Substrate Concentration in Enzymatic Reaction Mechanism Calculator

✖The Reverse Rate Constant is defined as the rate constant for the backward reaction.ⓘ Reverse Rate Constant [k_r]			+10% -10%
✖The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.ⓘ Enzyme Substrate Complex Concentration [ES]			+10% -10%
✖The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.ⓘ Forward Rate Constant [k_f]			+10% -10%
✖The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.ⓘ Initial Enzyme Concentration [[E₀]]			+10% -10%

✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration in Enzymatic Reaction Mechanism [S]

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Formula

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Substrate Concentration in Enzymatic Reaction Mechanism

Formula

`"S" = ("k"_{"r"}*"ES")/("k"_{"f"}*(("[E"_{"0"}"]")-"ES"))`

Example

`"0.322061mol/L"=("20mol/L*s"*"10mol/L")/("6.9s⁻¹"*("100mol/L"-"10mol/L"))`

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Substrate Concentration in Enzymatic Reaction Mechanism Solution

STEP 0: Pre-Calculation Summary

Formula Used

Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))
S = (k_r*ES)/(k_f*([E₀]-ES))
This formula uses 5 Variables

Variables Used

Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Reverse Rate Constant - (Measured in Mole per Cubic Meter Second) - The Reverse Rate Constant is defined as the rate constant for the backward reaction.
Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Forward Rate Constant - (Measured in 1 Per Second) - The Forward Rate Constant is defined as the rate constant for the forward occurring reaction.
Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.

STEP 1: Convert Input(s) to Base Unit

Reverse Rate Constant: 20 Mole per Liter Second --> 20000 Mole per Cubic Meter Second (Check conversion here)
Enzyme Substrate Complex Concentration: 10 Mole per Liter --> 10000 Mole per Cubic Meter (Check conversion here)
Forward Rate Constant: 6.9 1 Per Second --> 6.9 1 Per Second No Conversion Required
Initial Enzyme Concentration: 100 Mole per Liter --> 100000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

S = (k_r*ES)/(k_f*([E₀]-ES)) --> (20000*10000)/(6.9*(100000-10000))

Evaluating ... ...

S = 322.061191626409

STEP 3: Convert Result to Output's Unit

322.061191626409 Mole per Cubic Meter -->0.322061191626409 Mole per Liter (Check conversion here)

FINAL ANSWER

0.322061191626409 ≈ 0.322061 Mole per Liter <-- Substrate Concentration

(Calculation completed in 00.020 seconds)

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< 21 Complex Concentration Calculators

Enzyme Substrate Complex Concentration in Instantaneous Chemical Equilibrium

Go Enzyme Substrate Complex Concentration = (Forward Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/(Reverse Rate Constant+(Forward Rate Constant*Substrate Concentration))

Initial Enzyme Concentration in Enzymatic Reaction Mechanism

Go Initial Enzyme Concentration = ((Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration))+Enzyme Substrate Complex Concentration

Substrate Concentration in Enzymatic Reaction Mechanism

Go Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))

Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants

Go Catalyst Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration)

Substrate Concentration given Forward, Reverse, and Catalytic Rate Constants

Go Substrate Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Catalyst Concentration)

Initial Enzyme Concentration given Catalytic Rate Constant and Dissociation Rate Constants

Go Initial Enzyme Concentration = (Initial Reaction Rate*(Dissociation Rate Constant+Substrate Concentration))/(Catalytic Rate Constant*Substrate Concentration)

Substrate Concentration given Catalytic Rate Constant and Dissociation Rate Constants

Go Substrate Concentration = (Dissociation Rate Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Substrate Concentration given Catalytic Rate Constant and Initial Enzyme Concentration

Go Concentration of Substrate = (Michaelis Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Substrate Concentration given Dissociation Rate Constant

Go Substrate Concentration = (Dissociation Rate Constant*Enzyme Substrate Complex Concentration)/(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)

Initial Enzyme Concentration given Dissociation Rate Constant

Go Enzyme Concentration Initially = (Enzyme Substrate Complex Concentration*(Dissociation Rate Constant+Substrate Concentration))/(Substrate Concentration)

Enzyme Substrate Complex Concentration given Dissociation Rate Constant

Go Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)

Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration

Go Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration)

