Maximum Rate in presence of Noncompetitive Inhibitor Calculator

✖Apparent Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration in presence of non-competitive inhibitor.ⓘ Apparent Maximum Rate [Vmax^app]			+10% -10%
✖The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.ⓘ Inhibitor Concentration [I]			+10% -10%
✖The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.ⓘ Enzyme Inhibitor Dissociation Constant [K_i]			+10% -10%

✖The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.ⓘ Maximum Rate in presence of Noncompetitive Inhibitor [V_max]

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Formula

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Maximum Rate in presence of Noncompetitive Inhibitor

Formula

`"V"_{"max"} = ("Vmax"^{"app"}*(1+("I"/"K"_{"i"})))`

Example

`"30.94737mol/L*s"=("21mol/L*s"*(1+("9mol/L"/"19mol/L")))`

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Maximum Rate in presence of Noncompetitive Inhibitor Solution

STEP 0: Pre-Calculation Summary

Formula Used

Maximum Rate = (Apparent Maximum Rate*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))
V_max = (Vmax^app*(1+(I/K_i)))
This formula uses 4 Variables

Variables Used

Maximum Rate - (Measured in Mole per Cubic Meter Second) - The Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration.
Apparent Maximum Rate - (Measured in Mole per Cubic Meter Second) - Apparent Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration in presence of non-competitive inhibitor.
Inhibitor Concentration - (Measured in Mole per Cubic Meter) - The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system.
Enzyme Inhibitor Dissociation Constant - (Measured in Mole per Cubic Meter) - The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.

STEP 1: Convert Input(s) to Base Unit

Apparent Maximum Rate: 21 Mole per Liter Second --> 21000 Mole per Cubic Meter Second (Check conversion here)
Inhibitor Concentration: 9 Mole per Liter --> 9000 Mole per Cubic Meter (Check conversion here)
Enzyme Inhibitor Dissociation Constant: 19 Mole per Liter --> 19000 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

V_max = (Vmax^app*(1+(I/K_i))) --> (21000*(1+(9000/19000)))

Evaluating ... ...

V_max = 30947.3684210526

STEP 3: Convert Result to Output's Unit

30947.3684210526 Mole per Cubic Meter Second -->30.9473684210526 Mole per Liter Second (Check conversion here)

FINAL ANSWER

30.9473684210526 ≈ 30.94737 Mole per Liter Second <-- Maximum Rate

(Calculation completed in 00.004 seconds)

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< 9 Noncompetitive Inhibitor Calculators

Dissociation Constant given Enzyme Substrate Complex Concentration

Go Enzyme Inhibitor Dissociation Constant = Inhibitor Concentration/(((((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/Michaelis Constant)-1)

Apparent Initial Enzyme Concentration in Presence of Noncompetitive Inhibitor

Go Apparent Initial Enzyme Concentration = (Initial Enzyme Concentration/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))

Initial Enzyme Concentration in presence of Noncompetitive Inhibitor

Go Initial Enzyme Concentration = (Apparent Initial Enzyme Concentration*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))

Dissociation Constant given Apparent Initial Enzyme Concentration

Go Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1))

Apparent Michaelis Menten constant given Inhibitor's Dissociation Constant

Go Apparent Michaelis Constant = Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))

Apparent Maximum Rate in presence of Noncompetitive Inhibitor

Go Apparent Maximum Rate = (Maximum Rate/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))

Dissociation Constant in presence of Noncompetitive Inhibitor

Go Enzyme Inhibitor Dissociation Constant = (Inhibitor Concentration/((Maximum Rate/Apparent Maximum Rate)-1))

Maximum Rate in presence of Noncompetitive Inhibitor

Go Maximum Rate = (Apparent Maximum Rate*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))

Inhibitor Concentration in presence of Noncompetitive Inhibitor

Go Inhibitor Concentration = ((Maximum Rate/Apparent Maximum Rate)-1)*Enzyme Inhibitor Dissociation Constant

