Peak Resolution in Chromatography Calculator

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✖Retention Volume of Molecule 2 is the volume that has passed through the column since the target molecule 2 was introduced onto the column.ⓘ Retention Volume of Molecule 2 [V_R2]			+10% -10%
✖Retention Volume of Molecule 1 is the volume that has passed through the column since the target molecule 1 was introduced onto the column.ⓘ Retention Volume of Molecule 1 [V_R1]			+10% -10%
✖Width of Chromatographic Peak of Molecule 1 is the distance between points of molecule 1 where lines tangent to the peak's left and right inflection points intersect the baseline.ⓘ Width of Chromatographic Peak of Molecule 1 [W_b1]			+10% -10%
✖Widths of Chromatographic Peak of Molecule 2 is the distance between points of molecule 2 where lines tangent to the peak's left and right inflection points intersect the baseline.ⓘ Widths of Chromatographic Peak of Molecule 2 [W_b2]			+10% -10%

✖Peak Resolution is the difference in retention times between the two peaks, divided by the combined widths of the elution peaks.ⓘ Peak Resolution in Chromatography [R_s]

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Formula

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Peak Resolution in Chromatography

Formula

`"R"_{"s"} = ("V"_{"R2"}-"V"_{"R1"})/(("W"_{"b1"}+"W"_{"b2"})/2)`

Example

`"3.3E^-5"=("10.5mL"-"9.56mL")/(("30mm"+"27mm")/2)`

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Peak Resolution in Chromatography Solution

STEP 0: Pre-Calculation Summary

Formula Used

Peak Resolution = (Retention Volume of Molecule 2-Retention Volume of Molecule 1)/((Width of Chromatographic Peak of Molecule 1+Widths of Chromatographic Peak of Molecule 2)/2)
R_s = (V_R2-V_R1)/((W_b1+W_b2)/2)
This formula uses 5 Variables

Variables Used

Peak Resolution - Peak Resolution is the difference in retention times between the two peaks, divided by the combined widths of the elution peaks.
Retention Volume of Molecule 2 - (Measured in Cubic Meter) - Retention Volume of Molecule 2 is the volume that has passed through the column since the target molecule 2 was introduced onto the column.
Retention Volume of Molecule 1 - (Measured in Cubic Meter) - Retention Volume of Molecule 1 is the volume that has passed through the column since the target molecule 1 was introduced onto the column.
Width of Chromatographic Peak of Molecule 1 - (Measured in Meter) - Width of Chromatographic Peak of Molecule 1 is the distance between points of molecule 1 where lines tangent to the peak's left and right inflection points intersect the baseline.
Widths of Chromatographic Peak of Molecule 2 - (Measured in Meter) - Widths of Chromatographic Peak of Molecule 2 is the distance between points of molecule 2 where lines tangent to the peak's left and right inflection points intersect the baseline.

STEP 1: Convert Input(s) to Base Unit

Retention Volume of Molecule 2: 10.5 Milliliter --> 1.05E-05 Cubic Meter (Check conversion here)
Retention Volume of Molecule 1: 9.56 Milliliter --> 9.56E-06 Cubic Meter (Check conversion here)
Width of Chromatographic Peak of Molecule 1: 30 Millimeter --> 0.03 Meter (Check conversion here)
Widths of Chromatographic Peak of Molecule 2: 27 Millimeter --> 0.027 Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

R_s = (V_R2-V_R1)/((W_b1+W_b2)/2) --> (1.05E-05-9.56E-06)/((0.03+0.027)/2)

Evaluating ... ...

R_s = 3.29824561403509E-05

STEP 3: Convert Result to Output's Unit

3.29824561403509E-05 --> No Conversion Required

FINAL ANSWER

3.29824561403509E-05 ≈ 3.3E-5 <-- Peak Resolution

(Calculation completed in 00.004 seconds)

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Home » Chemistry » Biochemistry » Osmolality » Peak Resolution in Chromatography

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Created by Soupayan banerjee

National University of Judicial Science (NUJS), Kolkata

Soupayan banerjee has created this Calculator and 200+ more calculators!

