Concentration in Competitive Inhibition given Maximum Rate of System

Go Inhibitor Concentration given Max Rate = (((((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant

Initial Rate in Competitive Inhibition given Maximum Rate of system

Go Initial Reaction Rate in CI = (Maximum Rate*Substrate Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration)

Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants

Go Catalyst Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration)

Catalytic Rate Constant from Michaelis Menten Kinetics Equation

Go Catalytic Rate Constant for MM = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/(Initial Enzyme Concentration*Substrate Concentration)

Enzyme Concentration from Michaelis Menten Kinetics equation

Go Initial Concentration of Enzyme = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/(Catalytic Rate Constant*Substrate Concentration)

Substrate Concentration given Catalytic Rate Constant and Initial Enzyme Concentration

Go Concentration of Substrate = (Michaelis Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Initial Enzyme Concentration given Dissociation Rate Constant

Go Enzyme Concentration Initially = (Enzyme Substrate Complex Concentration*(Dissociation Rate Constant+Substrate Concentration))/(Substrate Concentration)

Initial Reaction Rate given Dissociation Rate Constant

Go Initial Reaction Rate given DRC = (Maximum Rate*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)

Maximum Rate given Dissociation Rate Constant

Go Maximum Rate given DRC = (Initial Reaction Rate*(Dissociation Rate Constant+Substrate Concentration))/Substrate Concentration

Inhibitor Concentration given Apparent Initial Enzyme Concentration

Go Inhibitor Concentration for CI = ((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1)*Enzyme Inhibitor Dissociation Constant

Initial Concentration of Enzyme in presence of Inhibitor by Enzyme Conservation Law

Go Enzyme Concentration Initially = (Catalyst Concentration+Enzyme Substrate Complex Concentration+Enzyme Inhibitor Complex Concentration)

Michaelis Constant given Forward, Reverse, and Catalytic Rate Constants

Go Michaelis Constant = (Reverse Rate Constant+Catalytic Rate Constant)/Forward Rate Constant

Inhibitor Concentration given Enzyme Substrate Modifying Factor

Go Inhibitor Concentration = (Enzyme Substrate Modifying Factor-1)*Enzyme Substrate Dissociation Constant

Modifying Factor of Enzyme Substrate Complex

Go Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant)

Dissociation Constant of Enzyme given Modifying Factor of Enzyme

Go Enzyme Inhibitor Dissociation Constant given MF = Inhibitor Concentration/(Enzyme Modifying Factor-1)

Initial Rate of System given Rate Constant and Enzyme Substrate Complex Concentration

Go Initial Reaction Rate given RC = Final Rate Constant*Enzyme Substrate Complex Concentration

Forward Rate Constant given Dissociation Rate Constant

Go Forward Rate Constant = (Reverse Rate Constant/Dissociation Rate Constant)

Dissociation Rate Constant in Enzymatic Reaction Mechanism

Go Dissociation Rate Constant = Reverse Rate Constant/Forward Rate Constant

Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant

Go Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant

Catalytic Rate Constant if Substrate Concentration is higher than Michaelis Constant

Go Catalytic Rate Constant = Maximum Rate/Initial Enzyme Concentration

Maximum Rate if Substrate Concentration is Higher than Michaelis Constant

Go Maximum Rate = Catalytic Rate Constant*Initial Enzyme Concentration

Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants Formula

Catalyst Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration)
E = ((k_r+k_cat)*ES)/(k_f*S)

What is catalytic rate constant?

The catalytic rate constants, with units of s-1 is often called the turnover number. It is a measure of how many bound substrate molecules turnover or form product in 1 second. This is evident from equation v0 = kcat[ES].

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants?

Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants calculator uses Catalyst Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration) to calculate the Catalyst Concentration, The Enzyme catalyst concentration given forward, reverse, and catalytic rate constants formula is defined as the relation with substrate concentration and with an enzyme-substrate complex concentration in an enzymatic reaction mechanism. Catalyst Concentration is denoted by E symbol.

How to calculate Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants using this online calculator? To use this online calculator for Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants, enter Reverse Rate Constant (k_r), Catalytic Rate Constant (k_cat), Enzyme Substrate Complex Concentration (ES), Forward Rate Constant (k_f) & Substrate Concentration (S) and hit the calculate button. Here is how the Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants calculation can be explained with given input values -> 0.019324 = ((20000+0.65)*10000)/(6.9*1500).

FAQ

What is Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants?

The Enzyme catalyst concentration given forward, reverse, and catalytic rate constants formula is defined as the relation with substrate concentration and with an enzyme-substrate complex concentration in an enzymatic reaction mechanism and is represented as E = ((k_r+k_cat)*ES)/(k_f*S) or Catalyst Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration). The Reverse Rate Constant is defined as the rate constant for the backward reaction, The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product, The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate, The Forward Rate Constant is defined as the rate constant for the forward occurring reaction & The Substrate Concentration is the number of moles of substrate per liter solution.

How to calculate Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants?

The Enzyme catalyst concentration given forward, reverse, and catalytic rate constants formula is defined as the relation with substrate concentration and with an enzyme-substrate complex concentration in an enzymatic reaction mechanism is calculated using Catalyst Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration). To calculate Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants, you need Reverse Rate Constant (k_r), Catalytic Rate Constant (k_cat), Enzyme Substrate Complex Concentration (ES), Forward Rate Constant (k_f) & Substrate Concentration (S). With our tool, you need to enter the respective value for Reverse Rate Constant, Catalytic Rate Constant, Enzyme Substrate Complex Concentration, Forward Rate Constant & Substrate Concentration and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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