Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant Calculator

✖The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.ⓘ Maximum Rate [V_max]			+10% -10%
✖The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.ⓘ Catalytic Rate Constant [k_cat]			+10% -10%

✖Enzyme Concentration Initially is defined as the concentration of enzyme at the start of the reaction.ⓘ Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant [[E_initial]]

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Formula

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Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant

Formula

`("[E"_{"initial"}"]") = "V"_{"max"}/"k"_{"cat"}`

Example

`"61.53846mol/L"="40mol/L*s"/"0.65s⁻¹"`

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Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant Solution

STEP 0: Pre-Calculation Summary

Formula Used

Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant
[E_initial] = V_max/k_cat
This formula uses 3 Variables

Variables Used

Enzyme Concentration Initially - (Measured in Mole per Cubic Meter) - Enzyme Concentration Initially is defined as the concentration of enzyme at the start of the reaction.
Maximum Rate - (Measured in Mole per Cubic Meter Second) - The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.
Catalytic Rate Constant - (Measured in 1 Per Second) - The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.

STEP 1: Convert Input(s) to Base Unit

Maximum Rate: 40 Mole per Liter Second --> 40000 Mole per Cubic Meter Second (Check conversion here)
Catalytic Rate Constant: 0.65 1 Per Second --> 0.65 1 Per Second No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

[E_initial] = V_max/k_cat --> 40000/0.65

Evaluating ... ...

[E_initial] = 61538.4615384615

STEP 3: Convert Result to Output's Unit

61538.4615384615 Mole per Cubic Meter -->61.5384615384615 Mole per Liter (Check conversion here)

FINAL ANSWER

61.5384615384615 ≈ 61.53846 Mole per Liter <-- Enzyme Concentration Initially

(Calculation completed in 00.004 seconds)

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< 25 Michaelis Menten Kinetics Equation Calculators

Michaelis Constant given Modifying Factor in Michaelis Menten Equation

Go Michaelis Constant = (Substrate Concentration*((1/Enzyme Substrate Modifying Factor)*Maximum Rate)-Initial Reaction Rate)/((Enzyme Modifying Factor/Enzyme Substrate Modifying Factor)*Substrate Concentration)

Initial Reaction Rate of Enzyme given Modifying factor in Michaelis Menten equation

Go Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/((Enzyme Modifying Factor*Michaelis Constant)+(Enzyme Substrate Modifying Factor*Substrate Concentration))

Modifying Factor of Enzyme Substrate Complex in Michaelis Menten Equation

Go Enzyme Substrate Modifying Factor = (((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-(Enzyme Modifying Factor*Michaelis Constant))/Substrate Concentration

Maximum Rate given Modifying Factor in Michaelis Menten Equation

Go Maximum Rate = (Initial Reaction Rate*((Enzyme Modifying Factor*Michaelis Constant)+(Enzyme Substrate Modifying Factor*Substrate Concentration)))/Substrate Concentration

Modifying Factor of Enzyme in Michaelis Menten Equation

Go Enzyme Modifying Factor = (((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-(Enzyme Substrate Modifying Factor*Substrate Concentration))/Michaelis Constant

Catalytic Rate Constant from Michaelis Menten Kinetics Equation

Go Catalytic Rate Constant for MM = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/(Initial Enzyme Concentration*Substrate Concentration)

Enzyme Concentration from Michaelis Menten Kinetics equation

Go Initial Concentration of Enzyme = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/(Catalytic Rate Constant*Substrate Concentration)

Michaelis Constant given Catalytic Rate Constant and Initial Enzyme Concentration

Go Michaelis Constant = (Substrate Concentration*((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate))/Initial Reaction Rate

Michaelis Constant at Low Substrate Concentration

Go Michaelis Constant = (Catalytic Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate

Dissociation Rate Constant from Michaelis Menten kinetics equation

Go Dissociation Rate Constant = ((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-(Substrate Concentration)

Initial Rate given Apparent value of Michaelis Menten Constant

Go Initial Reaction Rate = (Maximum Rate*Substrate Concentration)/(Apparent Michaelis Constant+Substrate Concentration)

