Donor Lifetime with FRET using Rate of Energy and Transitions Calculator

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Förster resonance energy transfer

✖Rate of Energy Transfer is simply the rate of energy transfer from a donor to an acceptor.ⓘ Rate of Energy Transfer [K_T]			+10% -10%
✖The Rate of Radiative Transitions is the radiative decay rate of the donor.ⓘ Rate of Radiative Transitions [K_r]			+10% -10%
✖The Rate of Non radiative Transitions is the rates of any other de-excitation pathways excluding energy transfers to other acceptors.ⓘ Rate of Non radiative Transitions [K_nr]			+10% -10%

✖The Donor Lifetime with FRET is the donor fluorescence lifetimes in the presence of an acceptor.ⓘ Donor Lifetime with FRET using Rate of Energy and Transitions [ζ_DA]

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Formula

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Donor Lifetime with FRET using Rate of Energy and Transitions

Formula

`"ζ"_{"DA"} = 1/("K"_{"T"}+"K"_{"r"}+"K"_{"nr"})`

Example

`"16.66667"=1/("0.01"+"0.02"+"0.03")`

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Donor Lifetime with FRET using Rate of Energy and Transitions Solution

STEP 0: Pre-Calculation Summary

Formula Used

Donor Lifetime with FRET = 1/(Rate of Energy Transfer+Rate of Radiative Transitions+Rate of Non radiative Transitions)
ζ_DA = 1/(K_T+K_r+K_nr)
This formula uses 4 Variables

Variables Used

Donor Lifetime with FRET - The Donor Lifetime with FRET is the donor fluorescence lifetimes in the presence of an acceptor.
Rate of Energy Transfer - Rate of Energy Transfer is simply the rate of energy transfer from a donor to an acceptor.
Rate of Radiative Transitions - The Rate of Radiative Transitions is the radiative decay rate of the donor.
Rate of Non radiative Transitions - The Rate of Non radiative Transitions is the rates of any other de-excitation pathways excluding energy transfers to other acceptors.

STEP 1: Convert Input(s) to Base Unit

Rate of Energy Transfer: 0.01 --> No Conversion Required
Rate of Radiative Transitions: 0.02 --> No Conversion Required
Rate of Non radiative Transitions: 0.03 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

ζ_DA = 1/(K_T+K_r+K_nr) --> 1/(0.01+0.02+0.03)

Evaluating ... ...

ζ_DA = 16.6666666666667

STEP 3: Convert Result to Output's Unit

16.6666666666667 --> No Conversion Required

FINAL ANSWER

16.6666666666667 ≈ 16.66667 <-- Donor Lifetime with FRET

(Calculation completed in 00.004 seconds)

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Home » Chemistry » Physical Chemistry » Physical spectroscopy » Emission Spectroscopy » Fluoroscence and Phosphorescence » Förster resonance energy transfer » Donor Lifetime with FRET using Rate of Energy and Transitions

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< 11 Förster resonance energy transfer Calculators

Forster Critical Distance

Go Forster Critical Distance = 0.0211*((Refractive Index of Medium)^(-4)*(Fluorescence Quantum Yield without FRET)*(Orientation Factor)*(Spectral Overlap Integral))^(1/6)

Efficiency of Energy Transfer using Rate of Energy Transfer

Go Efficiency of Energy Transfer = Rate of Energy Transfer/(Rate of Energy Transfer+Rate of Non radiative Transitions+Rate of Radiative Transitions)

Donor Lifetime with FRET using Rate of Energy and Transitions

Go Donor Lifetime with FRET = 1/(Rate of Energy Transfer+Rate of Radiative Transitions+Rate of Non radiative Transitions)

Rate of Energy Transfer using Distances and Donor Lifetime

Go Rate of Energy Transfer = (1/Donor Lifetime)*(Forster Critical Distance/Donor to Acceptor Distance)^6

Efficiency of Energy Transfer using Photobleaching Decay Time Constant

Go Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET)

Efficiency of Energy Transfer using Distances

Go Efficiency of Energy Transfer = 1/(1+(Donor to Acceptor Distance/Forster Critical Distance)^6)