< 25 Important Formulas on Enzyme Kinetics Calculators

Final Rate Constant for Competitive Inhibition of Enzyme Catalysis

Go Final Rate Constant for Catalysis = (Initial Reaction Rate*(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration))/(Initial Enzyme Concentration*Substrate Concentration)

Inhibitor Concentration for Competitive Inhibition of Enzyme Catalysis

Go Inhibitor Concentration given IEC = (((((Final Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant

Michaelis Constant in Competitive Inhibition given Enzyme Substrate Complex Concentration

Go Michaelis Constant = (((Initial Enzyme Concentration*Substrate Concentration)/Enzyme Substrate Complex Concentration)-Substrate Concentration)/(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))

Enzyme Substrate Complex Concentration for Competitive Inhibition of Enzyme Catalysis

Go Enzyme Substrate Complex Concentration = (Substrate Concentration*Initial Enzyme Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration)

Inhibitor Concentration in Competitive Inhibition given Maximum Rate of System

Go Inhibitor Concentration given Max Rate = (((((Maximum Rate*Substrate Concentration)/Initial Reaction Rate)-Substrate Concentration)/Michaelis Constant)-1)*Enzyme Inhibitor Dissociation Constant

Initial Rate in Competitive Inhibition given Maximum Rate of system

Go Initial Reaction Rate in CI = (Maximum Rate*Substrate Concentration)/(Michaelis Constant*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))+Substrate Concentration)

Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants

Go Catalyst Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration)

Catalytic Rate Constant from Michaelis Menten Kinetics Equation

Go Catalytic Rate Constant for MM = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/(Initial Enzyme Concentration*Substrate Concentration)

Enzyme Concentration from Michaelis Menten Kinetics equation

Go Initial Concentration of Enzyme = (Initial Reaction Rate*(Michaelis Constant+Substrate Concentration))/(Catalytic Rate Constant*Substrate Concentration)

Substrate Concentration given Catalytic Rate Constant and Initial Enzyme Concentration

Go Concentration of Substrate = (Michaelis Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Initial Enzyme Concentration given Dissociation Rate Constant

Go Enzyme Concentration Initially = (Enzyme Substrate Complex Concentration*(Dissociation Rate Constant+Substrate Concentration))/(Substrate Concentration)

Initial Reaction Rate given Dissociation Rate Constant

Go Initial Reaction Rate given DRC = (Maximum Rate*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)

Maximum Rate given Dissociation Rate Constant

Go Maximum Rate given DRC = (Initial Reaction Rate*(Dissociation Rate Constant+Substrate Concentration))/Substrate Concentration

Inhibitor Concentration given Apparent Initial Enzyme Concentration

Go Inhibitor Concentration for CI = ((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1)*Enzyme Inhibitor Dissociation Constant

Initial Concentration of Enzyme in presence of Inhibitor by Enzyme Conservation Law

Go Enzyme Concentration Initially = (Catalyst Concentration+Enzyme Substrate Complex Concentration+Enzyme Inhibitor Complex Concentration)

Michaelis Constant given Forward, Reverse, and Catalytic Rate Constants

Go Michaelis Constant = (Reverse Rate Constant+Catalytic Rate Constant)/Forward Rate Constant

Inhibitor Concentration given Enzyme Substrate Modifying Factor

Go Inhibitor Concentration = (Enzyme Substrate Modifying Factor-1)*Enzyme Substrate Dissociation Constant

Modifying Factor of Enzyme Substrate Complex

Go Enzyme Substrate Modifying Factor = 1+(Inhibitor Concentration/Enzyme Substrate Dissociation Constant)

Dissociation Constant of Enzyme given Modifying Factor of Enzyme

Go Enzyme Inhibitor Dissociation Constant given MF = Inhibitor Concentration/(Enzyme Modifying Factor-1)

Initial Rate of System given Rate Constant and Enzyme Substrate Complex Concentration

Go Initial Reaction Rate given RC = Final Rate Constant*Enzyme Substrate Complex Concentration

Forward Rate Constant given Dissociation Rate Constant

Go Forward Rate Constant = (Reverse Rate Constant/Dissociation Rate Constant)

