Enzyme Substrate Inhibitor Concentration in presence of Uncompetitive Inhibitor Calculator

✖The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.ⓘ Enzyme Substrate Complex Concentration [ES]			+10% -10%
✖The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.ⓘ Inhibitor Concentration [I]			+10% -10%
✖The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.ⓘ Enzyme Substrate Dissociation Constant [K_i']			+10% -10%

✖The Enzyme Substrate Inhibitor Complex Concentration is the molar concentration of the complex.ⓘ Enzyme Substrate Inhibitor Concentration in presence of Uncompetitive Inhibitor [ESI]

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Formula

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Enzyme Substrate Inhibitor Concentration in presence of Uncompetitive Inhibitor

Formula

`"ESI" = ("ES"*"I")/("K"_{"i"}"'")`

Example

`"6mol/L"=("10mol/L"*"9mol/L")/"15mol/L"`

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Enzyme Substrate Inhibitor Concentration in presence of Uncompetitive Inhibitor Solution

STEP 0: Pre-Calculation Summary

Formula Used

Enzyme Substrate Inhibitor Complex Concentration = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Dissociation Constant
ESI = (ES*I)/K_i'
This formula uses 4 Variables

Variables Used

Enzyme Substrate Inhibitor Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Inhibitor Complex Concentration is the molar concentration of the complex.
Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Enzyme Substrate Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.

STEP 1: Convert Input(s) to Base Unit

Enzyme Substrate Complex Concentration: 10 Mole per Liter --> 10000 Mole per Cubic Meter (Check conversion here)
Inhibitor Concentration: 9 Mole per Liter --> 9000 Mole per Cubic Meter (Check conversion here)
Enzyme Substrate Dissociation Constant: 15 Mole per Liter --> 15000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

ESI = (ES*I)/K_i' --> (10000*9000)/15000

Evaluating ... ...

ESI = 6000

STEP 3: Convert Result to Output's Unit

6000 Mole per Cubic Meter -->6 Mole per Liter (Check conversion here)

FINAL ANSWER

6 Mole per Liter <-- Enzyme Substrate Inhibitor Complex Concentration

(Calculation completed in 00.004 seconds)

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< 12 Uncompetitive Inhibitor Calculators

Enzyme Substrate Modifying Factor in Presence of Uncompetitive Inhibitor

Go Enzyme Substrate Modifying Factor = ((Maximum Rate*Substrate Concentration)-(Initial Reaction Rate*Michaelis Constant))/(Initial Reaction Rate*Substrate Concentration)

Enzyme Substate complex Concentration given Forward, Reverse, and Catalytic Rate Constant

Go Enzyme Substrate Complex Concentration = (Forward Rate Constant*Catalyst Concentration*Substrate Concentration)/(Reverse Rate Constant+Catalytic Rate Constant)

Initial Reaction Rate in presence of Uncompetitive Inhibitor

Go Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration))

Maximum Reaction Rate in Presence of Uncompetitive Inhibitor

Go Maximum Rate = (Initial Reaction Rate*(Michaelis Constant+(Enzyme Substrate Modifying Factor*Substrate Concentration)))/Substrate Concentration

Substrate Concentration in presence of Uncompetitive Inhibitor

Go Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor))

Michaelis Constant in Presence of Uncompetitive Inhibitor

Go Michaelis Constant = (Substrate Concentration*(Maximum Rate-(Initial Reaction Rate*Enzyme Substrate Modifying Factor)))/Initial Reaction Rate

Enzyme Substrate Inhibitor Concentration in presence of Uncompetitive Inhibitor

Go Enzyme Substrate Inhibitor Complex Concentration = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Dissociation Constant

Enzyme Substrate Dissociation constant in Presence of Uncompetitive Inhibitor

Go Enzyme Substrate Dissociation Constant = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Inhibitor Complex Concentration

Enzyme Substrate complex Concentration in Presence of Uncompetitive Inhibitor

Go Enzyme Substrate Complex Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Inhibitor Concentration

