Width of Chromatographic Peak using Column Efficiency Calculator

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✖The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column.ⓘ Retention Volume [V_R]			+10% -10%
✖Column Efficiency, also known as plate count, is a measure of the dispersion of a peak.ⓘ Column Efficiency [N]			+10% -10%

✖Width of Chromatographic Peak is the distance between points where lines tangent to the peak's left and right inflection points intersect the baseline.ⓘ Width of Chromatographic Peak using Column Efficiency [W_b]

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Formula

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Width of Chromatographic Peak using Column Efficiency

Formula

`"W"_{"b"} = "V"_{"R"}/(sqrt("N"/16))`

Example

`"19.10278mm"="11.2L"/(sqrt("5.5"/16))`

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Width of Chromatographic Peak using Column Efficiency Solution

STEP 0: Pre-Calculation Summary

Formula Used

Width of Chromatographic Peak = Retention Volume/(sqrt(Column Efficiency/16))
W_b = V_R/(sqrt(N/16))
This formula uses 1 Functions, 3 Variables

Functions Used

sqrt - A square root function is a function that takes a non-negative number as an input and returns the square root of the given input number., sqrt(Number)

Variables Used

Width of Chromatographic Peak - (Measured in Meter) - Width of Chromatographic Peak is the distance between points where lines tangent to the peak's left and right inflection points intersect the baseline.
Retention Volume - (Measured in Cubic Meter) - The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column.
Column Efficiency - Column Efficiency, also known as plate count, is a measure of the dispersion of a peak.

STEP 1: Convert Input(s) to Base Unit

Retention Volume: 11.2 Liter --> 0.0112 Cubic Meter (Check conversion here)
Column Efficiency: 5.5 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

W_b = V_R/(sqrt(N/16)) --> 0.0112/(sqrt(5.5/16))

Evaluating ... ...

W_b = 0.0191027841854627

STEP 3: Convert Result to Output's Unit

0.0191027841854627 Meter -->19.1027841854627 Millimeter (Check conversion here)

FINAL ANSWER

19.1027841854627 ≈ 19.10278 Millimeter <-- Width of Chromatographic Peak

(Calculation completed in 00.020 seconds)

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National University of Judicial Science (NUJS), Kolkata

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Width of Chromatographic Peak using Column Efficiency Formula

Width of Chromatographic Peak = Retention Volume/(sqrt(Column Efficiency/16))
W_b = V_R/(sqrt(N/16))

What is Column Chromatography?

Column chromatography in chemistry is a chromatography method used to isolate a single chemical compound from a mixture. Chromatography is able to separate substances based on differential adsorption of compounds to the adsorbent; compounds move through the column at different rates, allowing them to be separated into fractions. The technique is widely applicable, as many different adsorbents (normal phase, reversed phase, or otherwise) can be used with a wide range of solvents.

How to Calculate Width of Chromatographic Peak using Column Efficiency?

Width of Chromatographic Peak using Column Efficiency calculator uses Width of Chromatographic Peak = Retention Volume/(sqrt(Column Efficiency/16)) to calculate the Width of Chromatographic Peak, The Width of Chromatographic Peak using Column Efficiency formula is defined as the peak's full width at half maximum. Lower peak widths indicates better chromatographic resolution. Width of Chromatographic Peak is denoted by W_b symbol.

How to calculate Width of Chromatographic Peak using Column Efficiency using this online calculator? To use this online calculator for Width of Chromatographic Peak using Column Efficiency, enter Retention Volume (V_R) & Column Efficiency (N) and hit the calculate button. Here is how the Width of Chromatographic Peak using Column Efficiency calculation can be explained with given input values -> 19102.78 = 0.0112/(sqrt(5.5/16)).

FAQ

What is Width of Chromatographic Peak using Column Efficiency?

The Width of Chromatographic Peak using Column Efficiency formula is defined as the peak's full width at half maximum. Lower peak widths indicates better chromatographic resolution and is represented as W_b = V_R/(sqrt(N/16)) or Width of Chromatographic Peak = Retention Volume/(sqrt(Column Efficiency/16)). The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column & Column Efficiency, also known as plate count, is a measure of the dispersion of a peak.

How to calculate Width of Chromatographic Peak using Column Efficiency?

The Width of Chromatographic Peak using Column Efficiency formula is defined as the peak's full width at half maximum. Lower peak widths indicates better chromatographic resolution is calculated using Width of Chromatographic Peak = Retention Volume/(sqrt(Column Efficiency/16)). To calculate Width of Chromatographic Peak using Column Efficiency, you need Retention Volume (V_R) & Column Efficiency (N). With our tool, you need to enter the respective value for Retention Volume & Column Efficiency and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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