## Volume of Stationary Phase given Capacity Factor and Partition Coefficient Solution

STEP 0: Pre-Calculation Summary
Formula Used
Volume of Stationary Phase = (Capacity Factor*Volume of Mobile Phase)/Partition Coefficient
Vs = (k'*Vmobile phase)/K
This formula uses 4 Variables
Variables Used
Volume of Stationary Phase - (Measured in Cubic Meter) - The Volume of Stationary Phase is the amount of motionless part of the chromatography column.
Capacity Factor - The Capacity factor is directly proportional to the retention factor. The longer a component is retained by the column, the greater is the capacity factor.
Volume of Mobile Phase - (Measured in Cubic Meter) - The Volume of Mobile Phase is the amount of the solvent that runs through the chromatography column.
Partition Coefficient - The Partition Coefficient is defined as the ratio of the equilibrium concentrations of a dissolved substance in a two-phase system consisting of two largely immiscible solvents.
STEP 1: Convert Input(s) to Base Unit
Capacity Factor: 3 --> No Conversion Required
Volume of Mobile Phase: 5 Liter --> 0.005 Cubic Meter (Check conversion ​here)
Partition Coefficient: 40 --> No Conversion Required
STEP 2: Evaluate Formula
Substituting Input Values in Formula
Vs = (k'*Vmobile phase)/K --> (3*0.005)/40
Evaluating ... ...
Vs = 0.000375
STEP 3: Convert Result to Output's Unit
0.000375 Cubic Meter -->0.375 Liter (Check conversion ​here)
0.375 Liter <-- Volume of Stationary Phase
(Calculation completed in 00.004 seconds)
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## <Volume and Concentration of Mobile and Stationary Phase Calculators

Volume of Mobile Phase given Capacity Factor
​ Go Volume of Mobile Phase = (Volume of Stationary Phase*Concentration of Stationary Phase)/(Capacity Factor*Concentration of Mobile Phase)
Volume of Stationary Phase given Capacity Factor
​ Go Volume of Stationary Phase = (Volume of Mobile Phase*Concentration of Mobile Phase*Capacity Factor)/Concentration of Stationary Phase
Volume of Stationary Phase given Capacity Factor and Partition Coefficient
​ Go Volume of Stationary Phase = (Capacity Factor*Volume of Mobile Phase)/Partition Coefficient
Volume of Mobile Phase given Capacity Factor and Partition Coefficient
​ Go Volume of Mobile Phase = Partition Coefficient*(Volume of Stationary Phase/Capacity Factor)

## Volume of Stationary Phase given Capacity Factor and Partition Coefficient Formula

Volume of Stationary Phase = (Capacity Factor*Volume of Mobile Phase)/Partition Coefficient
Vs = (k'*Vmobile phase)/K

## What is Chromatography?

A separation process based on the various partitioning coefficients of different solutes between the two phases.
Involving the interaction of solute(s) and two phases
Mobile phase: A gas or liquid that moves through the column.
Stationary phase: A solid or liquid that remains in place.

## What are the types of Chromatography?

2) Ion-exchange chromatography
3) Partition chromatography
4) Molecular Size exclusion chromatography
5) Affinity chromatography

## How to Calculate Volume of Stationary Phase given Capacity Factor and Partition Coefficient?

Volume of Stationary Phase given Capacity Factor and Partition Coefficient calculator uses Volume of Stationary Phase = (Capacity Factor*Volume of Mobile Phase)/Partition Coefficient to calculate the Volume of Stationary Phase, The Volume of stationary phase given capacity factor and partition coefficient formula is defined as the ratio of the volume of mobile phase and capacity factor to the partition coefficient of the solutes. Volume of Stationary Phase is denoted by Vs symbol.

How to calculate Volume of Stationary Phase given Capacity Factor and Partition Coefficient using this online calculator? To use this online calculator for Volume of Stationary Phase given Capacity Factor and Partition Coefficient, enter Capacity Factor (k'), Volume of Mobile Phase (Vmobile phase) & Partition Coefficient (K) and hit the calculate button. Here is how the Volume of Stationary Phase given Capacity Factor and Partition Coefficient calculation can be explained with given input values -> 375 = (3*0.005)/40.

### FAQ

What is Volume of Stationary Phase given Capacity Factor and Partition Coefficient?
The Volume of stationary phase given capacity factor and partition coefficient formula is defined as the ratio of the volume of mobile phase and capacity factor to the partition coefficient of the solutes and is represented as Vs = (k'*Vmobile phase)/K or Volume of Stationary Phase = (Capacity Factor*Volume of Mobile Phase)/Partition Coefficient. The Capacity factor is directly proportional to the retention factor. The longer a component is retained by the column, the greater is the capacity factor, The Volume of Mobile Phase is the amount of the solvent that runs through the chromatography column & The Partition Coefficient is defined as the ratio of the equilibrium concentrations of a dissolved substance in a two-phase system consisting of two largely immiscible solvents.
How to calculate Volume of Stationary Phase given Capacity Factor and Partition Coefficient?
The Volume of stationary phase given capacity factor and partition coefficient formula is defined as the ratio of the volume of mobile phase and capacity factor to the partition coefficient of the solutes is calculated using Volume of Stationary Phase = (Capacity Factor*Volume of Mobile Phase)/Partition Coefficient. To calculate Volume of Stationary Phase given Capacity Factor and Partition Coefficient, you need Capacity Factor (k'), Volume of Mobile Phase (Vmobile phase) & Partition Coefficient (K). With our tool, you need to enter the respective value for Capacity Factor, Volume of Mobile Phase & Partition Coefficient and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
How many ways are there to calculate Volume of Stationary Phase?
In this formula, Volume of Stationary Phase uses Capacity Factor, Volume of Mobile Phase & Partition Coefficient. We can use 1 other way(s) to calculate the same, which is/are as follows -
• Volume of Stationary Phase = (Volume of Mobile Phase*Concentration of Mobile Phase*Capacity Factor)/Concentration of Stationary Phase
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