Dissociation Rate Constant in Enzymatic Reaction Mechanism

Go Dissociation Rate Constant = Reverse Rate Constant/Forward Rate Constant

Initial Enzyme Concentration if Substrate Concentration is Higher than Michaelis Constant

Go Enzyme Concentration Initially = Maximum Rate/Catalytic Rate Constant

Catalytic Rate Constant if Substrate Concentration is higher than Michaelis Constant

Go Catalytic Rate Constant = Maximum Rate/Initial Enzyme Concentration

Maximum Rate if Substrate Concentration is Higher than Michaelis Constant

Go Maximum Rate = Catalytic Rate Constant*Initial Enzyme Concentration

Maximum Rate in presence of Noncompetitive Inhibitor Formula

Maximum Rate = (Apparent Maximum Rate*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant)))
V_max = (Vmax^app*(1+(I/K_i)))

What is Non-competitive inhibition?

Non-competitive inhibition is a type of enzyme inhibition where the inhibitor reduces the activity of the enzyme and binds equally well to the enzyme whether or not it has already bound the substrate. In the presence of a non-competitive inhibitor, the apparent enzyme affinity is equivalent to the actual affinity because the inhibitor binds to both the enzyme and the enzyme-substrate complex equally so that the equilibrium is maintained. However, since some enzyme is always inhibited from converting the substrate to product, the effective enzyme concentration is lowered.

How to Calculate Maximum Rate in presence of Noncompetitive Inhibitor?

Maximum Rate in presence of Noncompetitive Inhibitor calculator uses Maximum Rate = (Apparent Maximum Rate*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))) to calculate the Maximum Rate, The Maximum rate in presence of noncompetitive inhibitor formula is defined as relation with the apparent maximum rate of the system, inhibitor concentration and with dissociation constant. Maximum Rate is denoted by V_max symbol.

How to calculate Maximum Rate in presence of Noncompetitive Inhibitor using this online calculator? To use this online calculator for Maximum Rate in presence of Noncompetitive Inhibitor, enter Apparent Maximum Rate (Vmax^app), Inhibitor Concentration (I) & Enzyme Inhibitor Dissociation Constant (K_i) and hit the calculate button. Here is how the Maximum Rate in presence of Noncompetitive Inhibitor calculation can be explained with given input values -> 0.030947 = (21000*(1+(9000/19000))).

FAQ

What is Maximum Rate in presence of Noncompetitive Inhibitor?

The Maximum rate in presence of noncompetitive inhibitor formula is defined as relation with the apparent maximum rate of the system, inhibitor concentration and with dissociation constant and is represented as V_max = (Vmax^app*(1+(I/K_i))) or Maximum Rate = (Apparent Maximum Rate*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))). Apparent Maximum Rate is defined as the maximum speed achieved by the system at saturated substrate concentration in presence of non-competitive inhibitor, The Inhibitor concentration is defined as the number of moles of inhibitor present per liter of solution of the system & The Enzyme Inhibitor Dissociation Constant is measured by the method in which the inhibitor is titrated into a solution of enzyme and the heat released or absorbed is measured.

How to calculate Maximum Rate in presence of Noncompetitive Inhibitor?

The Maximum rate in presence of noncompetitive inhibitor formula is defined as relation with the apparent maximum rate of the system, inhibitor concentration and with dissociation constant is calculated using Maximum Rate = (Apparent Maximum Rate*(1+(Inhibitor Concentration/Enzyme Inhibitor Dissociation Constant))). To calculate Maximum Rate in presence of Noncompetitive Inhibitor, you need Apparent Maximum Rate (Vmax^app), Inhibitor Concentration (I) & Enzyme Inhibitor Dissociation Constant (K_i). With our tool, you need to enter the respective value for Apparent Maximum Rate, Inhibitor Concentration & Enzyme Inhibitor Dissociation Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Maximum Rate?

In this formula, Maximum Rate uses Apparent Maximum Rate, Inhibitor Concentration & Enzyme Inhibitor Dissociation Constant. We can use 1 other way(s) to calculate the same, which is/are as follows -

Maximum Rate = Catalytic Rate Constant*Initial Enzyme Concentration

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