Verified by Pratibha

Amity Institute Of Applied Sciences (AIAS, Amity University), Noida, India

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< 17 Osmolality Calculators

Peak Resolution in Chromatography

Go Peak Resolution = (Retention Volume of Molecule 2-Retention Volume of Molecule 1)/((Width of Chromatographic Peak of Molecule 1+Widths of Chromatographic Peak of Molecule 2)/2)

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Serum Glucose using Calculated Serum Osmolality

Go Serum Glucose = 18*((Calculated Serum Osmolality)-(2*Serum Sodium)-(Blood Urea Nitrogen/2.8))

Serum Sodium using Calculated Serum Osmolality

Go Serum Sodium = ((Calculated Serum Osmolality)-(Serum Glucose/18)-(Blood Urea Nitrogen/2.8))/2

Calculated Serum Osmolality

Go Calculated Serum Osmolality = (2*Serum Sodium)+(Serum Glucose/18)+(Blood Urea Nitrogen/2.8)

Width of Chromatographic Peak using Column Efficiency

Go Width of Chromatographic Peak = Retention Volume/(sqrt(Column Efficiency/16))

Retention Volume using Column Efficiency

Go Retention Volume = Width of Chromatographic Peak*(sqrt(Column Efficiency/16))

Column Efficiency in Chromatography

Go Column Efficiency = 16*((Retention Volume/ Width of Chromatographic Peak)^2)

Increase of Splicing Potential by Wild-type Sequence

Go Splicing Potential = log10(Weight Factor for Increase of Splicing Potential)

Calculated Serum Osmolality using Osmolar Gap

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Measured Osmolality using Osmolar Gap

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Go Splicing Potential = -log10(Weight Factor)

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Plasma Concentration using Plasma Osmolality

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Peak Resolution in Chromatography Formula

What is Column Chromatography?

Column chromatography separates substances based on differential adsorption of compounds to the adsorbent as the compounds move through the column at different rates which allow them to get separated in fractions. This technique can be used on a small scale as well as large scale to purify materials that can be used in future experiments. This method is a type of adsorption chromatography technique.

How to Calculate Peak Resolution in Chromatography?

Peak Resolution in Chromatography calculator uses Peak Resolution = (Retention Volume of Molecule 2-Retention Volume of Molecule 1)/((Width of Chromatographic Peak of Molecule 1+Widths of Chromatographic Peak of Molecule 2)/2) to calculate the Peak Resolution, The Peak Resolution in Chromatography formula is defined resolution is a measure of the separation of two peaks of different retention time in a chromatogram. Peak Resolution is denoted by R_s symbol.

How to calculate Peak Resolution in Chromatography using this online calculator? To use this online calculator for Peak Resolution in Chromatography, enter Retention Volume of Molecule 2 (V_R2), Retention Volume of Molecule 1 (V_R1), Width of Chromatographic Peak of Molecule 1 (W_b1) & Widths of Chromatographic Peak of Molecule 2 (W_b2) and hit the calculate button. Here is how the Peak Resolution in Chromatography calculation can be explained with given input values -> -0.000263 = (1.05E-05-9.56E-06)/((0.03+0.027)/2).

FAQ

What is Peak Resolution in Chromatography?

The Peak Resolution in Chromatography formula is defined resolution is a measure of the separation of two peaks of different retention time in a chromatogram and is represented as R_s = (V_R2-V_R1)/((W_b1+W_b2)/2) or Peak Resolution = (Retention Volume of Molecule 2-Retention Volume of Molecule 1)/((Width of Chromatographic Peak of Molecule 1+Widths of Chromatographic Peak of Molecule 2)/2). Retention Volume of Molecule 2 is the volume that has passed through the column since the target molecule 2 was introduced onto the column, Retention Volume of Molecule 1 is the volume that has passed through the column since the target molecule 1 was introduced onto the column, Width of Chromatographic Peak of Molecule 1 is the distance between points of molecule 1 where lines tangent to the peak's left and right inflection points intersect the baseline & Widths of Chromatographic Peak of Molecule 2 is the distance between points of molecule 2 where lines tangent to the peak's left and right inflection points intersect the baseline.

How to calculate Peak Resolution in Chromatography?

The Peak Resolution in Chromatography formula is defined resolution is a measure of the separation of two peaks of different retention time in a chromatogram is calculated using Peak Resolution = (Retention Volume of Molecule 2-Retention Volume of Molecule 1)/((Width of Chromatographic Peak of Molecule 1+Widths of Chromatographic Peak of Molecule 2)/2). To calculate Peak Resolution in Chromatography, you need Retention Volume of Molecule 2 (V_R2), Retention Volume of Molecule 1 (V_R1), Width of Chromatographic Peak of Molecule 1 (W_b1) & Widths of Chromatographic Peak of Molecule 2 (W_b2). With our tool, you need to enter the respective value for Retention Volume of Molecule 2, Retention Volume of Molecule 1, Width of Chromatographic Peak of Molecule 1 & Widths of Chromatographic Peak of Molecule 2 and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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