Efficiency of Energy Transfer using Fluorescence Intensity of Donor

Go Efficiency of Energy Transfer = 1-(Fluorescence Intensity with FRET/Fluorescence intensity)

Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime

Go Efficiency of Energy Transfer = Rate of Energy Transfer/(1/Donor Lifetime with FRET)

Donor Lifetime using Rates of Transitions

Go Donor Lifetime = 1/(Rate of Radiative Transitions+Rate of Non radiative Transitions)

Fluorescence Quantum Yield in FRET

Go Fluorescence Quantum Yield = Number of Photons Emitted/Number of Photons Absorbed

Efficiency of Energy Transfer using Donor Lifetime

Go Efficiency of Energy Transfer = 1-(Donor Lifetime with FRET/Donor Lifetime)

Donor Lifetime with FRET using Rate of Energy and Transitions Formula

Donor Lifetime with FRET = 1/(Rate of Energy Transfer+Rate of Radiative Transitions+Rate of Non radiative Transitions)
ζ_DA = 1/(K_T+K_r+K_nr)

What is Phosphorescence?

Phosphorescence is a type of photoluminescence related to fluorescence. When exposed to light (radiation) of a shorter wavelength, a phosphorescent substance will glow, absorbing the light and reemitting it at a longer wavelength.

How to Calculate Donor Lifetime with FRET using Rate of Energy and Transitions?

Donor Lifetime with FRET using Rate of Energy and Transitions calculator uses Donor Lifetime with FRET = 1/(Rate of Energy Transfer+Rate of Radiative Transitions+Rate of Non radiative Transitions) to calculate the Donor Lifetime with FRET, The Donor Lifetime with FRET using Rate of Energy and Transitions formula is defined as the donor fluorescence lifetimes in the presence of an acceptor which can be calculated as the inverse of the sum of rate of energy transfer, rate of radiative transitions and rate of non-radiative transitions. Donor Lifetime with FRET is denoted by ζ_DA symbol.

How to calculate Donor Lifetime with FRET using Rate of Energy and Transitions using this online calculator? To use this online calculator for Donor Lifetime with FRET using Rate of Energy and Transitions, enter Rate of Energy Transfer (K_T), Rate of Radiative Transitions (K_r) & Rate of Non radiative Transitions (K_nr) and hit the calculate button. Here is how the Donor Lifetime with FRET using Rate of Energy and Transitions calculation can be explained with given input values -> 16.66667 = 1/(0.01+0.02+0.03).

FAQ

What is Donor Lifetime with FRET using Rate of Energy and Transitions?

The Donor Lifetime with FRET using Rate of Energy and Transitions formula is defined as the donor fluorescence lifetimes in the presence of an acceptor which can be calculated as the inverse of the sum of rate of energy transfer, rate of radiative transitions and rate of non-radiative transitions and is represented as ζ_DA = 1/(K_T+K_r+K_nr) or Donor Lifetime with FRET = 1/(Rate of Energy Transfer+Rate of Radiative Transitions+Rate of Non radiative Transitions). Rate of Energy Transfer is simply the rate of energy transfer from a donor to an acceptor, The Rate of Radiative Transitions is the radiative decay rate of the donor & The Rate of Non radiative Transitions is the rates of any other de-excitation pathways excluding energy transfers to other acceptors.

How to calculate Donor Lifetime with FRET using Rate of Energy and Transitions?

The Donor Lifetime with FRET using Rate of Energy and Transitions formula is defined as the donor fluorescence lifetimes in the presence of an acceptor which can be calculated as the inverse of the sum of rate of energy transfer, rate of radiative transitions and rate of non-radiative transitions is calculated using Donor Lifetime with FRET = 1/(Rate of Energy Transfer+Rate of Radiative Transitions+Rate of Non radiative Transitions). To calculate Donor Lifetime with FRET using Rate of Energy and Transitions, you need Rate of Energy Transfer (K_T), Rate of Radiative Transitions (K_r) & Rate of Non radiative Transitions (K_nr). With our tool, you need to enter the respective value for Rate of Energy Transfer, Rate of Radiative Transitions & Rate of Non radiative Transitions and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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