Efficiency of Energy Transfer using Donor Lifetime Calculator

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Physical spectroscopy

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Emission Spectroscopy

Absorption Spectroscopy

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Vibrational Spectroscopy

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Fluoroscence and Phosphorescence

Quantum Yield and Singlet Llifetime

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Förster resonance energy transfer

✖The Donor Lifetime with FRET is the donor fluorescence lifetimes in the presence of an acceptor.ⓘ Donor Lifetime with FRET [ζ_DA]			+10% -10%
✖The Donor Lifetime is the donor's fluorescence lifetime in the absence of the acceptor.ⓘ Donor Lifetime [ζ_D]			+10% -10%

✖The Efficiency of Energy Transfer describes the efficiency of energy transfer between two light-sensitive molecules (chromophores).ⓘ Efficiency of Energy Transfer using Donor Lifetime [E]

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Formula

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Efficiency of Energy Transfer using Donor Lifetime

Formula

`"E" = 1-("ζ"_{"DA"}/"ζ"_{"D"})`

Example

`"0.6"=1-("0.004"/"0.01")`

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Efficiency of Energy Transfer using Donor Lifetime Solution

STEP 0: Pre-Calculation Summary

Formula Used

Efficiency of Energy Transfer = 1-(Donor Lifetime with FRET/Donor Lifetime)
E = 1-(ζ_DA/ζ_D)
This formula uses 3 Variables

Variables Used

Efficiency of Energy Transfer - The Efficiency of Energy Transfer describes the efficiency of energy transfer between two light-sensitive molecules (chromophores).
Donor Lifetime with FRET - The Donor Lifetime with FRET is the donor fluorescence lifetimes in the presence of an acceptor.
Donor Lifetime - The Donor Lifetime is the donor's fluorescence lifetime in the absence of the acceptor.

STEP 1: Convert Input(s) to Base Unit

Donor Lifetime with FRET: 0.004 --> No Conversion Required
Donor Lifetime: 0.01 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

E = 1-(ζ_DA/ζ_D) --> 1-(0.004/0.01)

Evaluating ... ...

E = 0.6

STEP 3: Convert Result to Output's Unit

0.6 --> No Conversion Required

FINAL ANSWER

0.6 <-- Efficiency of Energy Transfer

(Calculation completed in 00.004 seconds)

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< 11 Förster resonance energy transfer Calculators

Forster Critical Distance

Go Forster Critical Distance = 0.0211*((Refractive Index of Medium)^(-4)*(Fluorescence Quantum Yield without FRET)*(Orientation Factor)*(Spectral Overlap Integral))^(1/6)

Efficiency of Energy Transfer using Rate of Energy Transfer

Go Efficiency of Energy Transfer = Rate of Energy Transfer/(Rate of Energy Transfer+Rate of Non radiative Transitions+Rate of Radiative Transitions)

Donor Lifetime with FRET using Rate of Energy and Transitions

Go Donor Lifetime with FRET = 1/(Rate of Energy Transfer+Rate of Radiative Transitions+Rate of Non radiative Transitions)

Rate of Energy Transfer using Distances and Donor Lifetime

Go Rate of Energy Transfer = (1/Donor Lifetime)*(Forster Critical Distance/Donor to Acceptor Distance)^6

Efficiency of Energy Transfer using Photobleaching Decay Time Constant

Go Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET)

Efficiency of Energy Transfer using Distances

Go Efficiency of Energy Transfer = 1/(1+(Donor to Acceptor Distance/Forster Critical Distance)^6)

Efficiency of Energy Transfer using Fluorescence Intensity of Donor

Go Efficiency of Energy Transfer = 1-(Fluorescence Intensity with FRET/Fluorescence intensity)

Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime

Go Efficiency of Energy Transfer = Rate of Energy Transfer/(1/Donor Lifetime with FRET)

Donor Lifetime using Rates of Transitions

Go Donor Lifetime = 1/(Rate of Radiative Transitions+Rate of Non radiative Transitions)

Fluorescence Quantum Yield in FRET

Go Fluorescence Quantum Yield = Number of Photons Emitted/Number of Photons Absorbed

Efficiency of Energy Transfer using Donor Lifetime

Go Efficiency of Energy Transfer = 1-(Donor Lifetime with FRET/Donor Lifetime)

Efficiency of Energy Transfer using Donor Lifetime Formula

Efficiency of Energy Transfer = 1-(Donor Lifetime with FRET/Donor Lifetime)
E = 1-(ζ_DA/ζ_D)

What are chromophores?

Chromophores are regions in a molecule where the energy difference between two molecular orbitals falls within the visible spectrum.

How to Calculate Efficiency of Energy Transfer using Donor Lifetime?

Efficiency of Energy Transfer using Donor Lifetime calculator uses Efficiency of Energy Transfer = 1-(Donor Lifetime with FRET/Donor Lifetime) to calculate the Efficiency of Energy Transfer, The Efficiency of Energy Transfer using Donor Lifetime formula is defined as the ratio of diffrence of donor lifetime without and with FRET to donor lifetime without FRET. Efficiency of Energy Transfer is denoted by E symbol.

How to calculate Efficiency of Energy Transfer using Donor Lifetime using this online calculator? To use this online calculator for Efficiency of Energy Transfer using Donor Lifetime, enter Donor Lifetime with FRET (ζ_DA) & Donor Lifetime (ζ_D) and hit the calculate button. Here is how the Efficiency of Energy Transfer using Donor Lifetime calculation can be explained with given input values -> -39 = 1-(0.004/0.01).

FAQ

What is Efficiency of Energy Transfer using Donor Lifetime?

The Efficiency of Energy Transfer using Donor Lifetime formula is defined as the ratio of diffrence of donor lifetime without and with FRET to donor lifetime without FRET and is represented as E = 1-(ζ_DA/ζ_D) or Efficiency of Energy Transfer = 1-(Donor Lifetime with FRET/Donor Lifetime). The Donor Lifetime with FRET is the donor fluorescence lifetimes in the presence of an acceptor & The Donor Lifetime is the donor's fluorescence lifetime in the absence of the acceptor.

How to calculate Efficiency of Energy Transfer using Donor Lifetime?

The Efficiency of Energy Transfer using Donor Lifetime formula is defined as the ratio of diffrence of donor lifetime without and with FRET to donor lifetime without FRET is calculated using Efficiency of Energy Transfer = 1-(Donor Lifetime with FRET/Donor Lifetime). To calculate Efficiency of Energy Transfer using Donor Lifetime, you need Donor Lifetime with FRET (ζ_DA) & Donor Lifetime (ζ_D). With our tool, you need to enter the respective value for Donor Lifetime with FRET & Donor Lifetime and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Efficiency of Energy Transfer?

In this formula, Efficiency of Energy Transfer uses Donor Lifetime with FRET & Donor Lifetime. We can use 5 other way(s) to calculate the same, which is/are as follows -

Efficiency of Energy Transfer = 1/(1+(Donor to Acceptor Distance/Forster Critical Distance)^6)
Efficiency of Energy Transfer = Rate of Energy Transfer/(Rate of Energy Transfer+Rate of Non radiative Transitions+Rate of Radiative Transitions)
Efficiency of Energy Transfer = Rate of Energy Transfer/(1/Donor Lifetime with FRET)
Efficiency of Energy Transfer = 1-(Fluorescence Intensity with FRET/Fluorescence intensity)
Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET)

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