Inhibitor Concentration in Presence of Uncompetitive Inhibitor

Go Inhibitor Concentration = (Enzyme Substrate Dissociation Constant*Enzyme Substrate Inhibitor Complex Concentration)/Enzyme Substrate Complex Concentration

Enzyme Substrate Modifying Factor given Enzyme Substrate Dissociation Constant

Go Enzyme Substrate Modifying Factor = (1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant))

Enzyme Substrate Dissociation constant given Enzyme Substrate Modifying factor

Go Enzyme Substrate Dissociation Constant = Inhibitor Concentration/(Enzyme Substrate Modifying Factor-1)

Enzyme Substrate Inhibitor Concentration in presence of Uncompetitive Inhibitor Formula

Enzyme Substrate Inhibitor Complex Concentration = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Dissociation Constant
ESI = (ES*I)/K_i'

What is Uncompetitive inhibitor?

Uncompetitive inhibition, also known as anti-competitive inhibition, takes place when an enzyme inhibitor binds only to the complex formed between the enzyme and the substrate (the E-S complex). Uncompetitive inhibition typically occurs in reactions with two or more substrates or products. Uncompetitive inhibition is distinguished from competitive inhibition by two observations: first uncompetitive inhibition cannot be reversed by increasing [S] and second, as shown, the Lineweaver–Burk plot yields parallel rather than intersecting lines.

How to Calculate Enzyme Substrate Inhibitor Concentration in presence of Uncompetitive Inhibitor?

Enzyme Substrate Inhibitor Concentration in presence of Uncompetitive Inhibitor calculator uses Enzyme Substrate Inhibitor Complex Concentration = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Dissociation Constant to calculate the Enzyme Substrate Inhibitor Complex Concentration, The Enzyme substrate inhibitor concentration in presence of uncompetitive inhibitor formula is defined as the relation with the concentration of different enzyme species. Enzyme Substrate Inhibitor Complex Concentration is denoted by ESI symbol.

How to calculate Enzyme Substrate Inhibitor Concentration in presence of Uncompetitive Inhibitor using this online calculator? To use this online calculator for Enzyme Substrate Inhibitor Concentration in presence of Uncompetitive Inhibitor, enter Enzyme Substrate Complex Concentration (ES), Inhibitor Concentration (I) & Enzyme Substrate Dissociation Constant (K_i') and hit the calculate button. Here is how the Enzyme Substrate Inhibitor Concentration in presence of Uncompetitive Inhibitor calculation can be explained with given input values -> 0.006 = (10000*9000)/15000.

FAQ

What is Enzyme Substrate Inhibitor Concentration in presence of Uncompetitive Inhibitor?

The Enzyme substrate inhibitor concentration in presence of uncompetitive inhibitor formula is defined as the relation with the concentration of different enzyme species and is represented as ESI = (ES*I)/K_i' or Enzyme Substrate Inhibitor Complex Concentration = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Dissociation Constant. The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate, The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system & The Enzyme Substrate Dissociation Constant is difficult to measure directly since the enzyme-substrate complex is short-lived and undergoes a chemical reaction to form the product.

How to calculate Enzyme Substrate Inhibitor Concentration in presence of Uncompetitive Inhibitor?

The Enzyme substrate inhibitor concentration in presence of uncompetitive inhibitor formula is defined as the relation with the concentration of different enzyme species is calculated using Enzyme Substrate Inhibitor Complex Concentration = (Enzyme Substrate Complex Concentration*Inhibitor Concentration)/Enzyme Substrate Dissociation Constant. To calculate Enzyme Substrate Inhibitor Concentration in presence of Uncompetitive Inhibitor, you need Enzyme Substrate Complex Concentration (ES), Inhibitor Concentration (I) & Enzyme Substrate Dissociation Constant (K_i'). With our tool, you need to enter the respective value for Enzyme Substrate Complex Concentration, Inhibitor Concentration & Enzyme Substrate Dissociation Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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