Fluorescence Quantum Yield in FRET Calculator

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Förster resonance energy transfer

✖Number of Photons Emitted is the simple number of photons, which are emitted in the fluorescence process.ⓘ Number of Photons Emitted [nP_e]			+10% -10%
✖Number of Photons Absorbed is the quantity of photons that is absorbed in the process.ⓘ Number of Photons Absorbed [nP_a]			+10% -10%

✖The Fluorescence Quantum Yield is defined as the ratio of the number of photons emitted to the number of photons absorbed.ⓘ Fluorescence Quantum Yield in FRET [φ]

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Formula

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Fluorescence Quantum Yield in FRET

Formula

`"φ" = "nP"_{"e"}/"nP"_{"a"}`

Example

`"0.99996"="100000"/"100004"`

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Fluorescence Quantum Yield in FRET Solution

STEP 0: Pre-Calculation Summary

Formula Used

Fluorescence Quantum Yield = Number of Photons Emitted/Number of Photons Absorbed
φ = nP_e/nP_a
This formula uses 3 Variables

Variables Used

Fluorescence Quantum Yield - The Fluorescence Quantum Yield is defined as the ratio of the number of photons emitted to the number of photons absorbed.
Number of Photons Emitted - Number of Photons Emitted is the simple number of photons, which are emitted in the fluorescence process.
Number of Photons Absorbed - Number of Photons Absorbed is the quantity of photons that is absorbed in the process.

STEP 1: Convert Input(s) to Base Unit

Number of Photons Emitted: 100000 --> No Conversion Required
Number of Photons Absorbed: 100004 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

φ = nP_e/nP_a --> 100000/100004

Evaluating ... ...

φ = 0.999960001599936

STEP 3: Convert Result to Output's Unit

0.999960001599936 --> No Conversion Required

FINAL ANSWER

0.999960001599936 ≈ 0.99996 <-- Fluorescence Quantum Yield

(Calculation completed in 00.004 seconds)

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Home » Chemistry » Physical Chemistry » Physical spectroscopy » Emission Spectroscopy » Fluoroscence and Phosphorescence » Förster resonance energy transfer » Fluorescence Quantum Yield in FRET

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< 11 Förster resonance energy transfer Calculators

Forster Critical Distance

Go Forster Critical Distance = 0.0211*((Refractive Index of Medium)^(-4)*(Fluorescence Quantum Yield without FRET)*(Orientation Factor)*(Spectral Overlap Integral))^(1/6)

Efficiency of Energy Transfer using Rate of Energy Transfer

Go Efficiency of Energy Transfer = Rate of Energy Transfer/(Rate of Energy Transfer+Rate of Non radiative Transitions+Rate of Radiative Transitions)

Donor Lifetime with FRET using Rate of Energy and Transitions

Go Donor Lifetime with FRET = 1/(Rate of Energy Transfer+Rate of Radiative Transitions+Rate of Non radiative Transitions)

Rate of Energy Transfer using Distances and Donor Lifetime

Go Rate of Energy Transfer = (1/Donor Lifetime)*(Forster Critical Distance/Donor to Acceptor Distance)^6

Efficiency of Energy Transfer using Photobleaching Decay Time Constant

Go Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET)

Efficiency of Energy Transfer using Distances

Go Efficiency of Energy Transfer = 1/(1+(Donor to Acceptor Distance/Forster Critical Distance)^6)

Efficiency of Energy Transfer using Fluorescence Intensity of Donor

Go Efficiency of Energy Transfer = 1-(Fluorescence Intensity with FRET/Fluorescence intensity)

Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime

Go Efficiency of Energy Transfer = Rate of Energy Transfer/(1/Donor Lifetime with FRET)

Donor Lifetime using Rates of Transitions

Go Donor Lifetime = 1/(Rate of Radiative Transitions+Rate of Non radiative Transitions)

Fluorescence Quantum Yield in FRET

Go Fluorescence Quantum Yield = Number of Photons Emitted/Number of Photons Absorbed

Efficiency of Energy Transfer using Donor Lifetime

Go Efficiency of Energy Transfer = 1-(Donor Lifetime with FRET/Donor Lifetime)

Fluorescence Quantum Yield in FRET Formula

Fluorescence Quantum Yield = Number of Photons Emitted/Number of Photons Absorbed
φ = nP_e/nP_a

What is Fluorescence quantum yield?

Fluorescence quantum yield is a measure of the efficiency of photon emission through fluorescence. It is defined as the ratio of the number of molecules that fluoresce to the total number of excited molecules, or the ratio of photons emitted to photons absorbed.

How to Calculate Fluorescence Quantum Yield in FRET?

Fluorescence Quantum Yield in FRET calculator uses Fluorescence Quantum Yield = Number of Photons Emitted/Number of Photons Absorbed to calculate the Fluorescence Quantum Yield, The Fluorescence Quantum Yield in FRET formula is defined as the ratio of the number of photons emitted to the number of photons absorbed. Fluorescence Quantum Yield is denoted by φ symbol.

How to calculate Fluorescence Quantum Yield in FRET using this online calculator? To use this online calculator for Fluorescence Quantum Yield in FRET, enter Number of Photons Emitted (nP_e) & Number of Photons Absorbed (nP_a) and hit the calculate button. Here is how the Fluorescence Quantum Yield in FRET calculation can be explained with given input values -> 0.99996 = 100000/100004.

FAQ

What is Fluorescence Quantum Yield in FRET?

The Fluorescence Quantum Yield in FRET formula is defined as the ratio of the number of photons emitted to the number of photons absorbed and is represented as φ = nP_e/nP_a or Fluorescence Quantum Yield = Number of Photons Emitted/Number of Photons Absorbed. Number of Photons Emitted is the simple number of photons, which are emitted in the fluorescence process & Number of Photons Absorbed is the quantity of photons that is absorbed in the process.

How to calculate Fluorescence Quantum Yield in FRET?

The Fluorescence Quantum Yield in FRET formula is defined as the ratio of the number of photons emitted to the number of photons absorbed is calculated using Fluorescence Quantum Yield = Number of Photons Emitted/Number of Photons Absorbed. To calculate Fluorescence Quantum Yield in FRET, you need Number of Photons Emitted (nP_e) & Number of Photons Absorbed (nP_a). With our tool, you need to enter the respective value for Number of Photons Emitted & Number of Photons Absorbed and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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