Rate of Energy Transfer using Distances and Donor Lifetime Calculator

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Physical spectroscopy

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Emission Spectroscopy

Absorption Spectroscopy

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Vibrational Spectroscopy

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Fluoroscence and Phosphorescence

Quantum Yield and Singlet Llifetime

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Förster resonance energy transfer

✖The Donor Lifetime is the donor's fluorescence lifetime in the absence of the acceptor.ⓘ Donor Lifetime [ζ_D]			+10% -10%
✖The Forster Critical Distance is the distance at which the energy transfer efficiency is 50%.ⓘ Forster Critical Distance [R₀]			+10% -10%
✖Donor to Acceptor Distance is the distance between the donor and the acceptor molecules.ⓘ Donor to Acceptor Distance [r]			+10% -10%

✖Rate of Energy Transfer is simply the rate of energy transfer from a donor to an acceptor.ⓘ Rate of Energy Transfer using Distances and Donor Lifetime [K_T]

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Formula

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Rate of Energy Transfer using Distances and Donor Lifetime

Formula

`"K"_{"T"} = (1/"ζ"_{"D"})*("R"_{"0"}/"r")^6`

Example

`"26.2144"=(1/"0.01")*("40A"/"50A")^6`

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Rate of Energy Transfer using Distances and Donor Lifetime Solution

STEP 0: Pre-Calculation Summary

Formula Used

Rate of Energy Transfer = (1/Donor Lifetime)*(Forster Critical Distance/Donor to Acceptor Distance)^6
K_T = (1/ζ_D)*(R₀/r)^6
This formula uses 4 Variables

Variables Used

Rate of Energy Transfer - Rate of Energy Transfer is simply the rate of energy transfer from a donor to an acceptor.
Donor Lifetime - The Donor Lifetime is the donor's fluorescence lifetime in the absence of the acceptor.
Forster Critical Distance - (Measured in Meter) - The Forster Critical Distance is the distance at which the energy transfer efficiency is 50%.
Donor to Acceptor Distance - (Measured in Meter) - Donor to Acceptor Distance is the distance between the donor and the acceptor molecules.

STEP 1: Convert Input(s) to Base Unit

Donor Lifetime: 0.01 --> No Conversion Required
Forster Critical Distance: 40 Angstrom --> 4E-09 Meter (Check conversion here)
Donor to Acceptor Distance: 50 Angstrom --> 5E-09 Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

K_T = (1/ζ_D)*(R₀/r)^6 --> (1/0.01)*(4E-09/5E-09)^6

Evaluating ... ...

K_T = 26.2144

STEP 3: Convert Result to Output's Unit

26.2144 --> No Conversion Required

FINAL ANSWER

26.2144 <-- Rate of Energy Transfer

(Calculation completed in 00.004 seconds)

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Home » Chemistry » Physical Chemistry » Physical spectroscopy » Emission Spectroscopy » Fluoroscence and Phosphorescence » Förster resonance energy transfer » Rate of Energy Transfer using Distances and Donor Lifetime

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< 11 Förster resonance energy transfer Calculators

Forster Critical Distance

Go Forster Critical Distance = 0.0211*((Refractive Index of Medium)^(-4)*(Fluorescence Quantum Yield without FRET)*(Orientation Factor)*(Spectral Overlap Integral))^(1/6)

Efficiency of Energy Transfer using Rate of Energy Transfer

Go Efficiency of Energy Transfer = Rate of Energy Transfer/(Rate of Energy Transfer+Rate of Non radiative Transitions+Rate of Radiative Transitions)

Donor Lifetime with FRET using Rate of Energy and Transitions

Go Donor Lifetime with FRET = 1/(Rate of Energy Transfer+Rate of Radiative Transitions+Rate of Non radiative Transitions)

Rate of Energy Transfer using Distances and Donor Lifetime

Go Rate of Energy Transfer = (1/Donor Lifetime)*(Forster Critical Distance/Donor to Acceptor Distance)^6