Maximum Rate given Apparent Value of Michaelis Menten Constant

Go Maximum Rate = (Initial Reaction Rate*(Apparent Michaelis Constant+Substrate Concentration))/Substrate Concentration

Maximum Rate of System from Michaelis Menten Kinetics equation

Go Maximum Rate = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/Substrate Concentration

Substrate Concentration from Michaelis Menten Kinetics Equation

Go Substrate Concentration = (Michaelis Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)

Michaelis Constant from Michaelis Menten kinetics equation

Go Michaelis Constant = Substrate Concentration*((Maximum Rate-Initial Reaction Rate)/Initial Reaction Rate)

Inhibitor's Dissociation Constant given Michaelis Menten Constant

Go Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Apparent Michaelis Constant/Michaelis Constant)-1))

Michaelis Menten constant given Apparent Michaelis Menten Constant

Go Michaelis Constant = Apparent Michaelis Constant/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))

Inhibitor Concentration given Apparent Michaelis Menten Constant

Go Inhibitor Concentration = ((Apparent Michaelis Constant/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant

Michaelis Constant given Forward, Reverse, and Catalytic Rate Constants

Go Michaelis Constant = (Reverse Rate Constant+Catalytic Rate Constant)/Forward Rate Constant

Catalytic rate constant given Michaelis Constant

Go Catalytic Rate Constant = (Michaelis Constant*Forward Rate Constant)-Reverse Rate Constant

Forward Rate Constant given Michaelis Constant

Go Forward Rate Constant = (Reverse Rate Constant+Catalytic Rate Constant)/Michaelis Constant

Michaelis Constant given Maximum Rate at Low Substrate Concentration

Go Michaelis Constant = (Maximum Rate*Substrate Concentration)/Initial Reaction Rate

Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant

Go Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant

Catalytic Rate Constant if Substrate Concentration is higher than Michaelis Constant

Go Catalytic Rate Constant = Maximum Rate/Initial Enzyme Concentration

Maximum Rate if Substrate Concentration is Higher than Michaelis Constant

Go Maximum Rate = Catalytic Rate Constant*Initial Enzyme Concentration

< 25 Important Formulas on Enzyme Kinetics Calculators

Final Rate Constant for Competitive Inhibition of Enzyme Catalysis

Go Final Rate Constant for Catalysis = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Initial Enzyme Concentration*Substrate Concentration)

Inhibitor Concentration for Competitive Inhibition of Enzyme Catalysis

Go Inhibitor Concentration given IEC = (((((Final Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant

Michaelis Constant in Competitive Inhibition given Enzyme Substrate Complex Concentration

Go Michaelis Constant = (((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))

Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis

Go Enzyme Substrate Complex Concentration = (Substrate Concentration*Initial Enzyme Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration)

Inhibitor Concentration in Competitive Inhibition given Maximum Rate of System

Go Inhibitor Concentration given Max Rate = (((((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant

Initial Rate in Competitive Inhibition given Maximum Rate of system

Go Initial Reaction Rate in CI = (Maximum Rate*Substrate Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration)

Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants

Go Catalyst Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration)

Catalytic Rate Constant from Michaelis Menten Kinetics Equation

Go Catalytic Rate Constant for MM = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/(Initial Enzyme Concentration*Substrate Concentration)

Enzyme Concentration from Michaelis Menten Kinetics equation

Go Initial Concentration of Enzyme = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/(Catalytic Rate Constant*Substrate Concentration)

Substrate Concentration given Catalytic Rate Constant and Initial Enzyme Concentration

Go Concentration of Substrate = (Michaelis Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Initial Enzyme Concentration given Dissociation Rate Constant

Go Enzyme Concentration Initially = (Enzyme Substrate Complex Concentration*(Dissociation Rate Constant+Substrate Concentration))/(Substrate Concentration)

Initial Reaction Rate given Dissociation Rate Constant

Go Initial Reaction Rate given DRC = (Maximum Rate*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)

Maximum Rate given Dissociation Rate Constant

Go Maximum Rate given DRC = (Initial Reaction Rate*(Dissociation Rate Constant+Substrate Concentration))/Substrate Concentration

