Enzyme Substrate Complex Concentration given Dissociation Rate Constant Calculator

✖The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.ⓘ Initial Enzyme Concentration [[E₀]]			+10% -10%
✖The Substrate Concentration is the number of moles of substrate per liter solution.ⓘ Substrate Concentration [S]			+10% -10%
✖The Dissociation Rate Constant is the ratio of reverse and forward rate constant.ⓘ Dissociation Rate Constant [K_D]			+10% -10%

✖The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.ⓘ Enzyme Substrate Complex Concentration given Dissociation Rate Constant [ES]

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Formula

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Enzyme Substrate Complex Concentration given Dissociation Rate Constant

Formula

`"ES" = (("[E"_{"0"}"]")*"S")/("K"_{"D"}+"S")`

Example

`"20.83333mol/L"=("100mol/L"*"1.5mol/L")/("5.7mol/L"+"1.5mol/L")`

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Enzyme Substrate Complex Concentration given Dissociation Rate Constant Solution

STEP 0: Pre-Calculation Summary

Formula Used

Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)
ES = ([E₀]*S)/(K_D+S)
This formula uses 4 Variables

Variables Used

Enzyme Substrate Complex Concentration - (Measured in Mole per Cubic Meter) - The Enzyme Substrate Complex Concentration is defined as the concentration of intermediate formed from the reaction of enzyme and substrate.
Initial Enzyme Concentration - (Measured in Mole per Cubic Meter) - The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction.
Substrate Concentration - (Measured in Mole per Cubic Meter) - The Substrate Concentration is the number of moles of substrate per liter solution.
Dissociation Rate Constant - (Measured in Mole per Cubic Meter) - The Dissociation Rate Constant is the ratio of reverse and forward rate constant.

STEP 1: Convert Input(s) to Base Unit

Initial Enzyme Concentration: 100 Mole per Liter --> 100000 Mole per Cubic Meter (Check conversion here)
Substrate Concentration: 1.5 Mole per Liter --> 1500 Mole per Cubic Meter (Check conversion here)
Dissociation Rate Constant: 5.7 Mole per Liter --> 5700 Mole per Cubic Meter (Check conversion here)

STEP 2: Evaluate Formula

Substituting Input Values in Formula

ES = ([E₀]*S)/(K_D+S) --> (100000*1500)/(5700+1500)

Evaluating ... ...

ES = 20833.3333333333

STEP 3: Convert Result to Output's Unit

20833.3333333333 Mole per Cubic Meter -->20.8333333333333 Mole per Liter (Check conversion here)

FINAL ANSWER

20.8333333333333 ≈ 20.83333 Mole per Liter <-- Enzyme Substrate Complex Concentration

(Calculation completed in 00.004 seconds)

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< 21 Complex Concentration Calculators

Enzyme Substrate Complex Concentration in Instantaneous Chemical Equilibrium

Go Enzyme Substrate Complex Concentration = (Forward Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/(Reverse Rate Constant+(Forward Rate Constant*Substrate Concentration))

Initial Enzyme Concentration in Enzymatic Reaction Mechanism

Go Initial Enzyme Concentration = ((Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration))+Enzyme Substrate Complex Concentration

Substrate Concentration in Enzymatic Reaction Mechanism

Go Substrate Concentration = (Reverse Rate Constant*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration))

Enzyme Catalyst Concentration given Forward, Reverse, and Catalytic Rate Constants

Go Catalyst Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Substrate Concentration)

Substrate Concentration given Forward, Reverse, and Catalytic Rate Constants

Go Substrate Concentration = ((Reverse Rate Constant+Catalytic Rate Constant)*Enzyme Substrate Complex Concentration)/(Forward Rate Constant*Catalyst Concentration)

Initial Enzyme Concentration given Catalytic Rate Constant and Dissociation Rate Constants

Go Initial Enzyme Concentration = (Initial Reaction Rate*(Dissociation Rate Constant+Substrate Concentration))/(Catalytic Rate Constant*Substrate Concentration)

Substrate Concentration given Catalytic Rate Constant and Dissociation Rate Constants

Go Substrate Concentration = (Dissociation Rate Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Substrate Concentration given Catalytic Rate Constant and Initial Enzyme Concentration

Go Concentration of Substrate = (Michaelis Constant*Initial Reaction Rate)/((Catalytic Rate Constant*Initial Enzyme Concentration)-Initial Reaction Rate)

Substrate Concentration given Dissociation Rate Constant

Go Substrate Concentration = (Dissociation Rate Constant*Enzyme Substrate Complex Concentration)/(Initial Enzyme Concentration-Enzyme Substrate Complex Concentration)

Initial Enzyme Concentration given Dissociation Rate Constant

Go Enzyme Concentration Initially = (Enzyme Substrate Complex Concentration*(Dissociation Rate Constant+Substrate Concentration))/(Substrate Concentration)

Enzyme Substrate Complex Concentration given Dissociation Rate Constant

Go Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)

Substrate Concentration if Michaelis Constant is very Large than Substrate Concentration

Go Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Initial Enzyme Concentration)