Efficiency of Energy Transfer using Photobleaching Decay Time Constant

Go Efficiency of Energy Transfer = 1-(Photobleaching Decay Time Constant/Photobleaching Decay Time Constant with FRET)

Efficiency of Energy Transfer using Distances

Go Efficiency of Energy Transfer = 1/(1+(Donor to Acceptor Distance/Forster Critical Distance)^6)

Efficiency of Energy Transfer using Fluorescence Intensity of Donor

Go Efficiency of Energy Transfer = 1-(Fluorescence Intensity with FRET/Fluorescence intensity)

Efficiency of Energy Transfer using Rate of Energy Transfer and Donor Lifetime

Go Efficiency of Energy Transfer = Rate of Energy Transfer/(1/Donor Lifetime with FRET)

Donor Lifetime using Rates of Transitions

Go Donor Lifetime = 1/(Rate of Radiative Transitions+Rate of Non radiative Transitions)

Fluorescence Quantum Yield in FRET

Go Fluorescence Quantum Yield = Number of Photons Emitted/Number of Photons Absorbed

Efficiency of Energy Transfer using Donor Lifetime

Go Efficiency of Energy Transfer = 1-(Donor Lifetime with FRET/Donor Lifetime)

Rate of Energy Transfer using Distances and Donor Lifetime Formula

Rate of Energy Transfer = (1/Donor Lifetime)*(Forster Critical Distance/Donor to Acceptor Distance)^6
K_T = (1/ζ_D)*(R₀/r)^6

What are Fluorophores?

A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several π bonds.

How to Calculate Rate of Energy Transfer using Distances and Donor Lifetime?

Rate of Energy Transfer using Distances and Donor Lifetime calculator uses Rate of Energy Transfer = (1/Donor Lifetime)*(Forster Critical Distance/Donor to Acceptor Distance)^6 to calculate the Rate of Energy Transfer, The Rate of Energy Transfer using Distances and Donor Lifetime formula is defined as multiplication of inverse of donor lifetime without FRET and to the 6th power of the ratio of forster critical distance to donor acceptor distance. Rate of Energy Transfer is denoted by K_T symbol.

How to calculate Rate of Energy Transfer using Distances and Donor Lifetime using this online calculator? To use this online calculator for Rate of Energy Transfer using Distances and Donor Lifetime, enter Donor Lifetime (ζ_D), Forster Critical Distance (R₀) & Donor to Acceptor Distance (r) and hit the calculate button. Here is how the Rate of Energy Transfer using Distances and Donor Lifetime calculation can be explained with given input values -> 2621.44 = (1/0.01)*(4E-09/5E-09)^6.

FAQ

What is Rate of Energy Transfer using Distances and Donor Lifetime?

The Rate of Energy Transfer using Distances and Donor Lifetime formula is defined as multiplication of inverse of donor lifetime without FRET and to the 6th power of the ratio of forster critical distance to donor acceptor distance and is represented as K_T = (1/ζ_D)*(R₀/r)^6 or Rate of Energy Transfer = (1/Donor Lifetime)*(Forster Critical Distance/Donor to Acceptor Distance)^6. The Donor Lifetime is the donor's fluorescence lifetime in the absence of the acceptor, The Forster Critical Distance is the distance at which the energy transfer efficiency is 50% & Donor to Acceptor Distance is the distance between the donor and the acceptor molecules.

How to calculate Rate of Energy Transfer using Distances and Donor Lifetime?

The Rate of Energy Transfer using Distances and Donor Lifetime formula is defined as multiplication of inverse of donor lifetime without FRET and to the 6th power of the ratio of forster critical distance to donor acceptor distance is calculated using Rate of Energy Transfer = (1/Donor Lifetime)*(Forster Critical Distance/Donor to Acceptor Distance)^6. To calculate Rate of Energy Transfer using Distances and Donor Lifetime, you need Donor Lifetime (ζ_D), Forster Critical Distance (R₀) & Donor to Acceptor Distance (r). With our tool, you need to enter the respective value for Donor Lifetime, Forster Critical Distance & Donor to Acceptor Distance and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

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