Initial Enzyme Concentration at Low Substrate Concentration

Go Initial Enzyme Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Substrate Concentration)

Inhibitor Concentration given Apparent Initial Enzyme Concentration

Go Inhibitor Concentration for CI = ((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1)*Enzyme Inhibitor Dissociation Constant

Substrate Concentration given Maximum Rate and Dissociation Rate Constant

Go Substrate Concentration = (Dissociation Rate Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)

Substrate Concentration given Maximum Rate at Low Concentration

Go Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/Maximum Rate

Inhibitor Concentration given Modifying Factor of Enzyme Substrate Complex

Go Inhibitor Concentration = (Enzyme Substrate Modifying Factor-1)*Enzyme Substrate Dissociation Constant

Inhibitor Concentration given Enzyme Substrate Modifying Factor

Go Inhibitor Concentration = (Enzyme Substrate Modifying Factor-1)*Enzyme Substrate Dissociation Constant

Inhibitor Concentration given Modifying Factor of Enzyme

Go Inhibitor Concentration = (Enzyme Modifying Factor-1)*Enzyme Inhibitor Dissociation Constant

Enzyme Substrate Complex Concentration given Rate Constant and Initial Rate

Go Enzyme Inhibitor Complex Concentration = (Initial Reaction Rate/Final Rate Constant)

Initial Enzyme Concentration given Rate Constant and Maximum Rate

Go Initial Enzyme Concentration = Maximum Rate/Final Rate Constant

Substrate Concentration in Enzymatic Reaction Mechanism Formula

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Substrate Concentration in Enzymatic Reaction Mechanism?

Substrate Concentration in Enzymatic Reaction Mechanism calculator uses Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)) to calculate the Substrate Concentration, The Substrate concentration in enzymatic reaction mechanism formula is defined as the relation with the rate constant of forward and backward reaction and with the concentration of enzyme and enzyme-substrate complex. Substrate Concentration is denoted by S symbol.

How to calculate Substrate Concentration in Enzymatic Reaction Mechanism using this online calculator? To use this online calculator for Substrate Concentration in Enzymatic Reaction Mechanism, enter Reverse Rate Constant (k_r), Enzyme Substrate Complex Concentration (ES), Forward Rate Constant (k_f) & Initial Enzyme Concentration ([E₀]) and hit the calculate button. Here is how the Substrate Concentration in Enzymatic Reaction Mechanism calculation can be explained with given input values -> 0.000322 = (20000*10000)/(6.9*(100000-10000)).

FAQ

What is Substrate Concentration in Enzymatic Reaction Mechanism?

The Substrate concentration in enzymatic reaction mechanism formula is defined as the relation with the rate constant of forward and backward reaction and with the concentration of enzyme and enzyme-substrate complex and is represented as S = (k_r*ES)/(k_f*([E₀]-ES)) or Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)). The Reverse Rate Constant is defined as the rate constant for the backward reaction, The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate, The Forward Rate Constant is defined as the rate constant for the forward occurring reaction & The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.

How to calculate Substrate Concentration in Enzymatic Reaction Mechanism?

The Substrate concentration in enzymatic reaction mechanism formula is defined as the relation with the rate constant of forward and backward reaction and with the concentration of enzyme and enzyme-substrate complex is calculated using Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)). To calculate Substrate Concentration in Enzymatic Reaction Mechanism, you need Reverse Rate Constant (k_r), Enzyme Substrate Complex Concentration (ES), Forward Rate Constant (k_f) & Initial Enzyme Concentration ([E₀]). With our tool, you need to enter the respective value for Reverse Rate Constant, Enzyme Substrate Complex Concentration, Forward Rate Constant & Initial Enzyme Concentration and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Substrate Concentration?

In this formula, Substrate Concentration uses Reverse Rate Constant, Enzyme Substrate Complex Concentration, Forward Rate Constant & Initial Enzyme Concentration. We can use 6 other way(s) to calculate the same, which is/are as follows -

Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration)
Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/Maximum Rate
Substrate Concentration = (Dissociation Rate Constant*Enzyme Substrate Complex Concentration)/(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)
Substrate Concentration = (Dissociation Rate Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)
Substrate Concentration = (Dissociation Rate Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)
Substrate Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Catalyst Concentration)

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