Initial Enzyme Concentration at Low Substrate Concentration

Go Initial Enzyme Concentration = (Initial Reaction Rate*Michaelis Constant)/(Catalytic Rate Constant*Substrate Concentration)

Inhibitor Concentration given Apparent Initial Enzyme Concentration

Go Inhibitor Concentration for CI = ((Initial Enzyme Concentration/Apparent Initial Enzyme Concentration)-1)*Enzyme Inhibitor Dissociation Constant

Substrate Concentration given Maximum Rate and Dissociation Rate Constant

Go Substrate Concentration = (Dissociation Rate Constant*Initial Reaction Rate)/(Maximum Rate-Initial Reaction Rate)

Substrate Concentration given Maximum Rate at Low Concentration

Go Substrate Concentration = (Initial Reaction Rate*Michaelis Constant)/Maximum Rate

Inhibitor Concentration given Modifying Factor of Enzyme Substrate Complex

Go Inhibitor Concentration = (Enzyme Substrate Modifying Factor-1)*Enzyme Substrate Dissociation Constant

Inhibitor Concentration given Enzyme Substrate Modifying Factor

Go Inhibitor Concentration = (Enzyme Substrate Modifying Factor-1)*Enzyme Substrate Dissociation Constant

Inhibitor Concentration given Modifying Factor of Enzyme

Go Inhibitor Concentration = (Enzyme Modifying Factor-1)*Enzyme Inhibitor Dissociation Constant

Enzyme Substrate Complex Concentration given Rate Constant and Initial Rate

Go Enzyme Inhibitor Complex Concentration = (Initial Reaction Rate/Final Rate Constant)

Initial Enzyme Concentration given Rate Constant and Maximum Rate

Go Initial Enzyme Concentration = Maximum Rate/Final Rate Constant

Enzyme Substrate Complex Concentration given Dissociation Rate Constant Formula

Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration)
ES = ([E₀]*S)/(K_D+S)

What is Michaelis–Menten kinetics model?

In biochemistry, Michaelis–Menten kinetics is one of the best-known models of enzyme kinetics. Biochemical reactions involving a single substrate are often assumed to follow Michaelis–Menten kinetics, without regard to the model's underlying assumptions. The model takes the form of an equation describing the rate of enzymatic reactions, by relating the reaction rate of formation of product to the concentration of a substrate.

How to Calculate Enzyme Substrate Complex Concentration given Dissociation Rate Constant?

Enzyme Substrate Complex Concentration given Dissociation Rate Constant calculator uses Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration) to calculate the Enzyme Substrate Complex Concentration, The Enzyme substrate complex concentration given dissociation rate constant formula is defined as the relation with the dissociation rate constant and the concentration of enzyme and substrate. Enzyme Substrate Complex Concentration is denoted by ES symbol.

How to calculate Enzyme Substrate Complex Concentration given Dissociation Rate Constant using this online calculator? To use this online calculator for Enzyme Substrate Complex Concentration given Dissociation Rate Constant, enter Initial Enzyme Concentration ([E₀]), Substrate Concentration (S) & Dissociation Rate Constant (K_D) and hit the calculate button. Here is how the Enzyme Substrate Complex Concentration given Dissociation Rate Constant calculation can be explained with given input values -> 0.020833 = (100000*1500)/(5700+1500).

FAQ

What is Enzyme Substrate Complex Concentration given Dissociation Rate Constant?

The Enzyme substrate complex concentration given dissociation rate constant formula is defined as the relation with the dissociation rate constant and the concentration of enzyme and substrate and is represented as ES = ([E₀]*S)/(K_D+S) or Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration). The Initial Enzyme Concentration is defined as the concentration of enzyme at the start of the reaction, The Substrate Concentration is the number of moles of substrate per liter solution & The Dissociation Rate Constant is the ratio of reverse and forward rate constant.

How to calculate Enzyme Substrate Complex Concentration given Dissociation Rate Constant?

The Enzyme substrate complex concentration given dissociation rate constant formula is defined as the relation with the dissociation rate constant and the concentration of enzyme and substrate is calculated using Enzyme Substrate Complex Concentration = (Initial Enzyme Concentration*Substrate Concentration)/(Dissociation Rate Constant+Substrate Concentration). To calculate Enzyme Substrate Complex Concentration given Dissociation Rate Constant, you need Initial Enzyme Concentration ([E₀]), Substrate Concentration (S) & Dissociation Rate Constant (K_D). With our tool, you need to enter the respective value for Initial Enzyme Concentration, Substrate Concentration & Dissociation Rate Constant and hit the calculate button. You can also select the units (if any) for Input(s) and the Output as well.

How many ways are there to calculate Enzyme Substrate Complex Concentration?

In this formula, Enzyme Substrate Complex Concentration uses Initial Enzyme Concentration, Substrate Concentration & Dissociation Rate Constant. We can use 1 other way(s) to calculate the same, which is/are as follows -

Enzyme Substrate Complex Concentration = (Forward Rate Constant*Initial Enzyme Concentration*Substrate Concentration)/(Reverse Rate Constant+(Forward Rate Constant*Substrate Concentration))

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