Quenching Concentration Calculator

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Physical spectroscopy

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Emission Spectroscopy

Absorption Spectroscopy

Raman spectroscopy

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Vibrational Spectroscopy

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Fluoroscence and Phosphorescence

Quantum Yield and Singlet Llifetime

✖Initial Intensity flux of radiant energy is the power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy.ⓘ Initial Intensity [Io]			+10% -10%
✖Fluorosence Intensity formula is defined as the power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy.ⓘ Fluorosence Intensity [I_F]			+10% -10%
✖Stern Volmner Constant is the bimolecular rate constant of the fluorescence quenching process due to a short-range interaction of species.ⓘ Stern Volmner Constant [K_SV]			+10% -10%

✖Quencher Concentration is the concentration of substance that decreases fluoroscence intensity.ⓘ Quenching Concentration [[Q1]]

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Formula

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Quenching Concentration

Formula

`"[Q1]" = (("Io"/"I"_{"F"})-1)/"K"_{"SV"}`

Example

`"216.6667"=(("500W/m²"/"240W/m²")-1)/"0.005"`

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Quenching Concentration Solution

STEP 0: Pre-Calculation Summary

Formula Used

Quencher Concentration = ((Initial Intensity/Fluorosence Intensity)-1)/Stern Volmner Constant
[Q1] = ((Io/I_F)-1)/K_SV
This formula uses 4 Variables

Variables Used

Quencher Concentration - Quencher Concentration is the concentration of substance that decreases fluoroscence intensity.
Initial Intensity - (Measured in Watt per Square Meter) - Initial Intensity flux of radiant energy is the power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy.
Fluorosence Intensity - (Measured in Watt per Square Meter) - Fluorosence Intensity formula is defined as the power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy.
Stern Volmner Constant - Stern Volmner Constant is the bimolecular rate constant of the fluorescence quenching process due to a short-range interaction of species.

STEP 1: Convert Input(s) to Base Unit

Initial Intensity: 500 Watt per Square Meter --> 500 Watt per Square Meter No Conversion Required
Fluorosence Intensity: 240 Watt per Square Meter --> 240 Watt per Square Meter No Conversion Required
Stern Volmner Constant: 0.005 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

[Q1] = ((Io/I_F)-1)/K_SV --> ((500/240)-1)/0.005

Evaluating ... ...

[Q1] = 216.666666666667

STEP 3: Convert Result to Output's Unit

216.666666666667 --> No Conversion Required

FINAL ANSWER

216.666666666667 ≈ 216.6667 <-- Quencher Concentration

(Calculation completed in 00.004 seconds)

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< 25 Emission Spectroscopy Calculators

Intensity of Fluorescence given Degree of Exciplex Formation

Go Fluorosence Intensity given Degree of Exciplex = Rate Constant of Fluoroscence*Equilibrium Constant for Coordinate Complexes*(1-Degree of Exciplex Formation)/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)

Degree of Exciplex Formation

Go Degree of Exciplex Formation = (Equilibrium Constant for Coordinate Complexes*Quencher Concentration given Degree of Exciplex)/(1+(Equilibrium Constant for Coordinate Complexes*Quencher Concentration given Degree of Exciplex))

Fluoroscence Quantum Yield given Phosphorescence Quantum Yield

Go Fluorosecence Quantum Yield given Ph = Phosphosecence Quantum Yield*((Rate Constant of Fluoroscence*Singlet State Concentration)/(Phosphorescence Rate Constant*Concentration of Triplet State))

Fluorosence Intensity at Low Concentration of Solute

Go Fluorosence Intensity at Low Concentration = Fluorosecence Quantum Yield*Initial Intensity*2.303*Spectroscopical Molar Extinction Coefficient*Concentration at Time t*Length

Fluorescence Quantum Yield

Go Quantum Yield of Fluorescence = Rate of Radiative Reaction/(Rate of Radiative Reaction+Rate of Internal Conversion+Rate Constant of Intersystem Crossing+Quenching Constant)

