Retention Volume using Column Efficiency Calculator

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✖Width of Chromatographic Peak is the distance between points where lines tangent to the peak's left and right inflection points intersect the baseline.ⓘ Width of Chromatographic Peak [W_b]			+10% -10%
✖Column Efficiency, also known as plate count, is a measure of the dispersion of a peak.ⓘ Column Efficiency [N]			+10% -10%

✖The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column.ⓘ Retention Volume using Column Efficiency [V_R]

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Formula

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Retention Volume using Column Efficiency

Formula

`"V"_{"R"} = "W"_{"b"}*(sqrt("N"/16))`

Example

`"14.65755L"="25mm"*(sqrt("5.5"/16))`

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Retention Volume using Column Efficiency Solution

STEP 0: Pre-Calculation Summary

Formula Used

Retention Volume = Width of Chromatographic Peak*(sqrt(Column Efficiency/16))
V_R = W_b*(sqrt(N/16))
This formula uses 1 Functions, 3 Variables

Functions Used

sqrt - A square root function is a function that takes a non-negative number as an input and returns the square root of the given input number., sqrt(Number)

Variables Used

Retention Volume - (Measured in Cubic Meter) - The Retention Volume is defined as the volume of the mobile phase required to elute the solute from the column.
Width of Chromatographic Peak - (Measured in Meter) - Width of Chromatographic Peak is the distance between points where lines tangent to the peak's left and right inflection points intersect the baseline.
Column Efficiency - Column Efficiency, also known as plate count, is a measure of the dispersion of a peak.

STEP 1: Convert Input(s) to Base Unit

Width of Chromatographic Peak: 25 Millimeter --> 0.025 Meter (Check conversion here)
Column Efficiency: 5.5 --> No Conversion Required

STEP 2: Evaluate Formula

Substituting Input Values in Formula

V_R = W_b*(sqrt(N/16)) --> 0.025*(sqrt(5.5/16))

Evaluating ... ...

V_R = 0.0146575492494482

STEP 3: Convert Result to Output's Unit

0.0146575492494482 Cubic Meter -->14.6575492494482 Liter (Check conversion here)

FINAL ANSWER

14.6575492494482 ≈ 14.65755 Liter <-- Retention Volume

(Calculation completed in 00.006 seconds)

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National University of Judicial Science (NUJS), Kolkata

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Retention Volume using Column Efficiency Formula

Retention Volume = Width of Chromatographic Peak*(sqrt(Column Efficiency/16))
V_R = W_b*(sqrt(N/16))

What is Column Chromatography?

Column chromatography separates substances based on differential adsorption of compounds to the adsorbent as the compounds move through the column at different rates which allow them to get separated in fractions. This technique can be used on a small scale as well as large scale to purify materials that can be used in future experiments. This method is a type of adsorption chromatography technique.

How to Calculate Retention Volume using Column Efficiency?

Retention Volume using Column Efficiency calculator uses Retention Volume = Width of Chromatographic Peak*(sqrt(Column Efficiency/16)) to calculate the Retention Volume, The Retention Volume using Column Efficiency formula is defined as the volume of eluent carrier gas admitted to the column between the injection of the sample and the emergence of the peak maximum of the specified component. Retention Volume is denoted by V_R symbol.

How to calculate Retention Volume using Column Efficiency using this online calculator? To use this online calculator for Retention Volume using Column Efficiency, enter Width of Chromatographic Peak (W_b) & Column Efficiency (N) and hit the calculate button. Here is how the Retention Volume using Column Efficiency calculation can be explained with given input values -> 14657.55 = 0.025*(sqrt(5.5/16)).

FAQ

What is Retention Volume using Column Efficiency?

The Retention Volume using Column Efficiency formula is defined as the volume of eluent carrier gas admitted to the column between the injection of the sample and the emergence of the peak maximum of the specified component and is represented as V_R = W_b*(sqrt(N/16)) or Retention Volume = Width of Chromatographic Peak*(sqrt(Column Efficiency/16)). Width of Chromatographic Peak is the distance between points where lines tangent to the peak's left and right inflection points intersect the baseline & Column Efficiency, also known as plate count, is a measure of the dispersion of a peak.

How to calculate Retention Volume using Column Efficiency?

The Retention Volume using Column Efficiency formula is defined as the volume of eluent carrier gas admitted to the column between the injection of the sample and the emergence of the peak maximum of the specified component is calculated using Retention Volume = Width of Chromatographic Peak*(sqrt(Column Efficiency/16)). To calculate Retention Volume using Column Efficiency, you need Width of Chromatographic Peak (W_b) & Column Efficiency (N). With our tool, you need to enter the respective value for Width of Chromatographic Peak & Column Efficiency and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Retention Volume?

In this formula, Retention Volume uses Width of Chromatographic Peak & Column Efficiency. We can use 1 other way(s) to calculate the same, which is/are as follows -

Retention Volume = Void Volume*(Retention Factor+1)

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