Efficiency of Energy Transfer using Fluorescence Intensity of Donor Calculator

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Physical spectroscopy

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Fluoroscence and Phosphorescence

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Förster resonance energy transfer

✖Fluorescence Intensity with FRET is the donor fluorescence intensities with an acceptor.ⓘ Fluorescence Intensity with FRET [F_DA]			+10% -10%
✖The Fluorescence intensity is the fluorescence intensity of the donor in absence of FRET.ⓘ Fluorescence intensity [F_D]			+10% -10%

✖The Efficiency of Energy Transfer describes the efficiency of energy transfer between two light-sensitive molecules (chromophores).ⓘ Efficiency of Energy Transfer using Fluorescence Intensity of Donor [E]

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Formula

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Efficiency of Energy Transfer using Fluorescence Intensity of Donor

Formula

`"E" = 1-("F"_{"DA"}/"F"_{"D"})`

Example

`"0.7"=1-("0.003"/"0.01")`

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Efficiency of Energy Transfer using Fluorescence Intensity of Donor Solution

STEP 0: Pre-Calculation Summary

Formula Used

Efficiency of Energy Transfer = 1-(Fluorescence Intensity with FRET/Fluorescence intensity)
E = 1-(F_DA/F_D)
This formula uses 3 Variables

Variables Used

Efficiency of Energy Transfer - The Efficiency of Energy Transfer describes the efficiency of energy transfer between two light-sensitive molecules (chromophores).
Fluorescence Intensity with FRET - Fluorescence Intensity with FRET is the donor fluorescence intensities with an acceptor.
Fluorescence intensity - The Fluorescence intensity is the fluorescence intensity of the donor in absence of FRET.

STEP 1: Convert Input(s) to Base Unit

Fluorescence Intensity with FRET: 0.003 --> No Conversion Required
Fluorescence intensity: 0.01 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

E = 1-(F_DA/F_D) --> 1-(0.003/0.01)

Evaluating ... ...

E = 0.7

STEP 3: Convert Result to Output's Unit

0.7 --> No Conversion Required

FINAL ANSWER

0.7 <-- Efficiency of Energy Transfer

(Calculation completed in 00.004 seconds)

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national institute of technology meghalaya (NIT Meghalaya), Shillong

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< 11 Förster resonance energy transfer Calculators

Forster Critical Distance

Go Forster Critical Distance = 0.0211*((Refractive Index of Medium)^(-4)*(Fluorescence Quantum Yield without FRET)*(Orientation Factor)*(Spectral Overlap Integral))^(1/6)

Efficiency of Energy Transfer using Rate of Energy Transfer

Go Efficiency of Energy Transfer = Rate of Energy Transfer/(Rate of Energy Transfer+Rate of Non radiative Transitions+Rate of Radiative Transitions)

Donor Lifetime with FRET using Rate of Energy and Transitions

Go Donor Lifetime with FRET = 1/(Rate of Energy Transfer+Rate of Radiative Transitions+Rate of Non radiative Transitions)

Rate of Energy Transfer using Distances and Donor Lifetime

Go Rate of Energy Transfer = (1/Donor Lifetime)*(Forster Critical Distance/Donor to Acceptor Distance)^6

Efficiency of Energy Transfer using Photobleaching Decay Time Constant

Go Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET)

Efficiency of Energy Transfer using Distances

Go Efficiency of Energy Transfer = 1/(1+(Donor to Acceptor Distance/Forster Critical Distance)^6)

Efficiency of Energy Transfer using Fluorescence Intensity of Donor

Go Efficiency of Energy Transfer = 1-(Fluorescence Intensity with FRET/Fluorescence intensity)

Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime

Go Efficiency of Energy Transfer = Rate of Energy Transfer/(1/Donor Lifetime with FRET)

Donor Lifetime using Rates of Transitions

Go Donor Lifetime = 1/(Rate of Radiative Transitions+Rate of Non radiative Transitions)

Fluorescence Quantum Yield in FRET

Go Fluorescence Quantum Yield = Number of Photons Emitted/Number of Photons Absorbed

Efficiency of Energy Transfer using Donor Lifetime

Go Efficiency of Energy Transfer = 1-(Donor Lifetime with FRET/Donor Lifetime)

Efficiency of Energy Transfer using Fluorescence Intensity of Donor Formula

Efficiency of Energy Transfer = 1-(Fluorescence Intensity with FRET/Fluorescence intensity)
E = 1-(F_DA/F_D)

What are chromophores?

Chromophores are regions in a molecule where the energy difference between two molecular orbitals falls within the visible spectrum.

How to Calculate Efficiency of Energy Transfer using Fluorescence Intensity of Donor?

Efficiency of Energy Transfer using Fluorescence Intensity of Donor calculator uses Efficiency of Energy Transfer = 1-(Fluorescence Intensity with FRET/Fluorescence intensity) to calculate the Efficiency of Energy Transfer, The Efficiency of Energy Transfer using Fluorescence Intensity of Donor formula is defined as the ratio of the difference of fluorescence intensity of donor in presence and absence of acceptor to the fluorescence intensity in presence of FRET. Efficiency of Energy Transfer is denoted by E symbol.

How to calculate Efficiency of Energy Transfer using Fluorescence Intensity of Donor using this online calculator? To use this online calculator for Efficiency of Energy Transfer using Fluorescence Intensity of Donor, enter Fluorescence Intensity with FRET (F_DA) & Fluorescence intensity (F_D) and hit the calculate button. Here is how the Efficiency of Energy Transfer using Fluorescence Intensity of Donor calculation can be explained with given input values -> -2 = 1-(0.003/0.01).

FAQ

What is Efficiency of Energy Transfer using Fluorescence Intensity of Donor?

The Efficiency of Energy Transfer using Fluorescence Intensity of Donor formula is defined as the ratio of the difference of fluorescence intensity of donor in presence and absence of acceptor to the fluorescence intensity in presence of FRET and is represented as E = 1-(F_DA/F_D) or Efficiency of Energy Transfer = 1-(Fluorescence Intensity with FRET/Fluorescence intensity). Fluorescence Intensity with FRET is the donor fluorescence intensities with an acceptor & The Fluorescence intensity is the fluorescence intensity of the donor in absence of FRET.

How to calculate Efficiency of Energy Transfer using Fluorescence Intensity of Donor?

The Efficiency of Energy Transfer using Fluorescence Intensity of Donor formula is defined as the ratio of the difference of fluorescence intensity of donor in presence and absence of acceptor to the fluorescence intensity in presence of FRET is calculated using Efficiency of Energy Transfer = 1-(Fluorescence Intensity with FRET/Fluorescence intensity). To calculate Efficiency of Energy Transfer using Fluorescence Intensity of Donor, you need Fluorescence Intensity with FRET (F_DA) & Fluorescence intensity (F_D). With our tool, you need to enter the respective value for Fluorescence Intensity with FRET & Fluorescence intensity and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Efficiency of Energy Transfer?

In this formula, Efficiency of Energy Transfer uses Fluorescence Intensity with FRET & Fluorescence intensity. We can use 5 other way(s) to calculate the same, which is/are as follows -

Efficiency of Energy Transfer = 1/(1+(Donor to Acceptor Distance/Forster Critical Distance)^6)
Efficiency of Energy Transfer = Rate of Energy Transfer/(Rate of Energy Transfer+Rate of Non radiative Transitions+Rate of Radiative Transitions)
Efficiency of Energy Transfer = Rate of Energy Transfer/(1/Donor Lifetime with FRET)
Efficiency of Energy Transfer = 1-(Donor Lifetime with FRET/Donor Lifetime)
Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET)

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