Initial Intensity given Degree of Exciplex Formation

Go Initial Intensity given Degree of Exciplex = Rate Constant of Fluoroscence*Equilibrium Constant for Coordinate Complexes/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)

Intensity Ratio

Go Intensity Ratio = 1+(Quencher Concentration given Degree of Exciplex*(Quenching Constant/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)))

Quantum Yield of Fluorescence

Go Quantum Yield of Fluorescence = Rate Constant of Fluoroscence/(Rate Constant of Fluoroscence+Rate of Internal Conversion+Rate Constant of Intersystem Crossing)

Singlet Life Time of Radiative Process

Go Singlet Life time of Radiative Process = ((Initial Intensity/Fluorosence Intensity)-1)/(Quenching Constant*Quencher Concentration given Degree of Exciplex)

Fluoroscence Intensity without Quenching

Go Intensity Without Quenching = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Non Radiative Reaction+Rate Constant of Fluoroscence)

Final Intensity using Stern Volmer Equation

Go Final Intensity = Initial Intensity/(1+(Singlet Life time given Degree of Exciplex*Quenching Constant*Quencher Concentration given Degree of Exciplex))

Fluoroscence Intensity

Go Fluorosence Intensity = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)

Singlet Life Time

Go Singlet Life time = 1/(Rate Constant of Intersystem Crossing+Rate of Radiative Reaction+Rate of Internal Conversion+Quenching Constant)

Collisional Energy Transfer

Go Rate of Collisional Energy Transfer = Quenching Constant*Quencher Concentration given Degree of Exciplex*Singlet State Concentration

Rate of Deactivation

Go Rate of Deactivation = (Rate Constant of Non Radiative Reaction+Rate Constant of Fluoroscence)*Singlet State Concentration

Quenching Concentration given Degree of Exciplex Formation

Go Quencher Concentration given Degree of Exciplex = ((1/(1-Degree of Exciplex Formation))-1)*(1/Equilibrium Constant for Coordinate Complexes)

Quenching Concentration

Go Quencher Concentration = ((Initial Intensity/Fluorosence Intensity)-1)/Stern Volmner Constant

Singlet Life given Degree of Exciplex Formation

Go Singlet Life time given Degree of Exciplex = 1/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)

Rate of Phosphorescence

Go Phosphorescence Rate = Phosphorescence Rate Constant*Concentration of Triplet State

Fluorescence Rate Constant

Go Rate Constant of Fluoroscence = Rate of Fluoroscence/Singlet State Concentration

Rate of Activation

Go Rate of Activation = Equilibrium Constant*(1-Degree of Dissociation of Emission)

ISC Rate Constant

Go Rate Constant of ISC = Rate of Intersystem Crossing*Singlet State Concentration

Difference in Acidity between Ground and Excited State

Go Difference in pka = pKa of Excited State-pKa of Ground State

Equilibrium Constant for Exciplex Formation

Go Equilibrium Constant for Coordinate Complexes = 1/(1-Degree of Exciplex Formation)-1

Singlet Radiative Phosphorescence Lifetime

Go Singlet Radiative Phosphorescence Lifetime = 1/Rate of Phosphorescence

< 12 Fluoroscence and Phosphorescence Calculators

Intensity of Fluorescence given Degree of Exciplex Formation

Singlet State Concentration

Go Concentration of Singlet State = Absorption Intensity/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction+Rate Constant of Intersystem Crossing+Rate Constant of Internal Conversion)

Initial Intensity given Degree of Exciplex Formation

Go Initial Intensity given Degree of Exciplex = Rate Constant of Fluoroscence*Equilibrium Constant for Coordinate Complexes/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)

Intensity Ratio

Go Intensity Ratio = 1+(Quencher Concentration given Degree of Exciplex*(Quenching Constant/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)))

Fluoroscence Intensity without Quenching

Go Intensity Without Quenching = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Non Radiative Reaction+Rate Constant of Fluoroscence)

