## Volume of Stationary Phase given Capacity Factor Solution

STEP 0: Pre-Calculation Summary
Formula Used
Volume of Stationary Phase = (Volume of Mobile Phase*Concentration of Mobile Phase*Capacity Factor)/Concentration of Stationary Phase
Vs = (Vmobile phase*Cm*k')/Cs
This formula uses 5 Variables
Variables Used
Volume of Stationary Phase - (Measured in Cubic Meter) - The Volume of Stationary Phase is the amount of motionless part of the chromatography column.
Volume of Mobile Phase - (Measured in Cubic Meter) - The Volume of Mobile Phase is the amount of the solvent that runs through the chromatography column.
Concentration of Mobile Phase - (Measured in Mole per Cubic Meter) - The Concentration of Mobile Phase is the application of a solution that runs through the column.
Capacity Factor - The Capacity factor is directly proportional to the retention factor. The longer a component is retained by the column, the greater is the capacity factor.
Concentration of Stationary Phase - (Measured in Mole per Cubic Meter) - The Concentration of stationary phase is the application of the motionless part of the chromatography column.
STEP 1: Convert Input(s) to Base Unit
Volume of Mobile Phase: 5 Liter --> 0.005 Cubic Meter (Check conversion ​here)
Concentration of Mobile Phase: 6 Mole per Liter --> 6000 Mole per Cubic Meter (Check conversion ​here)
Capacity Factor: 3 --> No Conversion Required
Concentration of Stationary Phase: 10 Mole per Liter --> 10000 Mole per Cubic Meter (Check conversion ​here)
STEP 2: Evaluate Formula
Substituting Input Values in Formula
Vs = (Vmobile phase*Cm*k')/Cs --> (0.005*6000*3)/10000
Evaluating ... ...
Vs = 0.009
STEP 3: Convert Result to Output's Unit
0.009 Cubic Meter -->9 Liter (Check conversion ​here)
9 Liter <-- Volume of Stationary Phase
(Calculation completed in 00.004 seconds)
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K J Somaiya College of science (K J Somaiya), Mumbai
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## <Volume and Concentration of Mobile and Stationary Phase Calculators

Volume of Mobile Phase given Capacity Factor
​ Go Volume of Mobile Phase = (Volume of Stationary Phase*Concentration of Stationary Phase)/(Capacity Factor*Concentration of Mobile Phase)
Volume of Stationary Phase given Capacity Factor
​ Go Volume of Stationary Phase = (Volume of Mobile Phase*Concentration of Mobile Phase*Capacity Factor)/Concentration of Stationary Phase
Volume of Stationary Phase given Capacity Factor and Partition Coefficient
​ Go Volume of Stationary Phase = (Capacity Factor*Volume of Mobile Phase)/Partition Coefficient
Volume of Mobile Phase given Capacity Factor and Partition Coefficient
​ Go Volume of Mobile Phase = Partition Coefficient*(Volume of Stationary Phase/Capacity Factor)

## Volume of Stationary Phase given Capacity Factor Formula

Volume of Stationary Phase = (Volume of Mobile Phase*Concentration of Mobile Phase*Capacity Factor)/Concentration of Stationary Phase
Vs = (Vmobile phase*Cm*k')/Cs

## What is Chromatography?

A separation process based on the various partitioning coefficients of different solutes between the two phases.
Involving the interaction of solute(s) and two phases
Mobile phase: A gas or liquid that moves through the column.
Stationary phase: A solid or liquid that remains in place.

## What are the types of Chromatography?

2) Ion-exchange chromatography
3) Partition chromatography
4) Molecular Size exclusion chromatography
5) Affinity chromatography

## How to Calculate Volume of Stationary Phase given Capacity Factor?

Volume of Stationary Phase given Capacity Factor calculator uses Volume of Stationary Phase = (Volume of Mobile Phase*Concentration of Mobile Phase*Capacity Factor)/Concentration of Stationary Phase to calculate the Volume of Stationary Phase, The Volume of stationary phase given capacity factor formula is defined as the ratio of concentration and volume of mobile phase and capacity factor to the concentration of the stationary phase. Volume of Stationary Phase is denoted by Vs symbol.

How to calculate Volume of Stationary Phase given Capacity Factor using this online calculator? To use this online calculator for Volume of Stationary Phase given Capacity Factor, enter Volume of Mobile Phase (Vmobile phase), Concentration of Mobile Phase (Cm), Capacity Factor (k') & Concentration of Stationary Phase (Cs) and hit the calculate button. Here is how the Volume of Stationary Phase given Capacity Factor calculation can be explained with given input values -> 9000 = (0.005*6000*3)/10000.

### FAQ

What is Volume of Stationary Phase given Capacity Factor?
The Volume of stationary phase given capacity factor formula is defined as the ratio of concentration and volume of mobile phase and capacity factor to the concentration of the stationary phase and is represented as Vs = (Vmobile phase*Cm*k')/Cs or Volume of Stationary Phase = (Volume of Mobile Phase*Concentration of Mobile Phase*Capacity Factor)/Concentration of Stationary Phase. The Volume of Mobile Phase is the amount of the solvent that runs through the chromatography column, The Concentration of Mobile Phase is the application of a solution that runs through the column, The Capacity factor is directly proportional to the retention factor. The longer a component is retained by the column, the greater is the capacity factor & The Concentration of stationary phase is the application of the motionless part of the chromatography column.
How to calculate Volume of Stationary Phase given Capacity Factor?
The Volume of stationary phase given capacity factor formula is defined as the ratio of concentration and volume of mobile phase and capacity factor to the concentration of the stationary phase is calculated using Volume of Stationary Phase = (Volume of Mobile Phase*Concentration of Mobile Phase*Capacity Factor)/Concentration of Stationary Phase. To calculate Volume of Stationary Phase given Capacity Factor, you need Volume of Mobile Phase (Vmobile phase), Concentration of Mobile Phase (Cm), Capacity Factor (k') & Concentration of Stationary Phase (Cs). With our tool, you need to enter the respective value for Volume of Mobile Phase, Concentration of Mobile Phase, Capacity Factor & Concentration of Stationary Phase and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.
How many ways are there to calculate Volume of Stationary Phase?
In this formula, Volume of Stationary Phase uses Volume of Mobile Phase, Concentration of Mobile Phase, Capacity Factor & Concentration of Stationary Phase. We can use 1 other way(s) to calculate the same, which is/are as follows -
• Volume of Stationary Phase = (Capacity Factor*Volume of Mobile Phase)/Partition Coefficient
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