Donor Lifetime using Rates of Transitions Calculator

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Förster resonance energy transfer

✖The Rate of Radiative Transitions is the radiative decay rate of the donor.ⓘ Rate of Radiative Transitions [K_r]			+10% -10%
✖The Rate of Non radiative Transitions is the rates of any other de-excitation pathways excluding energy transfers to other acceptors.ⓘ Rate of Non radiative Transitions [K_nr]			+10% -10%

✖The Donor Lifetime is the donor's fluorescence lifetime in the absence of the acceptor.ⓘ Donor Lifetime using Rates of Transitions [ζ_D]

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Formula

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Donor Lifetime using Rates of Transitions

Formula

`"ζ"_{"D"} = 1/("K"_{"r"}+"K"_{"nr"})`

Example

`"20"=1/("0.02"+"0.03")`

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Donor Lifetime using Rates of Transitions Solution

STEP 0: Pre-Calculation Summary

Formula Used

Donor Lifetime = 1/(Rate of Radiative Transitions+Rate of Non radiative Transitions)
ζ_D = 1/(K_r+K_nr)
This formula uses 3 Variables

Variables Used

Donor Lifetime - The Donor Lifetime is the donor's fluorescence lifetime in the absence of the acceptor.
Rate of Radiative Transitions - The Rate of Radiative Transitions is the radiative decay rate of the donor.
Rate of Non radiative Transitions - The Rate of Non radiative Transitions is the rates of any other de-excitation pathways excluding energy transfers to other acceptors.

STEP 1: Convert Input(s) to Base Unit

Rate of Radiative Transitions: 0.02 --> No Conversion Required
Rate of Non radiative Transitions: 0.03 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

ζ_D = 1/(K_r+K_nr) --> 1/(0.02+0.03)

Evaluating ... ...

ζ_D = 20

STEP 3: Convert Result to Output's Unit

20 --> No Conversion Required

FINAL ANSWER

20 <-- Donor Lifetime

(Calculation completed in 00.004 seconds)

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< 11 Förster resonance energy transfer Calculators

Forster Critical Distance

Go Forster Critical Distance = 0.0211*((Refractive Index of Medium)^(-4)*(Fluorescence Quantum Yield without FRET)*(Orientation Factor)*(Spectral Overlap Integral))^(1/6)

Efficiency of Energy Transfer using Rate of Energy Transfer

Go Efficiency of Energy Transfer = Rate of Energy Transfer/(Rate of Energy Transfer+Rate of Non radiative Transitions+Rate of Radiative Transitions)

Donor Lifetime with FRET using Rate of Energy and Transitions

Go Donor Lifetime with FRET = 1/(Rate of Energy Transfer+Rate of Radiative Transitions+Rate of Non radiative Transitions)

Rate of Energy Transfer using Distances and Donor Lifetime

Go Rate of Energy Transfer = (1/Donor Lifetime)*(Forster Critical Distance/Donor to Acceptor Distance)^6

Efficiency of Energy Transfer using Photobleaching Decay Time Constant

Go Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET)

Efficiency of Energy Transfer using Distances

Go Efficiency of Energy Transfer = 1/(1+(Donor to Acceptor Distance/Forster Critical Distance)^6)

Efficiency of Energy Transfer using Fluorescence Intensity of Donor

Go Efficiency of Energy Transfer = 1-(Fluorescence Intensity with FRET/Fluorescence intensity)

Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime

Go Efficiency of Energy Transfer = Rate of Energy Transfer/(1/Donor Lifetime with FRET)

Donor Lifetime using Rates of Transitions

Go Donor Lifetime = 1/(Rate of Radiative Transitions+Rate of Non radiative Transitions)

Fluorescence Quantum Yield in FRET

Go Fluorescence Quantum Yield = Number of Photons Emitted/Number of Photons Absorbed

Efficiency of Energy Transfer using Donor Lifetime

Go Efficiency of Energy Transfer = 1-(Donor Lifetime with FRET/Donor Lifetime)

Donor Lifetime using Rates of Transitions Formula

Donor Lifetime = 1/(Rate of Radiative Transitions+Rate of Non radiative Transitions)
ζ_D = 1/(K_r+K_nr)

What is Fluorescence?

Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence.

How to Calculate Donor Lifetime using Rates of Transitions?

Donor Lifetime using Rates of Transitions calculator uses Donor Lifetime = 1/(Rate of Radiative Transitions+Rate of Non radiative Transitions) to calculate the Donor Lifetime, The Donor Lifetime using Rates of Transitions formula is defined as the inverse of the sum of rate of radiative transitions and rate of non radiative transitions. Donor Lifetime is denoted by ζ_D symbol.

How to calculate Donor Lifetime using Rates of Transitions using this online calculator? To use this online calculator for Donor Lifetime using Rates of Transitions, enter Rate of Radiative Transitions (K_r) & Rate of Non radiative Transitions (K_nr) and hit the calculate button. Here is how the Donor Lifetime using Rates of Transitions calculation can be explained with given input values -> 20 = 1/(0.02+0.03).

FAQ

What is Donor Lifetime using Rates of Transitions?

The Donor Lifetime using Rates of Transitions formula is defined as the inverse of the sum of rate of radiative transitions and rate of non radiative transitions and is represented as ζ_D = 1/(K_r+K_nr) or Donor Lifetime = 1/(Rate of Radiative Transitions+Rate of Non radiative Transitions). The Rate of Radiative Transitions is the radiative decay rate of the donor & The Rate of Non radiative Transitions is the rates of any other de-excitation pathways excluding energy transfers to other acceptors.

How to calculate Donor Lifetime using Rates of Transitions?

The Donor Lifetime using Rates of Transitions formula is defined as the inverse of the sum of rate of radiative transitions and rate of non radiative transitions is calculated using Donor Lifetime = 1/(Rate of Radiative Transitions+Rate of Non radiative Transitions). To calculate Donor Lifetime using Rates of Transitions, you need Rate of Radiative Transitions (K_r) & Rate of Non radiative Transitions (K_nr). With our tool, you need to enter the respective value for Rate of Radiative Transitions & Rate of Non radiative Transitions and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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