Retention Factor Calculator

✖Solute Distance is the distance covered by a solute (stationary phase) on chromatography paper.ⓘ Solute Distance [d_solu]			+10% -10%
✖Solvent Distance is the distance covered by solvent (mobile phase) on chromatography paper.ⓘ Solvent Distance [d_solv]			+10% -10%

✖Actual Retention Factor is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front.ⓘ Retention Factor [RF]

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Formula

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Retention Factor

Formula

`"RF" = "d"_{"solu"}/"d"_{"solv"}`

Example

`"3.2"="80m"/"25m"`

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Download Method of Separation Technique Formula PDF

Retention Factor Solution

STEP 0: Pre-Calculation Summary

Formula Used

Actual Retention Factor = Solute Distance/Solvent Distance
RF = d_solu/d_solv
This formula uses 3 Variables

Variables Used

Actual Retention Factor - Actual Retention Factor is defined as the ratio of the distance traveled by the center of a spot to the distance traveled by the solvent front.
Solute Distance - (Measured in Meter) - Solute Distance is the distance covered by a solute (stationary phase) on chromatography paper.
Solvent Distance - (Measured in Meter) - Solvent Distance is the distance covered by solvent (mobile phase) on chromatography paper.

STEP 1: Convert Input(s) to Base Unit

Solute Distance: 80 Meter --> 80 Meter No Conversion Required
Solvent Distance: 25 Meter --> 25 Meter No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

RF = d_solu/d_solv --> 80/25

Evaluating ... ...

RF = 3.2

STEP 3: Convert Result to Output's Unit

3.2 --> No Conversion Required

FINAL ANSWER

3.2 <-- Actual Retention Factor

(Calculation completed in 00.004 seconds)

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Created by Pratibha

Amity Institute Of Applied Sciences (AIAS, Amity University), Noida, India

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Verified by Prerana Bakli

University of Hawaiʻi at Mānoa (UH Manoa), Hawaii, USA

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< 7 Retention Volume Calculators

Retention Volume given Capacity Factor

Go Retention Volume given CF = (Capacity Factor+1)*Unretained Mobile Phase Volume

Retention Volume given Flow Rate

Go Retention Volume given Flow Rate = (Retention Time*Flow Rate of Mobile Phase)

Half of Average Width of Peaks given Resolution and Change in Retention Volume

Go Half of Average Width of Peak = (0.589*Change in Retention Time)/Resolution

Average Width of Peak given Resolution and Change in Retention Volume

Go Average Width of Peaks given RV = (Change in retention volume/Resolution)

Flow Rate given Retention Volume and Time

Go Flow Rate given RT and RV = (Retention Volume/Retention Time)

Unretained Volume given Capacity Factor

Go Unretained Volume = (Retention Volume)/(Capacity Factor+1)

Retention Factor

Go Actual Retention Factor = Solute Distance/Solvent Distance

< 10+ Important Formulae on Retention and Deviation Calculators

Radius of First Column according to Scaling Equation

Go 1st Column Radius = (sqrt(Mass of 1st analyte/Mass of 2nd analyte))*Radius of 2nd column

Standard Deviation given Retention Time and Number of Theoretical Plates

Go Standard Deviation given RT and NP = (Retention Time)/(sqrt(Count of Theoretical Plates))

Mass of Second Analyte according to Scaling Equation

Go Mass of Analyte 2 = ((Radius of 2nd column/Radius of 1st column)^2)*Mass of 1st analyte

Width of Peak given Number of Theoretical Plates and Retention Time

Go Width of Peak NP and RT = (4*Retention Time)/(sqrt(Count of Theoretical Plates))

Retention Time given Capacity Factor

Go Retention Time given CF = Unretained Solute Travel Time*(Capacity Factor for Analytical+1)

Adjusted Retention Time given Retention Time

Go Adjusted Retention Time given RT = (Retention Time-Unretained Solute Travel Time)

Average Width of Peak given Resolution and Change in Retention Volume

Go Average Width of Peaks given RV = (Change in retention volume/Resolution)

Average Width of Peak given Resolution and Change in Retention Time

Go Average Width of Peaks given RT = (Change in Retention Time/Resolution)

Time for Diffusion given Standard Deviation

Go Diffusion Time = ((Standard Deviation)^2)/(2*Diffusion Coefficient)

Retention Factor

Go Actual Retention Factor = Solute Distance/Solvent Distance

Retention Factor Formula

Actual Retention Factor = Solute Distance/Solvent Distance
RF = d_solu/d_solv

What is Chromatography?

In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components. The mixture is dissolved in a fluid solvent (gas or liquid) called the mobile phase, which carries it through a system (a column, a capillary tube, a plate, or a sheet) on which a material called the stationary phase is fixed. Because the different constituents of the mixture tend to have different affinities for the stationary phase and are retained for different lengths of time depending on their interactions with its surface sites, the constituents travel at different apparent velocities in the mobile fluid, causing them to separate. The separation is based on the differential partitioning between the mobile and stationary phases. Subtle differences in a compound's partition coefficient result in differential retention on the stationary phase and thus affect the separation.

How to Calculate Retention Factor?

Retention Factor calculator uses Actual Retention Factor = Solute Distance/Solvent Distance to calculate the Actual Retention Factor, The Retention Factor formula is defined as the distance travelled by a given component divided by the distance travelled by the solvent front. Actual Retention Factor is denoted by RF symbol.

How to calculate Retention Factor using this online calculator? To use this online calculator for Retention Factor, enter Solute Distance (d_solu) & Solvent Distance (d_solv) and hit the calculate button. Here is how the Retention Factor calculation can be explained with given input values -> 3.2 = 80/25.

FAQ

What is Retention Factor?

The Retention Factor formula is defined as the distance travelled by a given component divided by the distance travelled by the solvent front and is represented as RF = d_solu/d_solv or Actual Retention Factor = Solute Distance/Solvent Distance. Solute Distance is the distance covered by a solute (stationary phase) on chromatography paper & Solvent Distance is the distance covered by solvent (mobile phase) on chromatography paper.

How to calculate Retention Factor?

The Retention Factor formula is defined as the distance travelled by a given component divided by the distance travelled by the solvent front is calculated using Actual Retention Factor = Solute Distance/Solvent Distance. To calculate Retention Factor, you need Solute Distance (d_solu) & Solvent Distance (d_solv). With our tool, you need to enter the respective value for Solute Distance & Solvent Distance and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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