Inhibitor Concentration given Apparent Initial Enzyme Concentration

Go Inhibitor Concentration for CI = ((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1)*Enzyme Inhibitor Dissociation Constant

Initial Concentration of Enzyme in presence of Inhibitor by Enzyme Conservation Law

Go Enzyme Concentration Initially = (Catalyst Concentration+Enzyme Substrate Complex Concentration+Enzyme Inhibitor Complex Concentration)

Michaelis Constant given Forward, Reverse, and Catalytic Rate Constants

Go Michaelis Constant = (Reverse Rate Constant+Catalytic Rate Constant)/Forward Rate Constant

Inhibitor Concentration given Enzyme Substrate Modifying Factor

Go Inhibitor Concentration = (Enzyme Substrate Modifying Factor-1)*Enzyme Substrate Dissociation Constant

Modifying Factor of Enzyme Substrate Complex

Go Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant)

Dissociation Constant of Enzyme given Modifying Factor of Enzyme

Go Enzyme Inhibitor Dissociation Constant given MF = Inhibitor Concentration/(Enzyme Modifying Factor-1)

Initial Rate of System given Rate Constant and Enzyme Substrate Complex Concentration

Go Initial Reaction Rate given RC = Final Rate Constant*Enzyme Substrate Complex Concentration

Forward Rate Constant given Dissociation Rate Constant

Go Forward Rate Constant = (Reverse Rate Constant/Dissociation Rate Constant)

Dissociation Rate Constant in Enzymatic Reaction Mechanism

Go Dissociation Rate Constant = Reverse Rate Constant/Forward Rate Constant

Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant

Go Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant

Catalytic Rate Constant if Substrate Concentration is higher than Michaelis Constant

Go Catalytic Rate Constant = Maximum Rate/Initial Enzyme Concentration

Maximum Rate if Substrate Concentration is Higher than Michaelis Constant

Go Maximum Rate = Catalytic Rate Constant*Initial Enzyme Concentration

Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant Formula

Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant
[E_initial] = V_max/k_cat

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant?

Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant calculator uses Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant to calculate the Enzyme Concentration Initially, The Initial enzyme concentration if substrate concentration is higher than Michaelis constant formula is defined as the ratio of the maximum rate to the catalytic rate constant. Here substrate concentration is very large in comparison to Michaelis constant. Enzyme Concentration Initially is denoted by [E_initial] symbol.

How to calculate Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant using this online calculator? To use this online calculator for Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant, enter Maximum Rate (V_max) & Catalytic Rate Constant (k_cat) and hit the calculate button. Here is how the Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant calculation can be explained with given input values -> 0.061538 = 40000/0.65.

FAQ

What is Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant?

The Initial enzyme concentration if substrate concentration is higher than Michaelis constant formula is defined as the ratio of the maximum rate to the catalytic rate constant. Here substrate concentration is very large in comparison to Michaelis constant and is represented as [E_initial] = V_max/k_cat or Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant. The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration & The Catalytic Rate Constant is defined as the rate constant for conversion of the enzyme-substrate complex to enzyme and product.

How to calculate Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant?

The Initial enzyme concentration if substrate concentration is higher than Michaelis constant formula is defined as the ratio of the maximum rate to the catalytic rate constant. Here substrate concentration is very large in comparison to Michaelis constant is calculated using Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant. To calculate Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant, you need Maximum Rate (V_max) & Catalytic Rate Constant (k_cat). With our tool, you need to enter the respective value for Maximum Rate & Catalytic Rate Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Enzyme Concentration Initially?

In this formula, Enzyme Concentration Initially uses Maximum Rate & Catalytic Rate Constant. We can use 2 other way(s) to calculate the same, which is/are as follows -

Enzyme Concentration Initially = (Enzyme Substrate Complex Concentration*(Dissociation Rate Constant+Substrate Concentration))/(Substrate Concentration)
Enzyme Concentration Initially = (Catalyst Concentration+Enzyme Substrate Complex Concentration+Enzyme Inhibitor Complex Concentration)

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