Fluoroscence Intensity

Go Fluorosence Intensity = (Rate Constant of Fluoroscence*Absorption Intensity)/(Rate Constant of Fluoroscence+Rate Constant of Non Radiative Reaction)

Quenching Concentration

Go Quencher Concentration = ((Initial Intensity/Fluorosence Intensity)-1)/Stern Volmner Constant

Phosphorescence Rate Constant

Go Rate Constant of Phosphorescence = Rate of Phosphorescence/Concentration of Triplet State

Rate of Phosphorescence

Go Phosphorescence Rate = Phosphorescence Rate Constant*Concentration of Triplet State

Fluorescence Rate Constant

Go Rate Constant of Fluoroscence = Rate of Fluoroscence/Singlet State Concentration

ISC Rate Constant

Go Rate Constant of ISC = Rate of Intersystem Crossing*Singlet State Concentration

Singlet Radiative Fluorescence Lifetime

Go Singlet Radiative Fluorescence Lifetime = 1/Rate Constant of Fluoroscence

Quenching Concentration Formula

Quencher Concentration = ((Initial Intensity/Fluorosence Intensity)-1)/Stern Volmner Constant
[Q1] = ((Io/I_F)-1)/K_SV

What is concentration fluorescence quenching?

Quenching refers to any process which decreases the fluorescence intensity of a given substance. A variety of processes can result in quenching, such as excited state reactions, energy transfer, complex-formation and collisional quenching. As a consequence, quenching is often heavily dependent on pressure and temperature. Molecular oxygen, iodide ions and acrylamide[1] are common chemical quenchers. The chloride ion is a well known quencher for quinine fluorescence.[2][3][4] Quenching poses a problem for non-instant spectroscopic methods, such as laser-induced fluorescence.

Quenching is made use of in optode sensors; for instance the quenching effect of oxygen on certain ruthenium complexes allows the measurement of oxygen saturation in solution.

How to Calculate Quenching Concentration?

Quenching Concentration calculator uses Quencher Concentration = ((Initial Intensity/Fluorosence Intensity)-1)/Stern Volmner Constant to calculate the Quencher Concentration, The Quenching Concentration formula is defined as the concentration of an activator which is higher than an appropriate value (typically several mol%) for which the emission intensity of the phosphor mostly will be decreased. Quencher Concentration is denoted by [Q1] symbol.

How to calculate Quenching Concentration using this online calculator? To use this online calculator for Quenching Concentration, enter Initial Intensity (Io), Fluorosence Intensity (I_F) & Stern Volmner Constant (K_SV) and hit the calculate button. Here is how the Quenching Concentration calculation can be explained with given input values -> -216.666667 = ((500/240)-1)/0.005.

FAQ

What is Quenching Concentration?

The Quenching Concentration formula is defined as the concentration of an activator which is higher than an appropriate value (typically several mol%) for which the emission intensity of the phosphor mostly will be decreased and is represented as [Q1] = ((Io/I_F)-1)/K_SV or Quencher Concentration = ((Initial Intensity/Fluorosence Intensity)-1)/Stern Volmner Constant. Initial Intensity flux of radiant energy is the power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy, Fluorosence Intensity formula is defined as the power transferred per unit area, where the area is measured on the plane perpendicular to the direction of propagation of the energy & Stern Volmner Constant is the bimolecular rate constant of the fluorescence quenching process due to a short-range interaction of species.

How to calculate Quenching Concentration?

The Quenching Concentration formula is defined as the concentration of an activator which is higher than an appropriate value (typically several mol%) for which the emission intensity of the phosphor mostly will be decreased is calculated using Quencher Concentration = ((Initial Intensity/Fluorosence Intensity)-1)/Stern Volmner Constant. To calculate Quenching Concentration, you need Initial Intensity (Io), Fluorosence Intensity (I_F) & Stern Volmner Constant (K_SV). With our tool, you need to enter the respective value for Initial Intensity, Fluorosence Intensity & Stern Volmner Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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